CAJ | 학술논문

目的通过对雄性兔血清中生殖内分泌激素和阴茎组织中一氧化氮合酶(nitric oxidesynthase,eNOS)、内皮素-1(endothelin-1,ET-1)mRNA表达,探讨局部振动对雄性兔勃起功能的影响.方法将新西兰兔随机分为A组(接振强度为3.02 m/s~2)、B组(接振强度为6.13 m/s~2)、C组(接振强度为12.26 m/s~2)和1个对照组,分别在新西兰兔接振试验前和接振试验第10、20和30天,应用双抗体夹心ELISA法检测血清中睾酮、黄体生成素和雌二醇的浓度.于接振试验第30天采用荧光定量PCR法检测阴茎组织中eNOS、ET-1 mRNA表达.结果 (1)与接振试验前和对照组比较,A、B、C 3个试验组兔血清中睾酮和黄体生成素浓度随接振时间的延长和接振强度的增加呈降低趋势,C组在接振试验第30天血清中睾酮[(1.415±0.713)μg/L]明显降低,与对照组及接振前比较,差异有统计学意义(P<0.05);血清中雌二醇浓度随接振时间的延长和接振强度的增加呈增高的趋势.(2)与对照组相比较,A、B、C 3个试验组接振30 d后,阴茎组织中ET-1 mRNA[B组、C组分别为(17.39±4.59)×10~4、(36.21±13.13)×10~4拷贝数/μg]表达明显增强,eNOS mRNA[A组、B组、C组分别为(19.11±6.83)×10~4、(11.86±3.15)×10~4、(4.68±3.26)×104~4拷贝数/μg]表达明显降低.与对照组[分别为(7.58±3.07)×10~4、(34.88±10.03)×10~4拷贝数/μg]比较,差异有统计学意义(P<0.01).结论局部振动可引起血清中睾酮、黄体生成素浓度下降,雌二醇的浓度上升,局部振动通过上调阴茎组织中ET-1 mRNA和下调eNOS mRNA的表达,使组织中ET-1合成、释放增加,一氧化氮生成减少,从而影响勃起功能.
목적 통과대웅성토혈청중생식내분비격소화음경조직중일양화담합매(nitric oxidesynthase,eNOS)、내피소-1(endothelin-1,ET-1)mRNA표체,탐토국부진동대웅성토발기공능적영향.방법 장신서란토수궤분위A조(접진강도위3.02 m/s~2)、B조(접진강도위6.13 m/s~2)、C조(접진강도위12.26 m/s~2)화1개대조조,분별재신서란토접진시험전화접진시험제10、20화30천,응용쌍항체협심ELISA법검측혈청중고동、황체생성소화자이순적농도.우접진시험제30천채용형광정량PCR법검측음경조직중eNOS、ET-1 mRNA표체.결과 (1)여접진시험전화대조조비교,A、B、C 3개시험조토혈청중고동화황체생성소농도수접진시간적연장화접진강도적증가정강저추세,C조재접진시험제30천혈청중고동[(1.415±0.713)μg/L]명현강저,여대조조급접진전비교,차이유통계학의의(P<0.05);혈청중자이순농도수접진시간적연장화접진강도적증가정증고적추세.(2)여대조조상비교,A、B、C 3개시험조접진30 d후,음경조직중ET-1 mRNA[B조、C조분별위(17.39±4.59)×10~4、(36.21±13.13)×10~4고패수/μg]표체명현증강,eNOS mRNA[A조、B조、C조분별위(19.11±6.83)×10~4、(11.86±3.15)×10~4、(4.68±3.26)×104~4고패수/μg]표체명현강저.여대조조[분별위(7.58±3.07)×10~4、(34.88±10.03)×10~4고패수/μg]비교,차이유통계학의의(P<0.01).결론 국부진동가인기혈청중고동、황체생성소농도하강,자이순적농도상승,국부진동통과상조음경조직중ET-1 mRNA화하조eNOS mRNA적표체,사조직중ET-1합성、석방증가,일양화담생성감소,종이영향발기공능.
Objective To study the effects of behind legs vibrations on erectile function in male rab-bits through the concentration of plasma reproductive hormone and the expression of nitric oxide synthase (eNOS), endothelin-1 (ET-1) mRNA in vibrated male rabbits. Methods 30 male adult rabbits were assigned randomly to A group (vibration power: 3.02 m/s~2),B group(vibration power:6.13 m/s~2),C group(vibration power: 12.26 m/s~2) and control group.The concentration of expression of eNOS, ET-1 mRNA were measured with RT-PCR after rated for 30 days. Results (1) Compared with 0 days vibration,after exponred to vibration for 10,20,30 days,the A,B,C group concentration of plasma T,LH are much lower(P<0.05),the concentration of plasma E_2 is much higher. (2) Compared with control group after exposed for 30 days ,the expression of ET-1 mRNA [B group:(17.39±4.59)×10~4;C group: (36.21±13.13)×10~4] were much higher and expression of eNOS mRNA [A group: (19.11±6.83)×10~4;B group: (11.86±3.15)×10~4;C group: (4.68±3.26)×10~4] was much low-er, there were significant differences (P<0.01). Conclusions The vibration of behind legs in rabbits resulted the concentration of plasma T, LH are much lower, the concentration of plasma E_2 is much higher, increased the expression of eNOS mRNA, decreased the expression of eNOS mRNA,then vary the erectile function.