CAJ | 학술논문

目的通过裸鼠肺内和胸膜腔移植人肺腺癌A549细胞的方法建立晚期肺癌胸水的动物模型. 方法突变系SPF级Balb/c-nu雌性裸鼠分为3组,每组30只,分别于皮下、肺内、肺内及胸膜腔移植肺腺癌A549细胞,观察小鼠的生存时间、体质量、饲料消耗、恶病质状况、肺癌生长和胸水形成的情况. 结果裸鼠中位数生存时间肺内及胸膜腔移植、肺内移植均较皮下移植明显缩短,皮下移植生存期范围离散程度明显高于肺内、肺内及胸膜腔移植两组;肺内、肺内及胸膜腔移植两组平均生存时间无差异,平均有效生存时间有差异;肺内、肺内及胸膜腔移植两组小鼠摄食减少、体质量下降和恶病质症状和体征较皮下移植明显,且肺内及胸膜腔移植比肺内移植更多見.4周内肺内及胸膜腔移植胸水发生率93.3％,而肺内移植仅20.0％出现胸水. 结论人肺腺癌A549细胞裸鼠肺内和胸膜腔同期移植模型可作为晚期肺癌胸水的动物模型.
목적 통과라서폐내화흉막강이식인폐선암A549세포적방법건립만기폐암흉수적동물모형. 방법 돌변계SPF급Balb/c-nu자성라서분위3조,매조30지,분별우피하、폐내、폐내급흉막강이식폐선암A549세포,관찰소서적생존시간、체질량、사료소모、악병질상황、폐암생장화흉수형성적정황. 결과 라서중위수생존시간폐내급흉막강이식、폐내이식균교피하이식명현축단,피하이식생존기범위리산정도명현고우폐내、폐내급흉막강이식량조;폐내、폐내급흉막강이식량조평균생존시간무차이,평균유효생존시간유차이;폐내、폐내급흉막강이식량조소서섭식감소、체질량하강화악병질증상화체정교피하이식명현,차폐내급흉막강이식비폐내이식경다견.4주내폐내급흉막강이식흉수발생솔93.3％,이폐내이식부20.0％출현흉수. 결론 인폐선암A549세포라서폐내화흉막강동기이식모형가작위만기폐암흉수적동물모형.
Objective To establish the advanced lung cancer model with effusion in the pleural cavity by implanting the lung adenocarcinoma A549 cells into the lung and pleural cavity of mice.Methods The Balb /c-nu female mice were divided into three groups and injected with the subculturing lung adenocarcinoma A549 cells to make three models in the subcutaneous (group A),the lungs (group B) and the lungs and pleural cavities (group c).The survival time,weightconsume of feeds,signs and symptoms of cachexia,growth of lung cancer and pleural effusion were observed.Results The median survival time in group C and group B were obviously reduced compared with group A.The range discrete in the group A was higher than group B and group C.There was no significant difference between group B and group C in the average survival time.The effective survival time was significant different between group B and group C.There were differences among three groups in the signs and symptoms of cachexia,the weight and consume of feeds.In 4 weeks,the rate of growing effusion in the pleural cavity was 93.3％ in group C,and only 20.0％ in group B.Conclusions The mice modal implanted the lung adenocarcinoma A549 cells into the lung aud pleural cavity of mice in the same time can be as the adwanced lung cancer model with effusion in the pleural cavity.