中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2013年
5期
273-277
,共5页
汤磊%郜玉峰%戴金津%李旭%邹桂舟
湯磊%郜玉峰%戴金津%李旭%鄒桂舟
탕뢰%고옥봉%대금진%리욱%추계주
白细胞介素18%肝炎,乙型,慢性%免疫组织化学%临床特征
白細胞介素18%肝炎,乙型,慢性%免疫組織化學%臨床特徵
백세포개소18%간염,을형,만성%면역조직화학%림상특정
Interleukin-18%Hepatitis B,Chronic%Immnnohistochemistry%Clinical features
目的 检测慢性乙型肝炎(CHB)患者肝脏组织中白细胞介素18(IL-18)的表达并分析其与临床特征的相关性.方法 选取2012年1月至12月在安徽医科大学第二附属医院住院并行肝脏穿刺活检的CHB患者172例.应用免疫组化法检测患者肝组织中IL-18的表达情况,检测患者肝功能、HBV血清学标志物、HBV DNA载量、肝脏炎症分级和纤维化分期等.采用Spearman秩相关检验分析肝组织中IL-18表达与上述指标的相关性.结果 IL-18主要在肝细胞的细胞质中表达,其在健康人群的肝细胞中多呈阳性或强阳性表达,而在CHB患者则以弱阳性表达为主.两者肝组织中IL-18的表达差异有统计学意义(x2=42.124,P＜0.05).在CHB患者中,IL-18的表达随着患者ALT水平升高、炎症及纤维化程度的加重而升高(r =0.182,0.173和0.068,P＜0.05),但与HBV DNA水平和HBeAg无相关性(r=-0.05和0.041,P＞0.05).结论 CHB患者中,IL-18的表达强度与肝脏损伤程度及纤维化形成有关.
目的 檢測慢性乙型肝炎(CHB)患者肝髒組織中白細胞介素18(IL-18)的錶達併分析其與臨床特徵的相關性.方法 選取2012年1月至12月在安徽醫科大學第二附屬醫院住院併行肝髒穿刺活檢的CHB患者172例.應用免疫組化法檢測患者肝組織中IL-18的錶達情況,檢測患者肝功能、HBV血清學標誌物、HBV DNA載量、肝髒炎癥分級和纖維化分期等.採用Spearman秩相關檢驗分析肝組織中IL-18錶達與上述指標的相關性.結果 IL-18主要在肝細胞的細胞質中錶達,其在健康人群的肝細胞中多呈暘性或彊暘性錶達,而在CHB患者則以弱暘性錶達為主.兩者肝組織中IL-18的錶達差異有統計學意義(x2=42.124,P＜0.05).在CHB患者中,IL-18的錶達隨著患者ALT水平升高、炎癥及纖維化程度的加重而升高(r =0.182,0.173和0.068,P＜0.05),但與HBV DNA水平和HBeAg無相關性(r=-0.05和0.041,P＞0.05).結論 CHB患者中,IL-18的錶達彊度與肝髒損傷程度及纖維化形成有關.
목적 검측만성을형간염(CHB)환자간장조직중백세포개소18(IL-18)적표체병분석기여림상특정적상관성.방법 선취2012년1월지12월재안휘의과대학제이부속의원주원병행간장천자활검적CHB환자172례.응용면역조화법검측환자간조직중IL-18적표체정황,검측환자간공능、HBV혈청학표지물、HBV DNA재량、간장염증분급화섬유화분기등.채용Spearman질상관검험분석간조직중IL-18표체여상술지표적상관성.결과 IL-18주요재간세포적세포질중표체,기재건강인군적간세포중다정양성혹강양성표체,이재CHB환자칙이약양성표체위주.량자간조직중IL-18적표체차이유통계학의의(x2=42.124,P＜0.05).재CHB환자중,IL-18적표체수착환자ALT수평승고、염증급섬유화정도적가중이승고(r =0.182,0.173화0.068,P＜0.05),단여HBV DNA수평화HBeAg무상관성(r=-0.05화0.041,P＞0.05).결론 CHB환자중,IL-18적표체강도여간장손상정도급섬유화형성유관.
Objective To investigate the expression of interleukin 18 (IL-18) in liver tissues of patients with chronic hepatitis B (CHB) and its clinical significance.Methods Totally 172 CHB patients admitted in the Second Affiliated Hospital of Anhui Medical University from January 2012 to December 2012 were enrolled.The tissue specimens were obtained by liver biopsy from all patients,and 22 normal liver specimens were also collected as controls.Liver function,serum markers of HBV and HBV DNA load were measured and liver inflammation grades and fibrosis stages were assessed.Immunohistochemical method was used to detect the expression of IL-18 in liver tissues.Spearman rank correlation was used to analyze the correlations between IL-18 expression and the above indicators.Results IL-18 was mainly expressed in cytoplasm of liver cells.It was positive or strong positive in liver cells of healthy people,but weakly positive in liver cells of CHB patients.The difference of IL-18 expression between CHB patients and healthy people was of statistical significance (x2 =42.124,P ＜ 0.05).In CHB patients,the expression of IL-18 was gradually enhanced with the increasing of ALT,inflammation grades and fibrosis stages (r =0.182,0.173 and 0.068,P ＜0.05),but it was not correlated with HBV DNA load and HBeAg (r =-0.05 and 0.041,P ＞0.05).Conclusion In CHB patients,the expression of IL-18 may be involved in liver injury and fibrosis formation.
