中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2012年
10期
895-898
,共4页
热量限制%胰岛素分泌细胞
熱量限製%胰島素分泌細胞
열량한제%이도소분비세포
Caloric restriction%Insulin-secreting cells
目的 观察热卡限制对大鼠成年后胰岛β细胞质量的影响. 方法 将16 只 8周龄雄性SD大鼠随机分为对照组7只和热卡限制组9只,对照组大鼠自由进食,热卡限制组大鼠以对照组摄食量的70％喂养,共干预24周.血脂测定指标为三酰甘油(TG)、胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C);应用免疫组化法测定β细胞团块质量;应用ELISA法测定胰腺组织匀浆中超氧化物歧化酶(SOD)活力及丙二醛(MDA)水平. 结果 热卡限制24周后,热卡限制组大鼠较对照组体质量增长(45 g比184 g,P＜0.01)、TG水平[(0.61±0.15) mmol/L比(0.78±0.14)mmol/L,P＜0.05]明显降低;血胰腺β细胞团块质量[(43.6±9.8) mg比(31.9±11.6)mg,P＜0.05]、每毫克胰腺β细胞团块质量[(89.7±7.4)μg/mg比(44.8±14.1)μg/mg,P＜0.01]以及单位体质量的β细胞团块质量[(11.5±2.5)×10-5比(6.3±2.3)×10-5,P＜0.01]则明显升高.两组大鼠胰腺组织匀浆中的总SOD活力分别为[(0.91±0.30) nmol/ mg protein比(0.68±0.14)nmol/ mg protein],MDA水平分别为[4.97±0.65)U/mg protein.比(6.05±2.14)U/mg protein],组间差异无统计学意义(P＞0.05). 结论 幼年期开始热卡限制有助于提高大鼠成年后的腴岛β细胞团块质量,这可能是热卡限制改善大鼠在糖负荷后早期胰岛素分泌相的重要原因之一.
目的 觀察熱卡限製對大鼠成年後胰島β細胞質量的影響. 方法 將16 隻 8週齡雄性SD大鼠隨機分為對照組7隻和熱卡限製組9隻,對照組大鼠自由進食,熱卡限製組大鼠以對照組攝食量的70％餵養,共榦預24週.血脂測定指標為三酰甘油(TG)、膽固醇(TC)、高密度脂蛋白膽固醇(HDL-C)、低密度脂蛋白膽固醇(LDL-C);應用免疫組化法測定β細胞糰塊質量;應用ELISA法測定胰腺組織勻漿中超氧化物歧化酶(SOD)活力及丙二醛(MDA)水平. 結果 熱卡限製24週後,熱卡限製組大鼠較對照組體質量增長(45 g比184 g,P＜0.01)、TG水平[(0.61±0.15) mmol/L比(0.78±0.14)mmol/L,P＜0.05]明顯降低;血胰腺β細胞糰塊質量[(43.6±9.8) mg比(31.9±11.6)mg,P＜0.05]、每毫剋胰腺β細胞糰塊質量[(89.7±7.4)μg/mg比(44.8±14.1)μg/mg,P＜0.01]以及單位體質量的β細胞糰塊質量[(11.5±2.5)×10-5比(6.3±2.3)×10-5,P＜0.01]則明顯升高.兩組大鼠胰腺組織勻漿中的總SOD活力分彆為[(0.91±0.30) nmol/ mg protein比(0.68±0.14)nmol/ mg protein],MDA水平分彆為[4.97±0.65)U/mg protein.比(6.05±2.14)U/mg protein],組間差異無統計學意義(P＞0.05). 結論 幼年期開始熱卡限製有助于提高大鼠成年後的腴島β細胞糰塊質量,這可能是熱卡限製改善大鼠在糖負荷後早期胰島素分泌相的重要原因之一.
목적 관찰열잡한제대대서성년후이도β세포질량적영향. 방법 장16 지 8주령웅성SD대서수궤분위대조조7지화열잡한제조9지,대조조대서자유진식,열잡한제조대서이대조조섭식량적70％위양,공간예24주.혈지측정지표위삼선감유(TG)、담고순(TC)、고밀도지단백담고순(HDL-C)、저밀도지단백담고순(LDL-C);응용면역조화법측정β세포단괴질량;응용ELISA법측정이선조직균장중초양화물기화매(SOD)활력급병이철(MDA)수평. 결과 열잡한제24주후,열잡한제조대서교대조조체질량증장(45 g비184 g,P＜0.01)、TG수평[(0.61±0.15) mmol/L비(0.78±0.14)mmol/L,P＜0.05]명현강저;혈이선β세포단괴질량[(43.6±9.8) mg비(31.9±11.6)mg,P＜0.05]、매호극이선β세포단괴질량[(89.7±7.4)μg/mg비(44.8±14.1)μg/mg,P＜0.01]이급단위체질량적β세포단괴질량[(11.5±2.5)×10-5비(6.3±2.3)×10-5,P＜0.01]칙명현승고.량조대서이선조직균장중적총SOD활력분별위[(0.91±0.30) nmol/ mg protein비(0.68±0.14)nmol/ mg protein],MDA수평분별위[4.97±0.65)U/mg protein.비(6.05±2.14)U/mg protein],조간차이무통계학의의(P＞0.05). 결론 유년기개시열잡한제유조우제고대서성년후적유도β세포단괴질량,저가능시열잡한제개선대서재당부하후조기이도소분비상적중요원인지일.
Objective To study the effect of calorie restriction at early age of rats on their islet β cell mass in adulthood.Methods Sixteen 8-week-old male SD rats were randomized to control group (n=7) and calorie restricted group (n =9).The rats in control group took food freely,while the ones in calorie restricted group were given 70％ calorie of the control group.After 24 week calorie restriction,cholesterol (TC),triglyceride (TG),high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were tested.The β cell mass was measured by immunohistochefistry and the activity of superoxide dismutase (SOD) as well as the level of malondialdehyde (MDA) in pancreas homogenate were determined by ELISA.Results The increase of the body weights(45 g vs.184 g)and the level of TG [(0.61±0.15)mmol/L vs.(0.78±0.14)mmol/ L]of the rats in the calorie restricted group were lower than those of the control group (P＜0.05),while the β cell mass [(43.6±9.8)mg vs.(31.9± 11.6)mg],β cell mass of every milligram pancreas tissue[(89.7 ± 7.4) μg/mg vs.(44.8g ± 14.1) μg/mg] and β cell mass per body weight[(11.5±2.5) × 10-5 vs.(6.3 ±2.3) × 10-5]of the rats in the calorie restricted group were higher than those of the control group (P＜0.05).There were no differences in the SOD activity [(0.91±0.30)nmol/ mg protein vs.(0.68±0.14)nmol/ mg protein]and MDA level [4.97± 0.65)U /mg protein vs.(6.05 ±2.14)U/mg protein] in pancreas homogenate between the two groups (P＞0.05).Conclusions Calorie restriction at early age of rats may increase the islet β cell mass in their adulthood.
