中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2012年
11期
1027-1030
,共4页
肖迎春%刘楠%周宜灿%陈荣华
肖迎春%劉楠%週宜燦%陳榮華
초영춘%류남%주의찬%진영화
痴呆,血管性%大鼠,Wistar%记忆障碍
癡呆,血管性%大鼠,Wistar%記憶障礙
치태,혈관성%대서,Wistar%기억장애
Dementia%vascular%Rats,Wistar%Memory disorders
目的 研究文拉法辛对血管性痴呆(VD)模型大鼠学习记忆功能的影响及机制. 方法 将Wistar大鼠随机分为假手术组、模型组和治疗组,各30只.模型组和治疗组采用改良Pulsinellis四血管阻断法建立血管性痴呆大鼠模型.造模后第3天开始假手术组及模型组予蒸馏水灌胃,治疗组予文拉法辛灌胃,剂量15 mg/kg,共28 d.采用单程逃避实验测试各组大鼠的主动学习记忆能力,并观察各组大鼠海马及前额叶皮质5羟色胺(5-HT)、去甲肾上腺素(NE)含量及认脑源性神经营养因子(BDNF)含量变化. 结果 造模后30 d与假手术组大鼠单程逃避实验完成逃避的百分比数(92.3±5.6)相比,模型组完成逃避的百分比数(50.3±6.2)明显减少(P＜0.01),模型组大鼠海马及前额叶皮质5-HT、NE含量明显下降,分别为(226±34) pg/g和(340±40) pg/g,(601±66)pg/g和(657±43) pg/g(P＜0.01),BDNF含量升高,海马和前额叶皮质中吸光度分别为(0.50±0.04)和(0.49±0.04) (P＜0.05).与模型组相比,治疗组大鼠单程逃避实验完成逃避的百分比数明显增加至(62.2±4.6)(P＜0.05),大鼠海马及前额叶皮质5-HT、NE含量升高,分别为(264±45)pg/g和(379±42)pg/g,(798±51)pg/g和(798±51) pg/g(P＜0.05),BDNF含量进一步升高,海马和前额叶皮质中吸光度分别为(0.58±0.04)和(0.58±0.06) (P＜0.05). 结论 VD大鼠海马及前额叶皮质5 HT、NE含量减少.文拉法辛能提高VD大鼠海马及前额叶皮质5-HT、NE含量,并进一步提高脑组织BDNF含量,改善大鼠学习记忆功能.
目的 研究文拉法辛對血管性癡呆(VD)模型大鼠學習記憶功能的影響及機製. 方法 將Wistar大鼠隨機分為假手術組、模型組和治療組,各30隻.模型組和治療組採用改良Pulsinellis四血管阻斷法建立血管性癡呆大鼠模型.造模後第3天開始假手術組及模型組予蒸餾水灌胃,治療組予文拉法辛灌胃,劑量15 mg/kg,共28 d.採用單程逃避實驗測試各組大鼠的主動學習記憶能力,併觀察各組大鼠海馬及前額葉皮質5羥色胺(5-HT)、去甲腎上腺素(NE)含量及認腦源性神經營養因子(BDNF)含量變化. 結果 造模後30 d與假手術組大鼠單程逃避實驗完成逃避的百分比數(92.3±5.6)相比,模型組完成逃避的百分比數(50.3±6.2)明顯減少(P＜0.01),模型組大鼠海馬及前額葉皮質5-HT、NE含量明顯下降,分彆為(226±34) pg/g和(340±40) pg/g,(601±66)pg/g和(657±43) pg/g(P＜0.01),BDNF含量升高,海馬和前額葉皮質中吸光度分彆為(0.50±0.04)和(0.49±0.04) (P＜0.05).與模型組相比,治療組大鼠單程逃避實驗完成逃避的百分比數明顯增加至(62.2±4.6)(P＜0.05),大鼠海馬及前額葉皮質5-HT、NE含量升高,分彆為(264±45)pg/g和(379±42)pg/g,(798±51)pg/g和(798±51) pg/g(P＜0.05),BDNF含量進一步升高,海馬和前額葉皮質中吸光度分彆為(0.58±0.04)和(0.58±0.06) (P＜0.05). 結論 VD大鼠海馬及前額葉皮質5 HT、NE含量減少.文拉法辛能提高VD大鼠海馬及前額葉皮質5-HT、NE含量,併進一步提高腦組織BDNF含量,改善大鼠學習記憶功能.
