中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
2期
117-120
,共4页
逯洋%徐磊%燕贞%吴逸明%吴卫东
逯洋%徐磊%燕貞%吳逸明%吳衛東
록양%서뢰%연정%오일명%오위동
纳米氧化锌%支气管上皮细胞%白细胞介素-8%mRNA稳定性
納米氧化鋅%支氣管上皮細胞%白細胞介素-8%mRNA穩定性
납미양화자%지기관상피세포%백세포개소-8%mRNA은정성
Zinc oxide nanoparticle%Bronchial epithelial cell%Interleukin 8%mRNA stability
目的 阐明纳米氧化锌颗粒ZnO-NPs(30 nm)对人支气管上皮细胞(BEAS-2B)白细胞介素-8(IL-8)基因表达的影响及调控机制.方法 利用BEAS-2B细胞作为研究对象,采用噻唑蓝(MTT)法测定ZnO-NPs对细胞的损伤作用.应用RT-PCR技术及ELISA法检测纳米氧化锌颗粒物对细胞内IL-8 mRNA和蛋白表达的影响.利用IL-8 mRNA降解试验测定ZnO-NPs对转录后IL-8 mRNA稳定性的影响.结果 ZnO-NPs刺激可致细胞内IL-8 mRNA水平及培养液上清中IL-8蛋白含量明显升高.转录抑制剂可显著降低ZnO-NPs对IL-8 mRNA表达的诱导作用.在用放线菌素D终止IL-8 mRNA合成的前提下,ZnO-NPs可明显减缓细胞内IL-8 mRNA的降解.结论 纳米氧化锌颗粒物可提高IL-8 mRNA及蛋白在BEAS-2B内的表达水平；ZnO-NPs对细胞内IL-8 mRNA具有稳定作用.
目的 闡明納米氧化鋅顆粒ZnO-NPs(30 nm)對人支氣管上皮細胞(BEAS-2B)白細胞介素-8(IL-8)基因錶達的影響及調控機製.方法 利用BEAS-2B細胞作為研究對象,採用噻唑藍(MTT)法測定ZnO-NPs對細胞的損傷作用.應用RT-PCR技術及ELISA法檢測納米氧化鋅顆粒物對細胞內IL-8 mRNA和蛋白錶達的影響.利用IL-8 mRNA降解試驗測定ZnO-NPs對轉錄後IL-8 mRNA穩定性的影響.結果 ZnO-NPs刺激可緻細胞內IL-8 mRNA水平及培養液上清中IL-8蛋白含量明顯升高.轉錄抑製劑可顯著降低ZnO-NPs對IL-8 mRNA錶達的誘導作用.在用放線菌素D終止IL-8 mRNA閤成的前提下,ZnO-NPs可明顯減緩細胞內IL-8 mRNA的降解.結論 納米氧化鋅顆粒物可提高IL-8 mRNA及蛋白在BEAS-2B內的錶達水平；ZnO-NPs對細胞內IL-8 mRNA具有穩定作用.
목적 천명납미양화자과립ZnO-NPs(30 nm)대인지기관상피세포(BEAS-2B)백세포개소-8(IL-8)기인표체적영향급조공궤제.방법 이용BEAS-2B세포작위연구대상,채용새서람(MTT)법측정ZnO-NPs대세포적손상작용.응용RT-PCR기술급ELISA법검측납미양화자과립물대세포내IL-8 mRNA화단백표체적영향.이용IL-8 mRNA강해시험측정ZnO-NPs대전록후IL-8 mRNA은정성적영향.결과 ZnO-NPs자격가치세포내IL-8 mRNA수평급배양액상청중IL-8단백함량명현승고.전록억제제가현저강저ZnO-NPs대IL-8 mRNA표체적유도작용.재용방선균소D종지IL-8 mRNA합성적전제하,ZnO-NPs가명현감완세포내IL-8 mRNA적강해.결론 납미양화자과립물가제고IL-8 mRNA급단백재BEAS-2B내적표체수평；ZnO-NPs대세포내IL-8 mRNA구유은정작용.
Objective To clarify the effect of zinc oxide nanoparticles (ZnO-NPs) (30 nm in diameter)on the interleukin 8 (IL-8) gene expression in human bronchial epithelial cells (BEAS-2B) and its regulatory mechanism.Methods BEAS-2B cells were used in the study.The MTT assay was employed to evaluate the damage to BEAS-2B cells by ZnO-NPs.RT-PCR and ELISA were used to measure the mRNA and protein expression levels of IL-8 in the BEAS-2B cells exposed to ZnO-NPs.The IL-8 mRNA decay assay was used to determine the effect of ZnO-NPs on IL-8 mRNA stability.Results Exposure to ZnO-NPs significantly increased the level of IL-8 mRNA in BEAS-2B cells and the level of IL-8 protein in supernatant medium.The transcription inhibitor significantly reduced the mRNA expression of IL-8 induced by ZnO-NPs.ZnO-NPs significantly delayed IL-8 mRNA degradation in the BEAS-2B cells that were pretreated with actinomycin D for terminating IL-8 mRNA synthesis.Conclusion ZnO-NPs can increase the mRNA and protein expression levels of IL-8 and IL-8 mRNA stability in BEAS-2B cells.
