CAJ | 학술논문

目的研究X射线交错互补修复基因3(XRCC3)多态性与铅作业男工铅毒性易感性的关系.方法采集某蓄电池企业326名铅作业男工外周静脉血和晨尿样品,检测血铅、尿铅、血锌原卟啉(ZPP)、血钙和血铁等指标,采用聚合酶链式反应限制性片段长度多态性分析技术(PCR-RFLP)检测XRCC3基因型,分析铅作业男工不同基因型与铅毒性易感性的关系.结果 326名铅作业男工XRCC3基因分布呈现多态现象,各基因型(野生型XRCC3-241CC、杂合突变型XRCC3-241CT、纯合突变型XRCC3-241TT)的分布频率符合遗传学的Hary-Weinberg平衡定律(P＞0.05)；不同基因型组(野生型XRCC3-241CC与突变型XRCC3-241CT/TT)工人尿铅、ZPP、血钙、血铁水平比较的差异无统计学意义(P＞0.05);XRCC3-241CT/TT基因型组工人血铅水平平均值高于XRCC3-241CC基因型组,差异有统计学意义(P＜0.05)；以血铅水平1.90μmol/L为界,经x2检验、logistic回归分析显示,高铅组XRCC3-241CT/TT基因型的比例大于低铅组,差异有统计学意义(P＜0.05),XRCC3-241CT/TT基因型组工人较XRCC3-241CC基因型组工人高血铅发生率的风险明显增高(OR=2.34,95％CI=1.61～5.13)；多元线性回归显示,XRCC3-241CT/TT基因型工人血铅水平高(β=0.116,P＜0.05)、有吸烟习惯工人铅吸收明显(β=0.188,P＜0.05),良好个体防护可以减少铅吸收(β=-0.247,P＜0.05)、血清Ca2+低的个体的血铅水平高(β=-0.145,P＜0.05).结论在相同职业铅暴露环境下,XRCC3-241CT/TT基因型工人血铅水平增高,对铅毒性易感.
목적 연구X사선교착호보수복기인3(XRCC3)다태성여연작업남공연독성역감성적관계.방법 채집모축전지기업326명연작업남공외주정맥혈화신뇨양품,검측혈연、뇨연、혈자원계람(ZPP)、혈개화혈철등지표,채용취합매련식반응한제성편단장도다태성분석기술(PCR-RFLP)검측XRCC3기인형,분석연작업남공불동기인형여연독성역감성적관계.결과 326명연작업남공XRCC3기인분포정현다태현상,각기인형(야생형XRCC3-241CC、잡합돌변형XRCC3-241CT、순합돌변형XRCC3-241TT)적분포빈솔부합유전학적Hary-Weinberg평형정률(P＞0.05)；불동기인형조(야생형XRCC3-241CC여돌변형XRCC3-241CT/TT)공인뇨연、ZPP、혈개、혈철수평비교적차이무통계학의의(P＞0.05);XRCC3-241CT/TT기인형조공인혈연수평평균치고우XRCC3-241CC기인형조,차이유통계학의의(P＜0.05)；이혈연수평1.90μmol/L위계,경x2검험、logistic회귀분석현시,고연조XRCC3-241CT/TT기인형적비례대우저연조,차이유통계학의의(P＜0.05),XRCC3-241CT/TT기인형조공인교XRCC3-241CC기인형조공인고혈연발생솔적풍험명현증고(OR=2.34,95％CI=1.61～5.13)；다원선성회귀현시,XRCC3-241CT/TT기인형공인혈연수평고(β=0.116,P＜0.05)、유흡연습관공인연흡수명현(β=0.188,P＜0.05),량호개체방호가이감소연흡수(β=-0.247,P＜0.05)、혈청Ca2+저적개체적혈연수평고(β=-0.145,P＜0.05).결론 재상동직업연폭로배경하,XRCC3-241CT/TT기인형공인혈연수평증고,대연독성역감.
Objective To investigate the relationship between genetic polymorphism of X-ray repair cross-complementing gene 3 (XRCC3) and susceptibility to lead poisoning in male lead-exposed workers.Methods Peripheral venous blood and morning urine samples were collected from 326 male lead-exposed workers in a storage battery factory in Fuzhou.Blood lead,urine lead,blood zinc protoporphyrin (ZPP),blood calcium,and blood iron were measured.The genotype of XRCC3 was determined by polymerase chain reactionrestriction fragment length polymorphism method.The relationship between XRCC3 gene polymorphism and susceptibility to lead poisoning in male lead-exposed workers was analyzed.Results Genetic polymorphism of XRCC3 was seen in the 326 subjects.The frequency distribution of XRCC3 genotypes,XRCC3-241CC (wild type),XRCC3-241CT (heterozygous mutation),and XRCC3-241TT (homozygous mutation),was in accordance with the Hardy-Weinberg equilibrium (P＞0.05).There were no significant differences in urine lead,blood ZPP,blood calcium,and blood iron between the lead-exposed workers with different XRCC3 genotypes (P＞0.05).The workers with XRCC3-241CT/TT had a significantly higher mean blood lead level than those with XRCC3-241CC (P＜0.05).With a blood lead level of 1.90 μmol/L as the cutoff value,the chi-square test and logistic regression analysis showed that the proportion of workers with XRCC3-241CT/TT was significantly higher than that of workers with XRCC3-241CC in the subjects with high blood leads (P＜0.05) and that the risk of high blood lead was significantly higher in the workers with XRCC3-241CT/TT than in those with XRCC3-241CC (OR =2.34,95％ CI =1.61～5.13); the multivariate linear regression analysis showed that the workers with XRCC3-241CT/TT had high blood lead levels (β=0.116,P＜0.05),the workers with smoking habit demonstrated marked lead absorption (β=0.188,P＜0.05),good individual protection could reduce lead absorption (β=-0.247,P＜0.05),and the individuals with low serum Ca2+ levels had high blood lead levels (β=-0.145,P＜0.05).Conclusion When exposed to the same level of lead at workplace,the workers with XRCC3-241CT/TT have a significantly higher blood lead level than those with XRCC3-241CC,so the genotype of XRCC3-241CT/TT accounts for higher susceptibility to lead poisoning.