목적 연구문랍법신대혈관성치태(VD)모형대서학습기억공능적영향급궤제. 방법 장Wistar대서수궤분위가수술조、모형조화치료조,각30지.모형조화치료조채용개량Pulsinellis사혈관조단법건립혈관성치태대서모형.조모후제3천개시가수술조급모형조여증류수관위,치료조여문랍법신관위,제량15 mg/kg,공28 d.채용단정도피실험측시각조대서적주동학습기억능력,병관찰각조대서해마급전액협피질5간색알(5-HT)、거갑신상선소(NE)함량급인뇌원성신경영양인자(BDNF)함량변화. 결과 조모후30 d여가수술조대서단정도피실험완성도피적백분비수(92.3±5.6)상비,모형조완성도피적백분비수(50.3±6.2)명현감소(P＜0.01),모형조대서해마급전액협피질5-HT、NE함량명현하강,분별위(226±34) pg/g화(340±40) pg/g,(601±66)pg/g화(657±43) pg/g(P＜0.01),BDNF함량승고,해마화전액협피질중흡광도분별위(0.50±0.04)화(0.49±0.04) (P＜0.05).여모형조상비,치료조대서단정도피실험완성도피적백분비수명현증가지(62.2±4.6)(P＜0.05),대서해마급전액협피질5-HT、NE함량승고,분별위(264±45)pg/g화(379±42)pg/g,(798±51)pg/g화(798±51) pg/g(P＜0.05),BDNF함량진일보승고,해마화전액협피질중흡광도분별위(0.58±0.04)화(0.58±0.06) (P＜0.05). 결론 VD대서해마급전액협피질5 HT、NE함량감소.문랍법신능제고VD대서해마급전액협피질5-HT、NE함량,병진일보제고뇌조직BDNF함량,개선대서학습기억공능.
Objective To investigate the effect of venlafaxine on learning and memory ability in rats with vascular dementia(VD).Methods Totally 90 Wistar rats were divided into sham-operation group,model group and treatment group (n=30 for each).The VD rat model was established by modified pulsinellis 4-vessel occlusion (4 VO).Rats in the sham-operation group and model group were administered with gastric perfusion of distilled water.Rats in the treatment group were administrated with gastric perfusion of Venlafaxine at 15 mg/kg per day for 4 weeks from the 2nd day after the modeling.The one-way avoidance test was performed to study the learning-memory ability of each group.The contents of norepinephrine,5-hydroxytryptamine and BDNF in the hippocampus and cortex of rats were observed.Results Model group demonstrated a decrease in the percentage of one-way avoidance test (50.3±6.2 vs.92.3±5.6,P＜0.01) as compared with sham-operation group,and this value was increased in treatment group (62 2±4.6).Compared with the sham-operation group,the contents of NE and 5-HT in the hippocampus [(226±34) pg/g and (340±40) pg/g],and cortex [(601±66) pg/g and (657±43) pg/g] in rat brains of model group were decreased (P＜0.01),while increased in treatment group [(264±45) pg/g and (379±42)pg/g,(665±68) pg/g and (798±51)pg/g,P＜0.05].The OD value of BDNF in the hippocampus (0.495±0.041) and cortex (0.488±0.042) were increased (P＜0.05) in model group,and in treatment group,BDNF levels were more higher (0.579±0.044 and 0.578±0.06/4,P＜0.05).Conclusions The content of brain monoamine neurotransmitters are decreased in rats with VD,while venlafaxine can improve the learning memory ability in model rats through increasing the levels of 5-HT,NE and BDNF in hippocampus and cortex.
