CAJ | 학술논문

目的研制我国职业接触苯工人尿中苯巯基尿酸(SPMA)的生物限值.方法在苯作业车间选择空气中苯浓度在32.5 mg/m~3以下接苯工人55人,应用个体采样器采集空气样品,用气相色谱法检测作业者个体苯接触水平,同时采集当日工人班后尿,应用高压液相色谱/质谱法(HPLC/MS)测定尿中SPMA含量以评价苯接触者的内暴露水平,内外暴露水平的比较用相对内暴露指数(RIE)加以评定.结果接苯工人工作环境空气苯浓度范围为0.71～32.17 mg/m~3,几何平均浓度为6.98 mg/m~3,中位数为7.50 mg/m~3,尿中SPMA与个体苯暴露量存在良好的线性关系Y(μg/g Cr)=-8.625+18.367 X(mg/m~3),r=0.8035(P<0.01);将我国职业苯接触限值时间加权平均容许浓度(PC-TWA=6 mg/m~3)代入回归方程,推算工作班后尿中SPMA含量为101.58μg/g Cr.计算的RIE指数显示,接触1 mg/m~3苯可平均代谢转化为尿中SPMA 18.23 μg/g Cr,并且随接苯水平的上升其代谢转化效率呈下降趋势.结论参考国外SPMA生物限值标准,建议我国职业接触苯生物限值班后尿中SPMA为100μg/gCr(47μmol/mol Cr).
목적 연제아국직업접촉분공인뇨중분구기뇨산(SPMA)적생물한치.방법 재분작업차간선택공기중분농도재32.5 mg/m~3이하접분공인55인,응용개체채양기채집공기양품,용기상색보법검측작업자개체분접촉수평,동시채집당일공인반후뇨,응용고압액상색보/질보법(HPLC/MS)측정뇨중SPMA함량이평개분접촉자적내폭로수평,내외폭로수평적비교용상대내폭로지수(RIE)가이평정.결과 접분공인공작배경공기분농도범위위0.71～32.17 mg/m~3,궤하평균농도위6.98 mg/m~3,중위수위7.50 mg/m~3,뇨중SPMA여개체분폭로량존재량호적선성관계Y(μg/g Cr)=-8.625+18.367 X(mg/m~3),r=0.8035(P<0.01);장아국직업분접촉한치시간가권평균용허농도(PC-TWA=6 mg/m~3)대입회귀방정,추산공작반후뇨중SPMA함량위101.58μg/g Cr.계산적RIE지수현시,접촉1 mg/m~3분가평균대사전화위뇨중SPMA 18.23 μg/g Cr,병차수접분수평적상승기대사전화효솔정하강추세.결론 삼고국외SPMA생물한치표준,건의아국직업접촉분생물한치반후뇨중SPMA위100μg/gCr(47μmol/mol Cr).
Objective To establish the biological exposure limit values of urinary S-phenylmercap-turic acid (SPMA) for assessing occupational exposure to benzene. Methods Study participants were selected from 55 workers of benzene exposures below 32.5 mg/m~3. The concentration of personal exposure to benzene was measured by gas chromatography and sampled with personal sampler. The urine samples were collected at the end of work shift and individual internal exposure level was evaluated by determination of SPMA in urine by HPLC/MS method. Comparison of external and internal exposure was assessed by the relative internal expo-sure(RIE) index. Results The benzene exposure level ranged from 0.71 to 32.17 mg/m~3 (geometric mean 6.98 mg/m~3, median 7.50 mg/m~3). The urinary SPMA at the end of the work shift were significantly correlated with benzene exposure, Y (μg/g Cr)=-8.625 + 18.367X (mg/m~3), r=0.8035, (P<0.01). According to the occupational exposure limit for benzene in China and calculation of regression equation, the expected value of urinary SPMA was 101.58 μg/g Cr. Mean level of biotransformation of per mg/m~3 benzene to urinary SPMA was 18.23 μg/g Cr and the metabolic efficiencies of benzene transformation to urinary SPMA decreased with benzene exposure in-creased. Conclusion Based on abroad documents and data, biological limit value for occupational exposure to benzene in China is recommended as follows: 100 μg/g Cr (47 μmol/mol Cr) for SPMA in the urine at the end of shift.