中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
11期
806-810
,共5页
袁华%王清华%王韵阳%谢长明%谢克勤%赵秀兰
袁華%王清華%王韻暘%謝長明%謝剋勤%趙秀蘭
원화%왕청화%왕운양%사장명%사극근%조수란
1-溴丙烷%二十二碳六烯酸类%神经酸%神经行为学表现
1-溴丙烷%二十二碳六烯痠類%神經痠%神經行為學錶現
1-추병완%이십이탄륙희산류%신경산%신경행위학표현
1-Bromopropane%Docosahexaenoic acid%Nervonic acid%Neurobehavioral manifestations
目的 观察二十二碳六烯酸(docosahexaenoic acid,DHA)和神经酸(nervonic acid)对1-溴丙烷(1-bromopropane,1-BP)致大鼠学习认知功能损伤的保护作用及机制.方法 SPF级雄性Wistar大鼠40只,随机分为溶剂对照组、1-BP染毒组(800 mg/kg)、神经酸(150 mg/kg)+ 1-BP组(800 mg/kg)、DHA(500mg/kg)+1-BP组(800 mg/kg),每组10只.分别经灌胃给予相应受试物7d,第8天开始采用Morris水迷宫试验检测大鼠的学习和记忆能力,连续5d.水迷宫结束次日断头处死大鼠,迅速剥离大鼠大脑皮层,在冰浴中制备组织匀浆,分别测定还原型谷胱甘肽(glutathione,GSH)和丙二醛(malondialdehyde,MDA)含量、谷胱甘肽还原酶(glutathione reductase,GR)及γ-谷氨酰半胱氨酸连接酶(γ-glutamate cysteineligase,γ-GCL)活力.结果 Morris水迷宫定位航行试验中,1-BP染毒组大鼠的逃避潜伏期和游泳总路程明显高于对照组,差异有统计学意义(P＜0.05);与1-BP染毒组比较,DHA+1-BP组大鼠的逃避潜伏期和游泳总路程明显降低,差异有统计学意义(P＜0.05).空间探索试验中,与1-BP组比较,DHA+1-BP组和神经酸+1-BP组的穿越平台次数明显增加,差异均有统计学意义(P＜0.05).与对照组比较,1-BP染毒组平台周边时间/总时间比值明显减少,与1-BP组比较,DHA+1-BP组动物的平台周边时间/总时间比值明显增加,差异均有统计学意义(P＜0.05).与对照组比较,1-BP染毒组动物大脑GSH含量降低了18.1％,DHA+1-BP组GSH含量明显高于1-BP组,差异均有统计学意义(P＜0.01).与1-BP染毒组比较,DHA+1-BP组和神经酸+1-BP组MDA含量明显降低,DHA+1-BP组GR酶活力明显增加,神经酸+1-BP组γ-GCL酶活力明显升高,差异均有统计学意义(P＜0.05).结论 1-BP染毒导致大鼠大脑氧化应激,损伤认知功能;DHA和神经酸在一定程度上可减轻1-BP对大鼠认知功能的损伤;增强GR和γ-GCL酶活力,升高大脑GSH含量,可能是其作用机制之一.
目的 觀察二十二碳六烯痠(docosahexaenoic acid,DHA)和神經痠(nervonic acid)對1-溴丙烷(1-bromopropane,1-BP)緻大鼠學習認知功能損傷的保護作用及機製.方法 SPF級雄性Wistar大鼠40隻,隨機分為溶劑對照組、1-BP染毒組(800 mg/kg)、神經痠(150 mg/kg)+ 1-BP組(800 mg/kg)、DHA(500mg/kg)+1-BP組(800 mg/kg),每組10隻.分彆經灌胃給予相應受試物7d,第8天開始採用Morris水迷宮試驗檢測大鼠的學習和記憶能力,連續5d.水迷宮結束次日斷頭處死大鼠,迅速剝離大鼠大腦皮層,在冰浴中製備組織勻漿,分彆測定還原型穀胱甘肽(glutathione,GSH)和丙二醛(malondialdehyde,MDA)含量、穀胱甘肽還原酶(glutathione reductase,GR)及γ-穀氨酰半胱氨痠連接酶(γ-glutamate cysteineligase,γ-GCL)活力.結果 Morris水迷宮定位航行試驗中,1-BP染毒組大鼠的逃避潛伏期和遊泳總路程明顯高于對照組,差異有統計學意義(P＜0.05);與1-BP染毒組比較,DHA+1-BP組大鼠的逃避潛伏期和遊泳總路程明顯降低,差異有統計學意義(P＜0.05).空間探索試驗中,與1-BP組比較,DHA+1-BP組和神經痠+1-BP組的穿越平檯次數明顯增加,差異均有統計學意義(P＜0.05).與對照組比較,1-BP染毒組平檯週邊時間/總時間比值明顯減少,與1-BP組比較,DHA+1-BP組動物的平檯週邊時間/總時間比值明顯增加,差異均有統計學意義(P＜0.05).與對照組比較,1-BP染毒組動物大腦GSH含量降低瞭18.1％,DHA+1-BP組GSH含量明顯高于1-BP組,差異均有統計學意義(P＜0.01).與1-BP染毒組比較,DHA+1-BP組和神經痠+1-BP組MDA含量明顯降低,DHA+1-BP組GR酶活力明顯增加,神經痠+1-BP組γ-GCL酶活力明顯升高,差異均有統計學意義(P＜0.05).結論 1-BP染毒導緻大鼠大腦氧化應激,損傷認知功能;DHA和神經痠在一定程度上可減輕1-BP對大鼠認知功能的損傷;增彊GR和γ-GCL酶活力,升高大腦GSH含量,可能是其作用機製之一.
