CAJ | 학술논문

目的探讨2009年杭州市甲型H_1N_1流感的流行规律、临床表现和病原学特征.方法对2009年杭州市13家流感监测哨点医院的每日门诊流感样病例和甲型H_1N_1流感确诊病例的流行病学进行分析.采用RT-PCR方法对咽拭子中血凝素(HA)基因进行扩增和测序,并用DNASTAR软件进行序列分析.全文数据采用SPSS 12.0软件进行统计分析.率的比较采用x~2检验.结果流感样病例监测显示,第28周之前,疫情处于平稳状态,之后全市流感样病例逐渐增多.第35周时达到高峰,全市流感样病例就诊比例为7.47%(5442/72859).之后疫情回落,但到第41周时又再次回升,至第46周出现第二次高峰,全市流感样病例就诊比例达到11.32%(11034/97436).第38周以前,杭州市人群中主要流行株是季节性H_3N_2型,其次有少量的乙型和季节性流感H_1N_1型,到第44周,甲型H_1N_1流感成为唯一的优势流行株,主要易感人群是11-25岁的青少年,以学生居多.序列分析显示,2009年杭州市甲型H_1N_1流感病毒株与北美株、中国其他地区的毒株以及疫苗株同源性达到99%,关键位点高度保守,但与季节性流感病毒株同源性只有70%.结论 2009年杭州市流感疫情发展迅速,人群中流行的优势毒株为甲型流感H_1N_1型,易感人群为青少年,目前未发现变异株及高致病株,但仍需进一步监测.
목적 탐토2009년항주시갑형H_1N_1류감적류행규률、림상표현화병원학특정.방법 대2009년항주시13가류감감측초점의원적매일문진류감양병례화갑형H_1N_1류감학진병례적류행병학진행분석.채용RT-PCR방법대인식자중혈응소(HA)기인진행확증화측서,병용DNASTAR연건진행서렬분석.전문수거채용SPSS 12.0연건진행통계분석.솔적비교채용x~2검험.결과 류감양병례감측현시,제28주지전,역정처우평은상태,지후전시류감양병례축점증다.제35주시체도고봉,전시류감양병례취진비례위7.47%(5442/72859).지후역정회락,단도제41주시우재차회승,지제46주출현제이차고봉,전시류감양병례취진비례체도11.32%(11034/97436).제38주이전,항주시인군중주요류행주시계절성H_3N_2형,기차유소량적을형화계절성류감H_1N_1형,도제44주,갑형H_1N_1류감성위유일적우세류행주,주요역감인군시11-25세적청소년,이학생거다.서렬분석현시,2009년항주시갑형H_1N_1류감병독주여북미주、중국기타지구적독주이급역묘주동원성체도99%,관건위점고도보수,단여계절성류감병독주동원성지유70%.결론 2009년항주시류감역정발전신속,인군중류행적우세독주위갑형류감H_1N_1형,역감인군위청소년,목전미발현변이주급고치병주,단잉수진일보감측.
Objective To investigate the epidemiological,clinical and etiological features of influenza/influenza A(H_1N_1)in Hangzhou city in 2009.Methods Epidemiological data of patients with influeliza-1ike illness or influenza A(H_1N_1)from 13 sentinel hospitals in Hangzhou(2009)were analyzed.Hemagglutinin(HA)genes of H_1N_1 subtype influenza virus were amplified by RT-PCR and the sequence analysis were performed by DNAsTAR.SPSS 12.0 software was used for data processing and chi-square test was performed for difference comparison of rates.Results Epidemic cunre for influenza-like illness was comparatively flat during the first 28 weeks of 2009,then the percentage of patients with influenza-like illness increased and climbed to the peak at 35th week(7.47%,5 442/72 859).This number declined in the following weeks but rose again at 41st week.At 46th week,a second peak appeared and the percentage of Datients with influenza-1ike illness was 11.32%(11 034/97 436).Virological surveillance showed that the circulation of influenzz viruses before 38th week were mainly H_3N_2,B and seasonal influenza virus H_1N_1,while from 44th week on,the pandemic H_1N_1 influenza was the only predominated strain.Main snsceptible population in 2009 Hangzhou pandemic H_1N_1 influenza was people aged 11-25,and most of them were students.H_1N_1 strains of Hangzhou in 2009 were identical to strains in other parts of China,North American strains and vaccine candidate strain with 99%homology.but only 70%homology with seasonal inflnellZa virus.Conclusions The 2009 influenza A(H_1N_1)in Hangzhou has now entered a phase of rapid and susmined community transmission,susceptible populations are young adults.The predominated strain is the influenza A H_1N_1 type with high affinity and low pathogenicity to humans and no important variation.