中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2012年
5期
257-260
,共4页
李婕%任万华%石军%吴炜%陈智
李婕%任萬華%石軍%吳煒%陳智
리첩%임만화%석군%오위%진지
肝肿瘤%Th17细胞%调节性T细胞%免疫调节
肝腫瘤%Th17細胞%調節性T細胞%免疫調節
간종류%Th17세포%조절성T세포%면역조절
Liver neoplasms%Th17 cells%Regulatory T cell%Immunomodulation
目的 探讨原发性肝癌患者外周血Th17和CD4+ CD25+调节性T细胞的表达水平及其相关性.方法 选取2008年6月-2009年5月浙江大学医学院附属第一医院30例原发性肝癌患者和25名健康人群,采血并分离其外周血单个核细胞.利用流式细胞仪分别测定Th17和CD4+ CD25+ Foxp3+调节性T细胞的表达,采用t检验分析两组的表达差异.同时,采用Spearman检验对原发性肝癌患者外周血中Th17和CD4+ CD25+调节性T细胞的表达进行相关性分析.结果 健康对照组外周血中Th17细胞为(2.10±0.87)％,CD4+ CD25+调节性T细胞为(7.10±2.32)％,原发性肝癌组外周血中Th17细胞为(3.38±1.68)％,CD4+ CD25+调节性T细胞为(11.78±5.62)％,两组差异具有统计学意义(t=3.640和4.162,P值均＜0.01).原发性肝癌患者组外周血Th17细胞与CD4+ CD25+调节性T细胞表达呈正相关(r=0.821,P＜0.01).结论 原发性肝癌患者外周血Th17和CD4+ CD25+ Foxp3+调节性T细胞表达水平较高,二者呈正相关.CD4+ CD25+ Foxp3+调节性T细胞可能通过促进Th17细胞分化导致肿瘤的发生与发展.
目的 探討原髮性肝癌患者外週血Th17和CD4+ CD25+調節性T細胞的錶達水平及其相關性.方法 選取2008年6月-2009年5月浙江大學醫學院附屬第一醫院30例原髮性肝癌患者和25名健康人群,採血併分離其外週血單箇覈細胞.利用流式細胞儀分彆測定Th17和CD4+ CD25+ Foxp3+調節性T細胞的錶達,採用t檢驗分析兩組的錶達差異.同時,採用Spearman檢驗對原髮性肝癌患者外週血中Th17和CD4+ CD25+調節性T細胞的錶達進行相關性分析.結果 健康對照組外週血中Th17細胞為(2.10±0.87)％,CD4+ CD25+調節性T細胞為(7.10±2.32)％,原髮性肝癌組外週血中Th17細胞為(3.38±1.68)％,CD4+ CD25+調節性T細胞為(11.78±5.62)％,兩組差異具有統計學意義(t=3.640和4.162,P值均＜0.01).原髮性肝癌患者組外週血Th17細胞與CD4+ CD25+調節性T細胞錶達呈正相關(r=0.821,P＜0.01).結論 原髮性肝癌患者外週血Th17和CD4+ CD25+ Foxp3+調節性T細胞錶達水平較高,二者呈正相關.CD4+ CD25+ Foxp3+調節性T細胞可能通過促進Th17細胞分化導緻腫瘤的髮生與髮展.
목적 탐토원발성간암환자외주혈Th17화CD4+ CD25+조절성T세포적표체수평급기상관성.방법 선취2008년6월-2009년5월절강대학의학원부속제일의원30례원발성간암환자화25명건강인군,채혈병분리기외주혈단개핵세포.이용류식세포의분별측정Th17화CD4+ CD25+ Foxp3+조절성T세포적표체,채용t검험분석량조적표체차이.동시,채용Spearman검험대원발성간암환자외주혈중Th17화CD4+ CD25+조절성T세포적표체진행상관성분석.결과 건강대조조외주혈중Th17세포위(2.10±0.87)％,CD4+ CD25+조절성T세포위(7.10±2.32)％,원발성간암조외주혈중Th17세포위(3.38±1.68)％,CD4+ CD25+조절성T세포위(11.78±5.62)％,량조차이구유통계학의의(t=3.640화4.162,P치균＜0.01).원발성간암환자조외주혈Th17세포여CD4+ CD25+조절성T세포표체정정상관(r=0.821,P＜0.01).결론 원발성간암환자외주혈Th17화CD4+ CD25+ Foxp3+조절성T세포표체수평교고,이자정정상관.CD4+ CD25+ Foxp3+조절성T세포가능통과촉진Th17세포분화도치종류적발생여발전.
Objective To investigate the peripheral blood Th17 and CD4 + CD25 + regulatory T cell levels and their correlations in patients with primary hepatocellular carcinoma (PHC).Methods Peripheral blood samples were collected from 30 PHC patients and 25 healthy controls in the First Affiliated Hospital of Zhejiang University from June 2008 to May 2009.Mononuclear cells were isolated and the Th17 and CD4 + CD25 + regulatory T cells were detected by flow cytometry and compared between patients and controls by t test.Spearman test was performed to analyze the correlation of Th17 with CD4 + CD25 +regulatory T cell concentrations.Results The levels of Th17 and CD4 + CD25 + regulatory T cells in peripheral blood in healthy controls were (2.10 ± 0.87) ％ and (7.10 ± 2.32) ％ ; while those in PHC patients were (3.38±1.68)％ and (11.78±5.62)％ (t=3.640 and 4.162,P＜0.01).The level of Th17 cells was positively associated with that of CD4 + CD25 + regulatory T cells in PHC patients (r =0.821,P ＜0.01).Conclusion The levels of Th17 and CD4 + CD25 + regulatory T cells in peripheral blood are high in PHC patients and positively correlated with each other,which indicates that CD4 + CD25 + regulatory T cells may contribute to the disease progression and pathogenesis of carcinoma through inducing Th17 cells differentiation.