목적 관찰이십이탄륙희산(docosahexaenoic acid,DHA)화신경산(nervonic acid)대1-추병완(1-bromopropane,1-BP)치대서학습인지공능손상적보호작용급궤제.방법 SPF급웅성Wistar대서40지,수궤분위용제대조조、1-BP염독조(800 mg/kg)、신경산(150 mg/kg)+ 1-BP조(800 mg/kg)、DHA(500mg/kg)+1-BP조(800 mg/kg),매조10지.분별경관위급여상응수시물7d,제8천개시채용Morris수미궁시험검측대서적학습화기억능력,련속5d.수미궁결속차일단두처사대서,신속박리대서대뇌피층,재빙욕중제비조직균장,분별측정환원형곡광감태(glutathione,GSH)화병이철(malondialdehyde,MDA)함량、곡광감태환원매(glutathione reductase,GR)급γ-곡안선반광안산련접매(γ-glutamate cysteineligase,γ-GCL)활력.결과 Morris수미궁정위항행시험중,1-BP염독조대서적도피잠복기화유영총로정명현고우대조조,차이유통계학의의(P＜0.05);여1-BP염독조비교,DHA+1-BP조대서적도피잠복기화유영총로정명현강저,차이유통계학의의(P＜0.05).공간탐색시험중,여1-BP조비교,DHA+1-BP조화신경산+1-BP조적천월평태차수명현증가,차이균유통계학의의(P＜0.05).여대조조비교,1-BP염독조평태주변시간/총시간비치명현감소,여1-BP조비교,DHA+1-BP조동물적평태주변시간/총시간비치명현증가,차이균유통계학의의(P＜0.05).여대조조비교,1-BP염독조동물대뇌GSH함량강저료18.1％,DHA+1-BP조GSH함량명현고우1-BP조,차이균유통계학의의(P＜0.01).여1-BP염독조비교,DHA+1-BP조화신경산+1-BP조MDA함량명현강저,DHA+1-BP조GR매활력명현증가,신경산+1-BP조γ-GCL매활력명현승고,차이균유통계학의의(P＜0.05).결론 1-BP염독도치대서대뇌양화응격,손상인지공능;DHA화신경산재일정정도상가감경1-BP대대서인지공능적손상;증강GR화γ-GCL매활력,승고대뇌GSH함량,가능시기작용궤제지일.
Objective To investigate the protective effects ofdocosahexaenoic acid (DHA) and nervonic acid (NA) on the learning and memory abilities in rats exposed to 1-bromopropane (1-BP) and their action mechanisms.Methods Forty male Wister rats (specific pathogen-free) were randomly divided into 4 groups (n=10 for each),i.e.,solvent control group,1-BP (800 mg/kg) group,NA (150 mg/kg)+1-BP (800 mg/kg) group,and DHA (500 mg/kg)+1-BP (800 mg/kg) group.The rats were given respective test substances by gavage for 7 d.The Morris water maze (MWM) test was performed from days 8 to 12 to evaluate the rats' learning and memory abilities.After MWM test,rats were sacrificed in the next day,and cerebral cortex was quickly dissected and homogenized in an ice bath.The supernatant of the obtained homogenate was collected to measure the content of glutathione (GSH) and malondialdehyde (MDA) and the activities of glutathione reductase (GR) and γ-glutamate cysteine ligase (γ-GCL).Results The MWM spatial navigation test showed that the 1-BP group had significantly longer escape latency and significantly longer total swimming distance compared with the control group (P＜0.05),while the DHA+1-BP group had significant decreases in escape latency and total swimming distance compared with the 1-BP group (P＜0.05).The spatial probe test showed that the number of platform crossings was significantly greater in the DHA+1-BP group and NA+1-BP group than in the 1-BP group (P＜0.05); compared with the control group,the 1-BP group had a significantly lower ratio of time spent in the zone around the platform to total time (P＜0.05),and the ratio was significantly higher in the DHA+1-BP group than in the 1-BP group (P＜0.05).Compared with the control group,the 1-BP group had a 18.1％ decrease in GSH content,and DHA could significantly reverse 1-BP-induced decrease in GSH content (P＜0.05).Compared with the 1-BP group,the DHA+1-BP group and NA+1-BP group had significantly decreased MDA content (P＜0.05),the DHA+1-BP group had significantly increased GR activity (P＜0.05),and the NA+1-BP group had significantly increased γ-GCL activity (P＜0.05).Conclusion The rats exposed to 1-BP have oxidative stress in the brain and impaired cognitive function.DHA and NA can reduce 1-BP-induced cognitive function impairment in rats,possibly by increasing the activities of GR and γ-GCL and the content of GSH in the brain.
