中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
11期
811-814
,共4页
吴德生%杨淋清%黄遂%刘建军%徐新云%黄海燕%龚春梅%胡恭华%刘庆成
吳德生%楊淋清%黃遂%劉建軍%徐新雲%黃海燕%龔春梅%鬍恭華%劉慶成
오덕생%양림청%황수%류건군%서신운%황해연%공춘매%호공화%류경성
三氯乙烯%精子%线粒体膜电位
三氯乙烯%精子%線粒體膜電位
삼록을희%정자%선립체막전위
trichloroethylene%sperm%mitochondria membrane potential
目的 研究三氯乙烯体外染毒对SD大鼠精子的损伤.方法 0、2、4、6、8、10 mmol/L的三氯乙烯对SD大鼠附睾精子体外染毒后4h收获细胞,吉姆萨染色法观察精子的形态变化,流式细胞仪检测精子线粒体膜电位的改变.结果 6、8、10 mmol/L三氯乙烯剂量组精子运动能力明显降低,与对照组比较,差异有统计学意义(P＜0.01).8、10 mmol/L三氯乙烯剂量组精子形态总畸变率明显高于对照组,差异有统计学意义(P＜0.01).随染毒剂量的增高,精子细胞线粒体膜电位下降的比例升高,4、6、8、10 mmol/L三氯乙烯染毒剂量组精子细胞凋亡率与对照组的差异均有统计学意义(P＜0.01).结论 三氯乙烯体外染毒可导致雄性SD大鼠精子细胞运动能力下降,精子畸形率升高,凋亡率增加.
目的 研究三氯乙烯體外染毒對SD大鼠精子的損傷.方法 0、2、4、6、8、10 mmol/L的三氯乙烯對SD大鼠附睪精子體外染毒後4h收穫細胞,吉姆薩染色法觀察精子的形態變化,流式細胞儀檢測精子線粒體膜電位的改變.結果 6、8、10 mmol/L三氯乙烯劑量組精子運動能力明顯降低,與對照組比較,差異有統計學意義(P＜0.01).8、10 mmol/L三氯乙烯劑量組精子形態總畸變率明顯高于對照組,差異有統計學意義(P＜0.01).隨染毒劑量的增高,精子細胞線粒體膜電位下降的比例升高,4、6、8、10 mmol/L三氯乙烯染毒劑量組精子細胞凋亡率與對照組的差異均有統計學意義(P＜0.01).結論 三氯乙烯體外染毒可導緻雄性SD大鼠精子細胞運動能力下降,精子畸形率升高,凋亡率增加.
목적 연구삼록을희체외염독대SD대서정자적손상.방법 0、2、4、6、8、10 mmol/L적삼록을희대SD대서부고정자체외염독후4h수획세포,길모살염색법관찰정자적형태변화,류식세포의검측정자선립체막전위적개변.결과 6、8、10 mmol/L삼록을희제량조정자운동능력명현강저,여대조조비교,차이유통계학의의(P＜0.01).8、10 mmol/L삼록을희제량조정자형태총기변솔명현고우대조조,차이유통계학의의(P＜0.01).수염독제량적증고,정자세포선립체막전위하강적비례승고,4、6、8、10 mmol/L삼록을희염독제량조정자세포조망솔여대조조적차이균유통계학의의(P＜0.01).결론 삼록을희체외염독가도치웅성SD대서정자세포운동능력하강,정자기형솔승고,조망솔증가.
Objective To study in vitro sperm damage caused by trichloroethylene in male rats.Methods Sperms ofSprague-Dawley (SD) rats were collected 4 hours after being contaminated by trichloroethylene of 0,2,4,6,8,and 10 mmol/L in vitro.Giemsa staining was performed to observe the morphological changes of sperms,and flow cytometer was used to detect the changes in mitochondrial membrane potential.Results The sperm motilities in 6,8,and 10 mmol/L trichloroethylene groups decreased significantly compared with that in control group (P＜0.01); the sperm aberration rates in 8 and 10 mmol/L trichloroethylene groups were significantly higher than that in control group (P＜0.01).With the increase in exposure dose,the proportion of sperms with reduced mitochondrial membrane potential increased,and there were significant differences in sperm apoptosis rate between the 4,6,8,and 10 mmol/L trichloroethylene groups and control group (P＜0.01).Conclusion In vitro exposure to trichloroethylene can reduce sperm motility and increase the aberration rate and apoptosis rate of sperms in male SD rats.
