中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
7期
487-491
,共5页
杨平%章军%夏勇%黄飞%徐玉英%郑一凡%杨军%朱心强
楊平%章軍%夏勇%黃飛%徐玉英%鄭一凡%楊軍%硃心彊
양평%장군%하용%황비%서옥영%정일범%양군%주심강
二氧化硅%大鼠%肺纤维化
二氧化硅%大鼠%肺纖維化
이양화규%대서%폐섬유화
Silicon dioxide%Rat%Pulmonary fibrosis
目的 探讨连续动式吸入条件下不同浓度的纳米二氧化硅(SiO2)是否可以导致大鼠肺部明显的毒性作用.方法 48只雄性SD大鼠按体重随机分成4组,包括分散剂对照组(对照组,生理盐水)和纳米SiO2低剂量组(质量浓度为0.3％)、中剂量组(质量浓度为1.0％)和高剂量组(质量浓度为3.0％).于动式染毒28 d后(每天染毒2h)处死动物,采集肺泡灌洗液(BALF)和肺组织,观察肺脏器系数、BALF中白细胞总数及分类、总蛋白含量、乳酸脱氢酶(LDH)活力、肺组织病理改变(HE染色)和肺纤维化形成情况(VG染色).结果 与对照组相比,纳米SiO2组肺脏器系数的差异无统计学意义(P＞0.05).1.0％和3.0％纳米SiO2组BALF中的白细胞总数明显高于对照组,差异有统计学意义(P＜0.05).各剂量纳米SiO2组的总蛋白含量和LDH活力与对照组比较,差异均无统计学意义(P＞0.05).与对照组相比,各剂量纳米SiO2组淋巴细胞的比例明显降低,单核和巨噬细胞的比例明显升高,差异有统计学意义(P＜0.05).HE染色显示,纳米SiO2组肺泡壁明显增厚,支气管和血管壁周围发生明显的炎性细胞浸润,各纳米SiO2组肺纤维化VG染色均未见胶原纤维分布的明显增加.结论 本实验条件下,连续28d动式吸入纳米SiO2只引起大鼠肺部显著的炎症反应,未出现肺纤维化现象.
目的 探討連續動式吸入條件下不同濃度的納米二氧化硅(SiO2)是否可以導緻大鼠肺部明顯的毒性作用.方法 48隻雄性SD大鼠按體重隨機分成4組,包括分散劑對照組(對照組,生理鹽水)和納米SiO2低劑量組(質量濃度為0.3％)、中劑量組(質量濃度為1.0％)和高劑量組(質量濃度為3.0％).于動式染毒28 d後(每天染毒2h)處死動物,採集肺泡灌洗液(BALF)和肺組織,觀察肺髒器繫數、BALF中白細胞總數及分類、總蛋白含量、乳痠脫氫酶(LDH)活力、肺組織病理改變(HE染色)和肺纖維化形成情況(VG染色).結果 與對照組相比,納米SiO2組肺髒器繫數的差異無統計學意義(P＞0.05).1.0％和3.0％納米SiO2組BALF中的白細胞總數明顯高于對照組,差異有統計學意義(P＜0.05).各劑量納米SiO2組的總蛋白含量和LDH活力與對照組比較,差異均無統計學意義(P＞0.05).與對照組相比,各劑量納米SiO2組淋巴細胞的比例明顯降低,單覈和巨噬細胞的比例明顯升高,差異有統計學意義(P＜0.05).HE染色顯示,納米SiO2組肺泡壁明顯增厚,支氣管和血管壁週圍髮生明顯的炎性細胞浸潤,各納米SiO2組肺纖維化VG染色均未見膠原纖維分佈的明顯增加.結論 本實驗條件下,連續28d動式吸入納米SiO2隻引起大鼠肺部顯著的炎癥反應,未齣現肺纖維化現象.
목적 탐토련속동식흡입조건하불동농도적납미이양화규(SiO2)시부가이도치대서폐부명현적독성작용.방법 48지웅성SD대서안체중수궤분성4조,포괄분산제대조조(대조조,생리염수)화납미SiO2저제량조(질량농도위0.3％)、중제량조(질량농도위1.0％)화고제량조(질량농도위3.0％).우동식염독28 d후(매천염독2h)처사동물,채집폐포관세액(BALF)화폐조직,관찰폐장기계수、BALF중백세포총수급분류、총단백함량、유산탈경매(LDH)활력、폐조직병리개변(HE염색)화폐섬유화형성정황(VG염색).결과 여대조조상비,납미SiO2조폐장기계수적차이무통계학의의(P＞0.05).1.0％화3.0％납미SiO2조BALF중적백세포총수명현고우대조조,차이유통계학의의(P＜0.05).각제량납미SiO2조적총단백함량화LDH활력여대조조비교,차이균무통계학의의(P＞0.05).여대조조상비,각제량납미SiO2조림파세포적비례명현강저,단핵화거서세포적비례명현승고,차이유통계학의의(P＜0.05).HE염색현시,납미SiO2조폐포벽명현증후,지기관화혈관벽주위발생명현적염성세포침윤,각납미SiO2조폐섬유화VG염색균미견효원섬유분포적명현증가.결론 본실험조건하,련속28d동식흡입납미SiO2지인기대서폐부현저적염증반응,미출현폐섬유화현상.
Objective To investigate the pulmonary toxicity of different concentrations of nano-silica (nano-SiO2) under continuous dynamic inhalation conditions in the rat.Methods 48 male Sprague-Dawley rats were randomly divided into four groups,including the dispersant control group (saline) and nano-SiO2 lowdose group (0.3％,w/v),the middle-dose group (1％) and the high-dose group (3％).Animals were sacrificed at 28 d after exposure under continuous dynamic inhalation conditions,and bronchoalveolar lavage fluid (BALF) and lung tissue were collected.And following items were observed:body coefficient,BALF related items (leukocytes and classification,total protein content,LDH activity),lung tissue pathological changes (HE staining),and pulmonary fibrosis forming (collagen fiber VG staining).Results Compared to the dispersant control group,there was no significant change on lung organ coefficient in Nano-SiO2 group (P＞0.05).The BALF total WBC count in 1％ and 3％ in nano-SiO2 groups showed higher value than the dispersant control group (P＜0.05).No obvious changes were found on total protein content and LDH activity in nano-SiO2 groups compared to the dispersant control group (P＞0.05).For differential WBC counts,lymphocyte count in BALF in nano-SiO2 groups was significantly decreased (P＜0.05),monocyte and macrophage counts were significantly increased (P＜0.05),but there was no difference on the proportion of neutrophils (P＞0.05).HE staining results showed that the obvious thickening of alveolar wall in nano-SiO2 groups,inflammatory cell infiltration also increased around the bronchial and vascular wall.Lung fibrosis VG staining showed no significant change of collagen fiber distribution.Conclusion Under our experimental conditions,the continuous dynamic inhalation of nano-SiO2 only caused the significant inflammation in rat lungs,pulmonary fibrosis phenomenon could not be observed significantly.
