中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
8期
611-614
,共4页
吡咯加合物%2,5-己二酮%血清
吡咯加閤物%2,5-己二酮%血清
필각가합물%2,5-기이동%혈청
Pyrrole adducts%2,5-hexanedione%Serum
目的 探讨吡咯加合物的生成与2,5-己二酮(2,5-hexanedione,2,5-HD)浓度之间的关系,为正己烷毒性研究提供实验依据.方法 混合正常人离体血清,过滤除菌后配制2,5-HD终浓度为200、100、50、25、10 mg/L及空白血清,37℃培养,不同时间点取血清,比色法测定血清中吡咯加合物的生成量,气相色谱法测定血清中2,5-HD剩余量.结果 血清中吡咯加合物的含量随培养时间的延长而上升,且与2,5-HD存在剂量依赖关系,实验结束时吡咯加合物的含量在各浓度组间的差异有统计学意义(P＜0.05)；血清中2,5-HD的含量随培养时间的延长而降低,实验结束时按2,5-HD浓度由高到低,2,5-HD浓度分别下降29％、55％、22％、44％和40％；2,5-HD含量的减少量与吡咯加合物的增加量之间存在正相关,按2,5-HD浓度由高到低,相关系数r分别为0.865、0.697、0.835、0.823和0.814.结论 随2,5-HD浓度增加,吡咯加合物生成量增加；血清中2,5-HD减少量与吡咯加合物的增加量呈正相关.
目的 探討吡咯加閤物的生成與2,5-己二酮(2,5-hexanedione,2,5-HD)濃度之間的關繫,為正己烷毒性研究提供實驗依據.方法 混閤正常人離體血清,過濾除菌後配製2,5-HD終濃度為200、100、50、25、10 mg/L及空白血清,37℃培養,不同時間點取血清,比色法測定血清中吡咯加閤物的生成量,氣相色譜法測定血清中2,5-HD剩餘量.結果 血清中吡咯加閤物的含量隨培養時間的延長而上升,且與2,5-HD存在劑量依賴關繫,實驗結束時吡咯加閤物的含量在各濃度組間的差異有統計學意義(P＜0.05)；血清中2,5-HD的含量隨培養時間的延長而降低,實驗結束時按2,5-HD濃度由高到低,2,5-HD濃度分彆下降29％、55％、22％、44％和40％；2,5-HD含量的減少量與吡咯加閤物的增加量之間存在正相關,按2,5-HD濃度由高到低,相關繫數r分彆為0.865、0.697、0.835、0.823和0.814.結論 隨2,5-HD濃度增加,吡咯加閤物生成量增加；血清中2,5-HD減少量與吡咯加閤物的增加量呈正相關.
목적 탐토필각가합물적생성여2,5-기이동(2,5-hexanedione,2,5-HD)농도지간적관계,위정기완독성연구제공실험의거.방법 혼합정상인리체혈청,과려제균후배제2,5-HD종농도위200、100、50、25、10 mg/L급공백혈청,37℃배양,불동시간점취혈청,비색법측정혈청중필각가합물적생성량,기상색보법측정혈청중2,5-HD잉여량.결과 혈청중필각가합물적함량수배양시간적연장이상승,차여2,5-HD존재제량의뢰관계,실험결속시필각가합물적함량재각농도조간적차이유통계학의의(P＜0.05)；혈청중2,5-HD적함량수배양시간적연장이강저,실험결속시안2,5-HD농도유고도저,2,5-HD농도분별하강29％、55％、22％、44％화40％；2,5-HD함량적감소량여필각가합물적증가량지간존재정상관,안2,5-HD농도유고도저,상관계수r분별위0.865、0.697、0.835、0.823화0.814.결론 수2,5-HD농도증가,필각가합물생성량증가；혈청중2,5-HD감소량여필각가합물적증가량정정상관.
Objective To investigate the relationship between formation of pyrrole adducts and concentration of 2,5-hexanedione (2,5-HD) and to provide an experimental basis for the study on toxicity of nhexane.Methods Serum samples were collected from normal persons and were then filtered and sterilized.They were mixed with 2,5-HD to obtain sera with final 2,5-HD concentrations of 10,25,50,100,and 200 mg/L,and blank serum was also prepared.The sera were cultured at 37℃ and taken at different time points.Colorimetry was used to quantify the pyrrole adducts formed in sera,and gas chromatography was used to measure the remaining 2,5-HD levels in sera.Results The content of pyrrole adducts increased as the culture proceeded and was dependent on the dose of 2,5-HD; at the end of the experiment,the content of pyrrole adducts differed significantly across all concentration groups (P＜0.5).The concentrations of 2,5-HD decreased as the culture proceeded; at the end of the experiment,the concentrations of 2,5-HD,from the highest to the lowest,decreased by 29％,55％,22％,44％,and 40％,respectively.The decrease in 2,5-HD had a positive correlation with the increase in pyrrole adducts,and the correlation coefficients for 200～10 mg/L 2,5-HD were 0.865,0.697,0.835,0.823,and 0.814,respectively.Conclusion The content of formed pyrrole adducts increases as the concentration of 2,5-HD rises; there is a positive correlation between the decrease in 2,5-HD and the increase in pyrrole adducts in human serum.
