CAJ | 학술논문

目的探讨染矽尘大鼠早期支气管肺泡灌洗液(BALF)中炎性细胞因子的变化.方法将实验大鼠随机分为对照组、15、30、60 mg/ml染矽尘实验组,每组42只.一次性气管内注入染尘,不同处理后1、3、7、14、21、28 d每组分别处死7只大鼠,收集BALF,采用ELISA法测定肿瘤坏死因子(tumor necrosis factor,TNF)、白细胞介素(interleukin,IL)-1、IL-6、IL-16、巨噬细胞炎性蛋白-1(macrophageinflammatory protein-1,MIP-I)、单核细胞趋化因子-1(monocyte chemoattractant protein-1,MCP-1)、肿瘤坏死因子(tumor necrosis factor,TNF-α)、转化生长因子(transforming growth factor-β,TGF-β)的浓度.结果各剂量组BALF中炎性细胞因子在各时间点较对照组均有不同程度的增高,在染尘初期(1～3 d时)BALF中IL-1随染尘剂量的增加而增高的趋势较明显,14d时IL-6、IL-16随染尘剂量增加而增高的趋势较明显;染尘组IL-1、IL-6、IL-16均在14d时出现浓度峰值.不同剂量染尘组MIP-1α、MCP-1浓度的差异有统计学意义(FMIP-1α=30.106,P＜0.01;FMCP-1=17.193,P＜0.01).各组不同染尘时间MCP-1浓度的差异无统计学意义(F=0.618,P＞0.05);在1～14d BALF中TNF-α随接尘剂量的增加而增高,剂量反应关系明显,差异有统计学意义(P＜0.05).各剂量组TNF-α在14d出现高峰.高剂量组14、21、28d BALF中TGF-β水平均高于低剂量组,14、28 d BALF中TGF-β水平均明显高于中剂量组,差异均有统计学意义(P＜0.05).结论 IL-1、IL-6、IL-16、MIP-1α、MCP-1、TNF-α在矽肺炎症发生发展过程中发挥了作用,TGF-β可能与纤维化过程有关联.
목적 탐토염석진대서조기지기관폐포관세액(BALF)중염성세포인자적변화.방법 장실험대서수궤분위대조조、15、30、60 mg/ml염석진실험조,매조42지.일차성기관내주입염진,불동처리후1、3、7、14、21、28 d매조분별처사7지대서,수집BALF,채용ELISA법측정종류배사인자(tumor necrosis factor,TNF)、백세포개소(interleukin,IL)-1、IL-6、IL-16、거서세포염성단백-1(macrophageinflammatory protein-1,MIP-I)、단핵세포추화인자-1(monocyte chemoattractant protein-1,MCP-1)、종류배사인자(tumor necrosis factor,TNF-α)、전화생장인자(transforming growth factor-β,TGF-β)적농도.결과 각제량조BALF중염성세포인자재각시간점교대조조균유불동정도적증고,재염진초기(1～3 d시)BALF중IL-1수염진제량적증가이증고적추세교명현,14d시IL-6、IL-16수염진제량증가이증고적추세교명현;염진조IL-1、IL-6、IL-16균재14d시출현농도봉치.불동제량염진조MIP-1α、MCP-1농도적차이유통계학의의(FMIP-1α=30.106,P＜0.01;FMCP-1=17.193,P＜0.01).각조불동염진시간MCP-1농도적차이무통계학의의(F=0.618,P＞0.05);재1～14d BALF중TNF-α수접진제량적증가이증고,제량반응관계명현,차이유통계학의의(P＜0.05).각제량조TNF-α재14d출현고봉.고제량조14、21、28d BALF중TGF-β수평균고우저제량조,14、28 d BALF중TGF-β수평균명현고우중제량조,차이균유통계학의의(P＜0.05).결론 IL-1、IL-6、IL-16、MIP-1α、MCP-1、TNF-α재석폐염증발생발전과정중발휘료작용,TGF-β가능여섬유화과정유관련.
Objective To investigate the changes in the levels of inflammatory cytokines in bronchoalveolar lavage fluid (BALF) in rats exposed to silica dust.Methods Experimental rats were randomly divided into control group and three experimental groups (doses of dust:15,30,and 60 mg/ml),with 42 rats in each group.Each rat in the control group was treated with 1 ml of normal saline by intratracheal instillation,while each rat in the experimental groups was exposed to 1 ml of silica suspension by a single intratracheal instillation.Seven rats in each group were killed at 1,3,7,14,21,and 28 days after exposure,and then BALF was collected.Enzyme-linked immunosorbent assay was used to measure the levels of interleukin (IL)-1,IL-6,IL-16,macrophage inflammatory protein-1 alpha (MIP-1α),monocyte chemoattractant protein-1 (MCP-1),tumor necrosis factor-α (TNF-α),and transforming growth factor-β (TGF-β).Results The levels of cytokines in each experimental group were higher than those in the control group at any time point.In the early stage of exposure (day 1-3),BALF IL-1 level increased significantly with the increase in dust dose,and on day 14,BALF IL-6 and IL-16 levels increased significantly with the increase in dust dose; the levels of IL-1,IL-6,and IL-16 in the experimental groups reached the peak on day 14.There were significant differences in the levels of MIP-1α and MCP-1 between the experimental groups (FMIP-1α=30.106,P＜0.01; FMCP-1=17.193,P＜0.01).In each group,the level of MCP-1 varied significantly at different time points (F=0.618,P＞0.05).On day 1-14,BALF TNF-α level increased with the increase in dust dose,with a significant dose-response relationship (P＜0.05).In each experimental group,TNF-α level reached the peak on day 14.On days 14,21,and 28,the highdose group had significantly higher BALF TGF-β levels than the low-dose group (P＜0.05); on days 14 and 28,the high-dose group had significantly higher BALF TGF-β levels than the middle-dose group (P＜0.05).Conclusion IL-1,IL-6,IL-16,MIP-1α,MCP-1,and TNF-α play a role in the development and progression of silicosis inflammation.TGF-β may be related to (related to; associated with; correlated with) fibrosis.