中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
9期
690-692
,共3页
宋明芬%王玉文%王翀%彭开良
宋明芬%王玉文%王翀%彭開良
송명분%왕옥문%왕충%팽개량
纳米氧化铜%遗传毒性%氧化应激
納米氧化銅%遺傳毒性%氧化應激
납미양화동%유전독성%양화응격
copper oxide nanoparticles%genetoxicity%oxidative stress
目的 研究纳米氧化铜对小鼠的遗传毒性及氧化应激水平.方法 30只小鼠随机分成对照组、50、150 mg/kg纳米氯化铜染毒组,染毒组分别一次性腹腔注射50、150 mg/kg纳米氧化铜,对照组给予含0.05％ Tween 80相同体积的生理盐水.于染毒前及染毒后24、48及72 h分别测定尾静脉外周血网织红细胞微核发生率和尿中8-羟基-脱氧鸟嘌呤核苷(8-OH-dG)水平.染毒72 h,处死小鼠,取肝、肾和股骨骨髓,分别提取DNA并测定DNA中8-OH-dG.结果 50 mg/kg染毒组48 h,150 mg/kg组24和48 h外周血网织红细胞微核发生率(3.1 1‰±1.46、4.25‰±0.43‰、5.42‰±0.76‰)比两组染毒前(1.55‰±0.39‰、1.11‰±0.19‰)及相应的对照组(1.55‰±0.28‰、1.00±0.67‰)明显升高,差异有统计学意义(P＜0.05或P＜0.01).与染毒前及对照组比较,各时间50、150 mg/kg染毒组尿8-OH-dG水平(ng/mg尿肌酐)均明显升高,差异有统计学意义(P＜0.05或P＜0.01).50和150 mg/kg组肝组织DNA中8-OH-dG水平(4.53±1.27、7.69±2.78)明显高于对照组(0.85±0.14),差异有统计学意义(P＜0.01).结论 纳米氧化铜对小鼠具有遗传毒性,并致其氧化应激水平升高.
目的 研究納米氧化銅對小鼠的遺傳毒性及氧化應激水平.方法 30隻小鼠隨機分成對照組、50、150 mg/kg納米氯化銅染毒組,染毒組分彆一次性腹腔註射50、150 mg/kg納米氧化銅,對照組給予含0.05％ Tween 80相同體積的生理鹽水.于染毒前及染毒後24、48及72 h分彆測定尾靜脈外週血網織紅細胞微覈髮生率和尿中8-羥基-脫氧鳥嘌呤覈苷(8-OH-dG)水平.染毒72 h,處死小鼠,取肝、腎和股骨骨髓,分彆提取DNA併測定DNA中8-OH-dG.結果 50 mg/kg染毒組48 h,150 mg/kg組24和48 h外週血網織紅細胞微覈髮生率(3.1 1‰±1.46、4.25‰±0.43‰、5.42‰±0.76‰)比兩組染毒前(1.55‰±0.39‰、1.11‰±0.19‰)及相應的對照組(1.55‰±0.28‰、1.00±0.67‰)明顯升高,差異有統計學意義(P＜0.05或P＜0.01).與染毒前及對照組比較,各時間50、150 mg/kg染毒組尿8-OH-dG水平(ng/mg尿肌酐)均明顯升高,差異有統計學意義(P＜0.05或P＜0.01).50和150 mg/kg組肝組織DNA中8-OH-dG水平(4.53±1.27、7.69±2.78)明顯高于對照組(0.85±0.14),差異有統計學意義(P＜0.01).結論 納米氧化銅對小鼠具有遺傳毒性,併緻其氧化應激水平升高.
목적 연구납미양화동대소서적유전독성급양화응격수평.방법 30지소서수궤분성대조조、50、150 mg/kg납미록화동염독조,염독조분별일차성복강주사50、150 mg/kg납미양화동,대조조급여함0.05％ Tween 80상동체적적생리염수.우염독전급염독후24、48급72 h분별측정미정맥외주혈망직홍세포미핵발생솔화뇨중8-간기-탈양조표령핵감(8-OH-dG)수평.염독72 h,처사소서,취간、신화고골골수,분별제취DNA병측정DNA중8-OH-dG.결과 50 mg/kg염독조48 h,150 mg/kg조24화48 h외주혈망직홍세포미핵발생솔(3.1 1‰±1.46、4.25‰±0.43‰、5.42‰±0.76‰)비량조염독전(1.55‰±0.39‰、1.11‰±0.19‰)급상응적대조조(1.55‰±0.28‰、1.00±0.67‰)명현승고,차이유통계학의의(P＜0.05혹P＜0.01).여염독전급대조조비교,각시간50、150 mg/kg염독조뇨8-OH-dG수평(ng/mg뇨기항)균명현승고,차이유통계학의의(P＜0.05혹P＜0.01).50화150 mg/kg조간조직DNA중8-OH-dG수평(4.53±1.27、7.69±2.78)명현고우대조조(0.85±0.14),차이유통계학의의(P＜0.01).결론 납미양화동대소서구유유전독성,병치기양화응격수평승고.
Objective To investigate the genotoxicity and oxidative stress induced by copper oxide nanoparticles in mice.Methods Thirty mice were randomly divided into control group and low-and high-dose exposure groups.The low-and high-dose exposure groups were given copper oxide nanoparticles (50 and 150 mg/kg) by a single intraperitoneal injection,while the control group was given an equal volume of normal saline containing 0.05％ Tween 80.The micronucleus rate of reticulocytes in peripheral blood from the caudal vein and urinary 8-hydroxy-deoxyguanosine (8-OH-dG) level were measured before and at 24,48,and 72 h after exposure.All the mice were sacrificed at 72 h after exposure,the liver,kidney,and femoral marrow were taken for DNA extraction,and 8-OH-dG in DNA was quantified.Results The micronucleus rates of peripheral blood reticulocytes in low-dose exposure group at 48 h (3.11±1.46‰) and in high-dose exposure group at 24 and 48 h (4.25±0.43‰ and 5.42±0.76‰) were significantly increased compared with those before exposure (1.55±0.3‰and 1.11±0.19‰) and those in control group (1.55±0.28‰ and 1.00±0.67‰) (P＜0.05 or P＜0.01).The urinary 8-OH-dG levels (ng/mg creatinine) in low-and high-dose exposure groups at all time points were significantly increased compared with those before exposure and those in control group (P＜0.05 or P＜0.01).The low-and high-dose exposure groups had significantly higher content of 8-OH-dG in liver DNA than the control group (4.53±1.27 and 7.69±2.78 vs 0.85±0.14,P＜0.01).Conclusion Copper oxide nanoparticles cause genotoxicity and increase oxidative stress in mice.
