CAJ | 학술논문

目的探讨丰富环境与贫瘠环境对锰染毒小鼠学习记忆能力的影响及其作用机制.方法将40只雌性昆明小鼠随机分为对照组、正常环境染锰组(模型组)、丰富环境染锰组(丰富组)以及贫瘠环境染锰组(贫瘠组),每组10只.采用腹腔注射氯化锰的方式制造小鼠锰中毒模型,Morris水迷宫试验检测小鼠学习和记忆能力,用免疫组织化学法检测小鼠海马区CREB蛋白的表达.结果在水迷宫定位航行试验中,模型组逃避潜伏期明显高于对照组,丰富组的逃避潜伏期低于模型组,差异有统计学意义(P＜0.05),贫瘠组与模型组的差异无统计学意义(P＞0.05).空间探索实验中,丰富组的穿越平台次数则比模型组多,贫瘠组比模型组少,差异有统计学意义(P＜0.05).模型组和贫瘠组海马CA1区CREB蛋白表达量明显低于对照组,差异有统计学意义(P＜0.05)；丰富组海马CA1区CREB蛋白表达量明显比模型组高,差异有统计学意义(P＜0.05).结论丰富环境干预可改善锰中毒小鼠的学习记忆能力,增加海马内CREB的表达；丰富环境干预改善锰中毒小鼠学习记忆能力的机制可能与CREB的表达有关.
목적 탐토봉부배경여빈척배경대맹염독소서학습기억능력적영향급기작용궤제.방법 장40지자성곤명소서수궤분위대조조、정상배경염맹조(모형조)、봉부배경염맹조(봉부조)이급빈척배경염맹조(빈척조),매조10지.채용복강주사록화맹적방식제조소서맹중독모형,Morris수미궁시험검측소서학습화기억능력,용면역조직화학법검측소서해마구CREB단백적표체.결과 재수미궁정위항행시험중,모형조도피잠복기명현고우대조조,봉부조적도피잠복기저우모형조,차이유통계학의의(P＜0.05),빈척조여모형조적차이무통계학의의(P＞0.05).공간탐색실험중,봉부조적천월평태차수칙비모형조다,빈척조비모형조소,차이유통계학의의(P＜0.05).모형조화빈척조해마CA1구CREB단백표체량명현저우대조조,차이유통계학의의(P＜0.05)；봉부조해마CA1구CREB단백표체량명현비모형조고,차이유통계학의의(P＜0.05).결론 봉부배경간예가개선맹중독소서적학습기억능력,증가해마내CREB적표체；봉부배경간예개선맹중독소서학습기억능력적궤제가능여CREB적표체유관.
Objective To investigate the effects of enriched environment and impoverished environment on the learning and memory ability of manganese-exposed mice and the mechanism.Methods Forty female Kunming mice were randomly and equally divided into 4 group:control group (CG),standard environment and manganese exposure group (SEG),enriched environment and manganese exposure group (EEG),and impoverished environment and manganese exposure group (IEG).The mouse model of manganese poisoning was established by intraperitoneal injection of manganese chloride.The learning and memory ability was tested by Morris water maze.The expression of cAMP response element-binding protein (CREB) in area CA 1 of the hippocampus was measured by immunohistochemistry.Results In place navigation test,the SEG had a significantly longer escape latency than the CG (P＜0.05),and the EEG had a significantly shorter escape latency than the SEG (P＜0.05); there was no significant difference in escape latency between IEG and SEG (P＞0.05).In spatial probe test,the EEG had a significantly greater number of platform crossings than the SEG (P＜0.05),and the IEG had a significantly smaller number of platform crossings than the SEG (P＜0.05).The expression of CREB in area CA1 of the hippocampus was significantly lower in IEG and SEG than in CG (P＜0.05),and it was significantly higher in EEG than in SEG (P＜0.05).Conclusion In the enriched environment,the learning and memory ability of manganese-exposed mice can be improved,which may be due to the increased expression of CREB in the hippocampus.