CAJ | 학술논문

目的比较纸片扩散法和E-test法检测幽门螺杆菌对克拉霉素的耐药性,并进行纸片扩散法检测的标准化研究.方法从接受胃镜检查患者的胃黏膜活检样本中分离出44株幽门螺杆菌,用纸片扩散法和E-test法检测菌株对克拉霉素的敏感性,并建立直线回归方程评价两种方法的一致性.结果采用E-test法,克拉霉素的最小抑菌浓度(MIC)为0.016 ～ 256 μg/mL,菌株对克拉霉素的耐药率为27.3％ (12/44).采用纸片扩散法,抑菌圈直径是0～ 35 mm,两种方法具有良好的相关性(r2 =0.91,P＜0.01).根据回归方程,抑菌圈直径≥18 mm表示菌株对克拉霉素敏感,≤15 mm为耐药.根据上述折点,两种检测方法检测的一致率为100％.结论纸片扩散法与E-test法具有良好的一致性,在临床上可以替代E-test法.
목적 비교지편확산법화E-test법검측유문라간균대극랍매소적내약성,병진행지편확산법검측적표준화연구.방법 종접수위경검사환자적위점막활검양본중분리출44주유문라간균,용지편확산법화E-test법검측균주대극랍매소적민감성,병건립직선회귀방정평개량충방법적일치성.결과 채용E-test법,극랍매소적최소억균농도(MIC)위0.016 ～ 256 μg/mL,균주대극랍매소적내약솔위27.3％ (12/44).채용지편확산법,억균권직경시0～ 35 mm,량충방법구유량호적상관성(r2 =0.91,P＜0.01).근거회귀방정,억균권직경≥18 mm표시균주대극랍매소민감,≤15 mm위내약.근거상술절점,량충검측방법검측적일치솔위100％.결론 지편확산법여E-test법구유량호적일치성,재림상상가이체대E-test법.
Objective To compare disk diffusion with E-test methods for clarithromycin susceptibility testing of Helicobacter pylori (H.pylori).Methods A total of 44 strains of H.pylori were isolated from gastric mucosa biopsy from patients undergoing gastroscopic examination.Disk diffusion and E-test methods were used for clarithromycin susceptibility testing of H.pylori.The agreement of disk diffusion and E-test was assessed by linear regression analysis.Results The minimum inhibitory concentration (MIC) tested by E-test method ranged from 0.016 to 256 μg/mL,and drug resistance was observed in 12(27.3％) isolates.In range of 0-35 mm of inhibition diameter,the results of disk diffusion method were correlated well with the MICs obtained by E-test method (r2 =0.91,P ＜0.01).Regression analysis showed that with inhibition diameters≥ 18 mm as considered sensitive to clarithromycin and ≤ 15 mm as resistant,the agreement was 100％ between two methods.Conclusion The disk diffusion method is equivalent to the E-test method for clarithromycin susceptibility testing of H.pylori,which can be an alternative method for clinical application.