CAJ | 학술논문

目的探讨烟曲霉、白假丝酵母菌的不同菌体形态对脐血来源细胞因子诱导自然杀伤(NK)细胞内干扰素(IFN)-γ和白细胞介素(IL)-4表达的影响.方法将NK细胞与真菌(烟曲霉孢子或菌丝、白假丝酵母菌孢子或菌丝)按10∶1的比例直接混合或隔离培养分为若干实验组,另设一个NK细胞空白对照组.培养6h后通过流式细胞仪检测各组NK细胞内IFN-γ和IL-4的表达.采用方差分析或SNK-q检验比较各组间IFN-γ和IL-4的表达.结果空白对照组NK细胞内IFN-γ的表达率为(15.11±2.60)％,NK细胞与烟曲霉菌丝、白假丝酵母菌孢子和菌丝直接接触后细胞内IFN-γ的表达率分别为(20.12±0.53)％,(20.69±0.34)％,(20.8±0.37)％,间接接触后细胞内IFN-γ的表达率分别为(21.40±0.53)％,(20.57±1.09)％,(20.20 ±0.51)％,细胞内IFN-γ的表达较空白对照组显著升高(P均＜0.01);而NK细胞与烟曲霉孢子直接、间接接触作用后细胞内IFN-γ的表达率分别为(14.33±0.98)％,(14.97±1.53)％,与对照组比较差异无统计学意义(P值均＞0.05).空白对照组NK细胞内IL-4的表达率为(1.23±0.05)％,NK细胞与烟曲霉孢子和菌丝、白假丝酵母菌孢子和菌丝直接接触后细胞内IL-4的表达率分别为(1.25±0.06)％,(1.21±0.03)％,(1.22±0.46)％,(1.26±0.1 1)％,间接接触后细胞内IL-4的表达率分别为(1.21±0.06)％,(1.25±0.04)％,(1.27±0.03)％,(1.26±0.1)％,真菌刺激对NK细胞胞内IL-4的表达无显著影响(P值均＞0.05).结论真菌刺激可抑制NK细胞胞内IFN-γ的释放,但对IL-4的释放无显著影响.
목적 탐토연곡매、백가사효모균적불동균체형태대제혈래원세포인자유도자연살상(NK)세포내간우소(IFN)-γ화백세포개소(IL)-4표체적영향.방법 장NK세포여진균(연곡매포자혹균사、백가사효모균포자혹균사)안10∶1적비례직접혼합혹격리배양분위약간실험조,령설일개NK세포공백대조조.배양6h후통과류식세포의검측각조NK세포내IFN-γ화IL-4적표체.채용방차분석혹SNK-q검험비교각조간IFN-γ화IL-4적표체.결과 공백대조조NK세포내IFN-γ적표체솔위(15.11±2.60)％,NK세포여연곡매균사、백가사효모균포자화균사직접접촉후세포내IFN-γ적표체솔분별위(20.12±0.53)％,(20.69±0.34)％,(20.8±0.37)％,간접접촉후세포내IFN-γ적표체솔분별위(21.40±0.53)％,(20.57±1.09)％,(20.20 ±0.51)％,세포내IFN-γ적표체교공백대조조현저승고(P균＜0.01);이NK세포여연곡매포자직접、간접접촉작용후세포내IFN-γ적표체솔분별위(14.33±0.98)％,(14.97±1.53)％,여대조조비교차이무통계학의의(P치균＞0.05).공백대조조NK세포내IL-4적표체솔위(1.23±0.05)％,NK세포여연곡매포자화균사、백가사효모균포자화균사직접접촉후세포내IL-4적표체솔분별위(1.25±0.06)％,(1.21±0.03)％,(1.22±0.46)％,(1.26±0.1 1)％,간접접촉후세포내IL-4적표체솔분별위(1.21±0.06)％,(1.25±0.04)％,(1.27±0.03)％,(1.26±0.1)％,진균자격대NK세포포내IL-4적표체무현저영향(P치균＞0.05).결론 진균자격가억제NK세포포내IFN-γ적석방,단대IL-4적석방무현저영향.
Objective To investigate the effect of fungus Aspergillus fumigatus and Candida albicans on the expression of endocellular interferon-γ (IFN-γ) and interleukin-4 (IL-4) in cytokineinduced natural killer (NK) cells.Methods NK cells were cultured with Aspergillusfumigatus or Candida albicans by non-contact or direct-contact methods with a ratio of NK cells to fungus of 10 ∶ 1.The expressions of IFN-γ and IL-4 in NK cells were evaluated by flow cytometry after co-cultured for 6 h.Analysis of variance or SNK-q test was used to compare the expressions of IFN-γ and IL-4 among different groups.Results The IFN-γexpression rates in NK cells with direct contacting to Aspergillus fumigatus hyphae,or to different morphotypes of Candida albicans were (20.12 ± 0.53) ％,(20.69 ± 0.34) ％ and (20.8 ±0.37)％ respectively,while IFN-γexpression in NK cells with indirect contacting to fumigatus hyphae,or to different morphotypes of Candida albicans were (21.40 ± 0.53) ％,(20.57 ± 1.09) ％ and (20.20 ±0.51) ％ respectively,and all were significantly higher than that in the blank group [(15.11 ± 2.60) ％,all P ＞ 0.05].The IFN-γ expression rates in the Aspergillus fumigatus spores direct and indirect contacting groups were (14.33 ± 0.98) ％ and (14.97 ± 1.53) ％,which were not of significant difference compared with the blank group (P ＞ 0.05).The IL-4 expression rates in NK cells with direct contacting to different morphotypes of Aspergillus fumigatus and Candida albicans were (1.25 ± 0.06) ％,(1.21 ± 0.03) ％,(1.22 ± 0.46) ％ and (1.26 ± 0.11) ％,while those in indirect contacting groups were (1.21 ± 0.06) ％,(1.25 ±0.04)％,(1.27 ±0.03)％ and (1.26 ±0.1)％,which were not of significant difference compared with the blank group [(1.23 ± 0.05) ％,all P ＞ 0.05].Conclusion Fungus stimuli can reduce the secretion of IFN-γ in NK cells,but have not significant influence on the secretion of IL-4.