中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
11期
830-833
,共4页
韩卫南%邵华%陈学磊%韩贵芝%孟潇
韓衛南%邵華%陳學磊%韓貴芝%孟瀟
한위남%소화%진학뢰%한귀지%맹소
苯乙烯%谷胱甘肽转移酶%基因多态性
苯乙烯%穀胱甘肽轉移酶%基因多態性
분을희%곡광감태전이매%기인다태성
Styrene%Gultathione transferases%Gene%Polymorphism,single nucleotide
目的 探讨GSTP1和GSTM1及GSTT1基因多态性与苯乙烯接触工人尿中苯乙烯巯基尿酸(PHEMA)水平的关系.方法 选取山东省某机车外壳生产厂苯乙烯作业人员126名为接触组,非苯乙烯作业人员150名为对照组.应用限制性片段长度多态性聚合酶链式反应(PCR-RFLP)法检测个体GSTP1基因多态性,多重PCR法检测GSTM1和GSTT1的基因多态性,分析GSTP1、GSTM1、GSTT1基因多态性与苯乙烯接触工人尿中PHEMA水平的关系.结果 本次调查中3个基因型分布符合中国人群分布特征.在高浓度苯乙烯的接触水平下,携带GSTP1 (exon5,A105G)野生基因型AA者尿中PHEMA浓度(43.58 mg/g)明显高于携带突变基因型AG和GG者(AG:29.769 mg/g; GG:30.245mg/g),携带GSTM1野生型基因型者尿中PHEMA浓度(40.197 mg/g)明显高于携带缺失型基因型者(28.866 mg/g),差异有统计学意义(P＜0.05).未发现GSTT1野生型与缺失型的个体有明显差异.对照组中3个基因多态性与个体尿中PHEMA的水平关系不明显.结论 GSTP1和GSTM1基因多态性可能与个体尿中PHEMA水平有关系.
目的 探討GSTP1和GSTM1及GSTT1基因多態性與苯乙烯接觸工人尿中苯乙烯巰基尿痠(PHEMA)水平的關繫.方法 選取山東省某機車外殼生產廠苯乙烯作業人員126名為接觸組,非苯乙烯作業人員150名為對照組.應用限製性片段長度多態性聚閤酶鏈式反應(PCR-RFLP)法檢測箇體GSTP1基因多態性,多重PCR法檢測GSTM1和GSTT1的基因多態性,分析GSTP1、GSTM1、GSTT1基因多態性與苯乙烯接觸工人尿中PHEMA水平的關繫.結果 本次調查中3箇基因型分佈符閤中國人群分佈特徵.在高濃度苯乙烯的接觸水平下,攜帶GSTP1 (exon5,A105G)野生基因型AA者尿中PHEMA濃度(43.58 mg/g)明顯高于攜帶突變基因型AG和GG者(AG:29.769 mg/g; GG:30.245mg/g),攜帶GSTM1野生型基因型者尿中PHEMA濃度(40.197 mg/g)明顯高于攜帶缺失型基因型者(28.866 mg/g),差異有統計學意義(P＜0.05).未髮現GSTT1野生型與缺失型的箇體有明顯差異.對照組中3箇基因多態性與箇體尿中PHEMA的水平關繫不明顯.結論 GSTP1和GSTM1基因多態性可能與箇體尿中PHEMA水平有關繫.
목적 탐토GSTP1화GSTM1급GSTT1기인다태성여분을희접촉공인뇨중분을희구기뇨산(PHEMA)수평적관계.방법 선취산동성모궤차외각생산엄분을희작업인원126명위접촉조,비분을희작업인원150명위대조조.응용한제성편단장도다태성취합매련식반응(PCR-RFLP)법검측개체GSTP1기인다태성,다중PCR법검측GSTM1화GSTT1적기인다태성,분석GSTP1、GSTM1、GSTT1기인다태성여분을희접촉공인뇨중PHEMA수평적관계.결과 본차조사중3개기인형분포부합중국인군분포특정.재고농도분을희적접촉수평하,휴대GSTP1 (exon5,A105G)야생기인형AA자뇨중PHEMA농도(43.58 mg/g)명현고우휴대돌변기인형AG화GG자(AG:29.769 mg/g; GG:30.245mg/g),휴대GSTM1야생형기인형자뇨중PHEMA농도(40.197 mg/g)명현고우휴대결실형기인형자(28.866 mg/g),차이유통계학의의(P＜0.05).미발현GSTT1야생형여결실형적개체유명현차이.대조조중3개기인다태성여개체뇨중PHEMA적수평관계불명현.결론 GSTP1화GSTM1기인다태성가능여개체뇨중PHEMA수평유관계.
Objective To investigate the relationship between genetic polymorphisms of glutathione S-transferase P1 (GSTP1),glutathione S-transferase M1 (GSTM1),and glutathione S-transferase T1 (GSTT1) and urinary level of mercapturic acids of styrene (PHEMAs) in workers exposed to styrene.Methods One hundred and twenty-six workers exposed to styrene were selected as exposure group,and 150 workers without styrene exposure as the control group; all the workers came from a locomotive shell production factory in Shandong Province,China.The PCR-RFLP technique was applied to analyze the individual genetic polymorphisms of GSTP1; the multiplex PCR technique was used to investigate the genetic polymorphisms of GSTM1 and GSTT1; the relationship between genetic polymorphisms of GSTP1,GSTM1,and GSTT1 and urinary level of PHEMAs in workers exposed to styrene was statistically analyzed.Results The three genotypes investigated in the study had a distribution in accordance with the Chinese population.With exposure to highconcentration styrene,the individuals carrying GSTP1 (exon 5,A 105G) AA genotype (wild type) had a significantly higher urinary level of PHEMAs (43.58 mg/g) than those with mutant genotypes AG (29.769 mg/g) and GG (30.245 mg/g); the urinary level of PHEMAs in individuals carrying wild-type GSTM1 genotype was significantly higher than that in individuals carrying deficient-type GSTM1 genotype (40.197 mg/g vs 28.866 mg/g,P＜0.05); no significant difference in urinary level of PHEMAs was found between individuals carrying wild-type GSTT1 genotype and deficient-type GSTT1 genotype.There was no significant relationship between the three gene polymorphisms and urinary level of PHEMAs in the control group.Conclusion The genetic polymorphisms of GSTP1 and GSTM 1 may be related to urinary level of PHEMAs in workers exposed to styrene.
