CAJ | 학술논문

目的应用细胞因子抗体芯片(cytokine antibody chip,CAC)分析尘肺患者血清中细胞因子表达的变化.方法采用CAC技术平台,测定12例尘肺患者和3例正常对照血清中60种细胞因子.结果在尘肺患者血清中筛选到白细胞介素(IL-1α、-1β、-2、-4～16)、集落刺激因子(M-CSF)、干扰素(IFN-γ)、肿瘤坏死因子(TNF-α和TNF-β)、人骨形成蛋白6(BMP-6)、角蛋白细胞生长因子(FGF-7)、神经营养因子3(NT-3)和干细胞因子(SCF)等高表达的细胞因子,重组人(I-309)、单核细胞趋化蛋白(MCP-1、MCP-2、MCP-3、MCP-4)、巨噬细胞炎性蛋白(MIP-1-δ、MIP-3-α)等在尘肺患者血清中低表达的细胞因子.结论尘肺患者血清中绝大部分细胞因子表达存在差异.
목적 응용세포인자항체심편(cytokine antibody chip,CAC)분석진폐환자혈청중세포인자표체적변화.방법 채용CAC기술평태,측정12례진폐환자화3례정상대조혈청중60충세포인자.결과 재진폐환자혈청중사선도백세포개소(IL-1α、-1β、-2、-4～16)、집락자격인자(M-CSF)、간우소(IFN-γ)、종류배사인자(TNF-α화TNF-β)、인골형성단백6(BMP-6)、각단백세포생장인자(FGF-7)、신경영양인자3(NT-3)화간세포인자(SCF)등고표체적세포인자,중조인(I-309)、단핵세포추화단백(MCP-1、MCP-2、MCP-3、MCP-4)、거서세포염성단백(MIP-1-δ、MIP-3-α)등재진폐환자혈청중저표체적세포인자.결론 진폐환자혈청중절대부분세포인자표체존재차이.
Objective To investigate the changes in expression of serum cytokines in patients with pneumoconiosis using cytokine antibody chips (CACs).Methods The CAC technology was applied to measure the serum levels of 60 cytokines in 12 patients with pneumoconiosis and 3 normal controls.Results In the patients with pneumoconiosis,the highly expressed serum cytokines included interleukin (IL)-1α,IL-1β,IL-2,ILs 4-16,macrophage colony-stimulating factor,interferon-γ,tumor necrosis factor (TNF)-α,TNF-β,human bone morphogenetic protein-6,fibroblast growth factor-7,neurotrophin-3,and stem cell factor,and the lowly expressed serum cytokines included recombinant human I-309,monocyte chemoattractant protein (MCP) -1,MCP-2,MCP-3,MCP-4,macrophage inflammatory protein (MIP)-1-δ,and MIP-3-α.Conclusion Patients with pneumoconiosis have changes in the expression of most serum cytokines.