中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
7期
496-499
,共4页
张放%门金龙%邵华%张志虎%冯斌
張放%門金龍%邵華%張誌虎%馮斌
장방%문금룡%소화%장지호%풍빈
苯乙烯%环氧水解酶类%多态性,单核苷酸%代谢
苯乙烯%環氧水解酶類%多態性,單覈苷痠%代謝
분을희%배양수해매류%다태성,단핵감산%대사
Styrene%Epoxide Hydrolase%Polymorphisms,Single nucleticle%Metabolism
目的 探讨环氧化物水解酶1(epoxide hydrolase 1,EPHX1)基因多态性对苯乙烯体内代谢的影响.方法 选择山东省某玻璃钢游艇生产企业喷漆车间工龄在1年以上,防护情况基本相同的全部工人(56人)作为研究对象.分别测定个体苯乙烯8h时间加权平均浓度(8 h-TWA)、尿中代谢产物-苯乙醇酸(mandelic acid,MA)和苯乙醛酸(phenyl glyoxylic acid,PGA)浓度,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测研究对象的EPHX1基因多态性.结果 工人尿中MA浓度为(177.25±82.36) mg/g Cr,PGA浓度为(145.91±69.73) mg/g Cr,苯乙烯8 h-TWA为(133.28±95.81) mg/m3.尿中MA及PGA浓度与苯乙烯8 h-TWA均呈正相关关系(R=0.861,P＜0.05; R=0.868,P＜0.05).按苯乙烯8 h-TWA＞50 mg/m3为高暴露组,8 h-TWA≤50 mg/m3为低暴露组,将研究对象分为高暴露组和低暴露组,高暴露组和低暴露组受试者携带高活力EPHXI基因型个体尿中的MA和PGA浓度明显高于携带EPHX1低活力组基因型者,差异有统计学意义(P＜0.05).结论 EPHXI基因多态性对苯乙烯在体内的代谢过程可能有一定影响.
目的 探討環氧化物水解酶1(epoxide hydrolase 1,EPHX1)基因多態性對苯乙烯體內代謝的影響.方法 選擇山東省某玻璃鋼遊艇生產企業噴漆車間工齡在1年以上,防護情況基本相同的全部工人(56人)作為研究對象.分彆測定箇體苯乙烯8h時間加權平均濃度(8 h-TWA)、尿中代謝產物-苯乙醇痠(mandelic acid,MA)和苯乙醛痠(phenyl glyoxylic acid,PGA)濃度,採用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)法檢測研究對象的EPHX1基因多態性.結果 工人尿中MA濃度為(177.25±82.36) mg/g Cr,PGA濃度為(145.91±69.73) mg/g Cr,苯乙烯8 h-TWA為(133.28±95.81) mg/m3.尿中MA及PGA濃度與苯乙烯8 h-TWA均呈正相關關繫(R=0.861,P＜0.05; R=0.868,P＜0.05).按苯乙烯8 h-TWA＞50 mg/m3為高暴露組,8 h-TWA≤50 mg/m3為低暴露組,將研究對象分為高暴露組和低暴露組,高暴露組和低暴露組受試者攜帶高活力EPHXI基因型箇體尿中的MA和PGA濃度明顯高于攜帶EPHX1低活力組基因型者,差異有統計學意義(P＜0.05).結論 EPHXI基因多態性對苯乙烯在體內的代謝過程可能有一定影響.
목적 탐토배양화물수해매1(epoxide hydrolase 1,EPHX1)기인다태성대분을희체내대사적영향.방법 선택산동성모파리강유정생산기업분칠차간공령재1년이상,방호정황기본상동적전부공인(56인)작위연구대상.분별측정개체분을희8h시간가권평균농도(8 h-TWA)、뇨중대사산물-분을순산(mandelic acid,MA)화분을철산(phenyl glyoxylic acid,PGA)농도,채용취합매련반응-한제성편단장도다태성(PCR-RFLP)법검측연구대상적EPHX1기인다태성.결과 공인뇨중MA농도위(177.25±82.36) mg/g Cr,PGA농도위(145.91±69.73) mg/g Cr,분을희8 h-TWA위(133.28±95.81) mg/m3.뇨중MA급PGA농도여분을희8 h-TWA균정정상관관계(R=0.861,P＜0.05; R=0.868,P＜0.05).안분을희8 h-TWA＞50 mg/m3위고폭로조,8 h-TWA≤50 mg/m3위저폭로조,장연구대상분위고폭로조화저폭로조,고폭로조화저폭로조수시자휴대고활력EPHXI기인형개체뇨중적MA화PGA농도명현고우휴대EPHX1저활력조기인형자,차이유통계학의의(P＜0.05).결론 EPHXI기인다태성대분을희재체내적대사과정가능유일정영향.
Objective To investigate the role of genetic polymorphisms of epoxide hydrolase 1 (EPHX1) in the metabolism of styrene in vivo.Methods Fifty-six styrene-exposed workers,who worked in the painting workshop of an enterprise for manufacturing glass fiber-reinforced plastic yachts in Shandong Province,China for over one year and were protected in approximately the same way,were selected as study subjects.The 8-hour time-weighted average concentration (8 h-TWA) of styrene and the concentrations of mandelic acid (MA) and phenyl glyoxylic acid (PGA) as urinary metabolites were measured.The genetic polymorphisms of EPHX1 were detected by polymerase chain reaction-restriction fragment length polymorphism analysis.Results The urinary concentrations of MA and PGA were 177.25±82.36 mg/g Cr and 145.91t69.73 mg/g Cr,respectively,and the 8 h-TWA of styrene was 133.28±95.81 mg/m3.Urinary concentrations of MA and PGA were positively correlated with 8 h-TWA of styrene (R=0.861,P＜0.05; R=0.868,P＜0.05).The subjects were divided into high-exposure group (8 h-TWA ＞50 mg/m3) and low-exposure group (8 h-TWA ≤50 mg/m3),and in the two groups,the urinary concentrations of MA and PGA were significantly higher in the individuals carrying high-activity genotypes of EPHX1 than in those carrying low-activity genotypes of EPHX1 (P＜0.05).Conclusion Genetic polymorphisms ofEPHX 1 play an important role in the metabolic process of styrene in vivo.
