中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
8期
585-588
,共4页
张明%王延让%杨德一%王倩%刘义涛%刘晓婷%齐丽杰
張明%王延讓%楊德一%王倩%劉義濤%劉曉婷%齊麗傑
장명%왕연양%양덕일%왕천%류의도%류효정%제려걸
乙苯%大鼠%肾毒性%HO-1%氧化应激
乙苯%大鼠%腎毒性%HO-1%氧化應激
을분%대서%신독성%HO-1%양화응격
Ethylbenzene%Rat%Renal toxicity%heme oxygenase-1%Oxidative Stress
目的 观察乙苯对大鼠肾组织血红素氧化酶(HO)-1表达的影响.方法 选择雄性SD大鼠40只,随机分为4组:对照组、低剂量组、中剂量组和高剂量组,每组10只,分别暴露于0、433.5mg/m3(100 ppm)、4335.0 mg/m3(1000 ppm)和6500.0 mg/m3(1500 ppm)的乙苯,6 h/d,5d/周,共13周,建立大鼠乙苯亚慢性染毒模型；检测大鼠肾组织谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力变化,Real-time PCR和Western blot检测肾组织HO-1在转录和翻译水平的表达情况.结果 与对照组相比,各剂量组大鼠肾组织GSH-Px、CAT活力均下降,中、高剂量乙苯染毒组大鼠肾组织SOD活力均降低,差异均有统计学意义(P＜0.05)；各剂量组HO-1基因表达水平明显高于对照组,高剂量组HO-1表达水平明显高于低、中剂量组,中、高剂量组HO-1蛋白表达水平高于对照组和低剂量组,差异有统计学意义(P＜0.05),高剂量组大鼠HO-1表达水平均明显高于低、中剂量组,差异有统计学意义(P＜0.05).结论 一定剂量的乙苯可诱导大鼠肾组织细胞内HO-1表达水平升高,抗氧化水平降低,导致氧化应激损伤.
目的 觀察乙苯對大鼠腎組織血紅素氧化酶(HO)-1錶達的影響.方法 選擇雄性SD大鼠40隻,隨機分為4組:對照組、低劑量組、中劑量組和高劑量組,每組10隻,分彆暴露于0、433.5mg/m3(100 ppm)、4335.0 mg/m3(1000 ppm)和6500.0 mg/m3(1500 ppm)的乙苯,6 h/d,5d/週,共13週,建立大鼠乙苯亞慢性染毒模型；檢測大鼠腎組織穀胱甘肽過氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、過氧化氫酶(CAT)活力變化,Real-time PCR和Western blot檢測腎組織HO-1在轉錄和翻譯水平的錶達情況.結果 與對照組相比,各劑量組大鼠腎組織GSH-Px、CAT活力均下降,中、高劑量乙苯染毒組大鼠腎組織SOD活力均降低,差異均有統計學意義(P＜0.05)；各劑量組HO-1基因錶達水平明顯高于對照組,高劑量組HO-1錶達水平明顯高于低、中劑量組,中、高劑量組HO-1蛋白錶達水平高于對照組和低劑量組,差異有統計學意義(P＜0.05),高劑量組大鼠HO-1錶達水平均明顯高于低、中劑量組,差異有統計學意義(P＜0.05).結論 一定劑量的乙苯可誘導大鼠腎組織細胞內HO-1錶達水平升高,抗氧化水平降低,導緻氧化應激損傷.
목적 관찰을분대대서신조직혈홍소양화매(HO)-1표체적영향.방법 선택웅성SD대서40지,수궤분위4조:대조조、저제량조、중제량조화고제량조,매조10지,분별폭로우0、433.5mg/m3(100 ppm)、4335.0 mg/m3(1000 ppm)화6500.0 mg/m3(1500 ppm)적을분,6 h/d,5d/주,공13주,건립대서을분아만성염독모형；검측대서신조직곡광감태과양화물매(GSH-Px)、초양화물기화매(SOD)、과양화경매(CAT)활력변화,Real-time PCR화Western blot검측신조직HO-1재전록화번역수평적표체정황.결과 여대조조상비,각제량조대서신조직GSH-Px、CAT활력균하강,중、고제량을분염독조대서신조직SOD활력균강저,차이균유통계학의의(P＜0.05)；각제량조HO-1기인표체수평명현고우대조조,고제량조HO-1표체수평명현고우저、중제량조,중、고제량조HO-1단백표체수평고우대조조화저제량조,차이유통계학의의(P＜0.05),고제량조대서HO-1표체수평균명현고우저、중제량조,차이유통계학의의(P＜0.05).결론 일정제량적을분가유도대서신조직세포내HO-1표체수평승고,항양화수평강저,도치양화응격손상.
Objective To investigate the effect of ethylbenzene on the expression of heme oxygenase-1(HO-1) in rat renal tissues.Methods Forty male Sprague-Dawley rats were randomly and equally allocated to control group,low-dose exposure group,moderate-dose exposure group,and high-dose exposure group to inhale different doses of ethylbenzene (0,433.5 mg/m3 (100 ppm),4335.0 mg/m3 (1000 ppm),and 6500.0 mg/m3 (1500 ppm))for 6 h per day,5 days per week,for 13 weeks.After the rat model of subchronic ethylbenzene exposure was established,the activities of glutathione peroxidase (GSH-Px),superoxide dismutase (SOD),and catalase (CAT) in renal tissues were measured,and the mRNA and protein expression levels of HO-1 in renal tissues were measured by real-time PCR and Western blot.Results Compared with the control group,all exposure groups showed significantly decreased activities of GSH-Px and CAT in renal tissues and the moderate-and high-dose exposure groups showed significantly decreased activity of SOD in renal tissues (P＜0.05).All exposure groups showed significantly higher expression of HO-1 than the control group (P＜0.05).The high-dose exposure group showed significantly higher expression of HO-1 than the low-and moderate-dose exposure group (P＜0.05),and the moderate-and high-dose exposure group had significantly higher expression of HO-1 than the control group and low-dose exposure group (P＜0.05).Conclusion A certain dose of ethylbenzene can induce elevated expression of HO-1 and decreased antioxidant levels in rat renal tissues,thus leading to oxidative stress damage.
