CAJ | 학술논문

目的探讨吸烟对尘肺患者微小RNAs(micro RNAs,miRNAs)表达的影响.方法采用RT-qPCR方法对36例非吸烟尘肺患者和38例吸烟尘肺患者的miR-21、miR-200c、miR-16、miR-204、miR-206、miR-155、let-7g、miR-30b、miR-192表达水平进行检测,用两独立样本t检验检验两组表达的差异.结果吸烟尘肺患者与非吸烟尘肺患者血清中miR-192的表达比较,差异有统计学意义(P＜0.05),并且在吸烟组Ⅰ、Ⅱ期尘肺中渐进性的降低;其中miR-16在尘肺患者血清中的表达量最高,而miR-204尘肺患者血清中的表达量最低.结论尘肺患者血清不同miRNAs的表达是差异的,吸烟对尘肺患者miRNAs表达产生影响.
목적 탐토흡연대진폐환자미소RNAs(micro RNAs,miRNAs)표체적영향.방법 채용RT-qPCR방법대36례비흡연진폐환자화38례흡연진폐환자적miR-21、miR-200c、miR-16、miR-204、miR-206、miR-155、let-7g、miR-30b、miR-192표체수평진행검측,용량독립양본t검험검험량조표체적차이.결과 흡연진폐환자여비흡연진폐환자혈청중miR-192적표체비교,차이유통계학의의(P＜0.05),병차재흡연조Ⅰ、Ⅱ기진폐중점진성적강저;기중miR-16재진폐환자혈청중적표체량최고,이miR-204진폐환자혈청중적표체량최저.결론 진폐환자혈청불동miRNAs적표체시차이적,흡연대진폐환자miRNAs표체산생영향.
Objective To investigate the effect of smoking on the microRNAs (miRNAs) expression in pneumoconiosis patients.Methods Real-time qPCR was used to measure the expression levels of miR-21,miR-200c,miR-16,miR-204,miR-206,miR-155,let-7g,miR-30b,and miR-192 in 36 non-smoking patients with pneumoconiosis and 38 smoking patients with pneumoconiosis,and the differences in expression levels between the two groups were evaluated by two-independent samples t-test.Results The expression of miR-192 in serum showed a significant difference between non-smoking and smoking pneumoconiosis patients (P＜0.05),and it decreased gradually in smoking patients with stage Ⅰ and Ⅱ pneumoconiosis.In the serum of all pneumoconiosis patients,the expression level of miR-16 was the highest,while the expression level of miR-204 was the lowest.Conclusion Pneumoconiosis patients have differential expression of miRNAs in serum,and smoking has an effect on the miRNAs expression in pneumoconiosis patients.