CAJ | 학술논문

目的了解广西巴马地区与城市(南宁市)80岁以上健康老年人的血清蛋白质谱状况,为探索巴马地区长寿的奥秘提供新的线索. 方法运用表面增强激光解析电离飞行时间质谱(SELDI-TOF-MS)技术,采用弱阳离子交换(CM10)、金属离子螯合(IMAC30-Cu)、强阴离子交换(Q10)、疏水(H50)4种芯片分别检测巴马地区与城市80及岁以上健康老年人的血清蛋白质谱.结果巴马地区与城市健康老年人血清中共检测到蛋白质(或多肽)峰:CM10 71个,IMAC30-Cu 46个,Q10 81个,H50 52个；巴马地区与城市健康老年人的血清蛋白质谱峰比较(P＜0.05且各蛋白质峰值的均数＞标准差),CM10芯片共检出8个差异蛋白质谱峰,其中2个表达上调、6个表达下调；IMAC-Cu芯片共检出7个差异蛋白质谱峰,其中1个表达上调,6个表达下调；Q10芯片共检出5个差异蛋白质谱峰,均为表达下调；H50芯片共检出6个差异蛋白质谱峰,其中3个表达上调,3个表达下调. 结论巴马地区与城市健康老年人的血清蛋白质谱存在一定的差异,且表达下调者居多.
목적 료해엄서파마지구여성시(남저시)80세이상건강노년인적혈청단백질보상황,위탐색파마지구장수적오비제공신적선색. 방법 운용표면증강격광해석전리비행시간질보(SELDI-TOF-MS)기술,채용약양리자교환(CM10)、금속리자오합(IMAC30-Cu)、강음리자교환(Q10)、소수(H50)4충심편분별검측파마지구여성시80급세이상건강노년인적혈청단백질보.결과 파마지구여성시건강노년인혈청중공검측도단백질(혹다태)봉:CM10 71개,IMAC30-Cu 46개,Q10 81개,H50 52개；파마지구여성시건강노년인적혈청단백질보봉비교(P＜0.05차각단백질봉치적균수＞표준차),CM10심편공검출8개차이단백질보봉,기중2개표체상조、6개표체하조；IMAC-Cu심편공검출7개차이단백질보봉,기중1개표체상조,6개표체하조；Q10심편공검출5개차이단백질보봉,균위표체하조；H50심편공검출6개차이단백질보봉,기중3개표체상조,3개표체하조. 결론 파마지구여성시건강노년인적혈청단백질보존재일정적차이,차표체하조자거다.
Objective To compare the serum protein profiles between people with successful aged 80 years and over in Bama versus Nanning,to identify the possible causes of the long life expectancy in Bama.Methods We evaluated the serum protein profiles using surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) with 4 different chromatographic surfaces,including the weak cation exchange (CM10),immobilized metal affinity capture (IMAC) 30-Cu,strong anion exchange (Q10) and hydrophobic surface (H50) protein chip arrays in people with successful aged 80 years and over living in Bama versus in Nanning city.SELDI-TOF-MS had a molecular mass detection range of 2000-50,000 D,with a first signal-to noise (S/N) ratio of 5,frequency of peak cluster of over 15 ％,and second S/N ratio of 2 and peak fluctuation of 0.3％ (m/z).Results The total numbers of serum protein (peptide) peaks for people with successful aging in Bama and Nanning were 71 on CM10,46 on IMAC30-Cu,81 on Q10,and 52 on H50.Among subjects from Bama as compared with Nanning (P＜0.05; mean ＞SD for each protein peak),there were 8 differently expressed protein peaks on CM10,of which 2 were upregulated and 6 downregulated; 7 on IMAC-Cu,of which 2 were upregulated and 6 downregulated; 5 on Q10,all of which were upregulated; and 6 on H50,of which 3 were upregulated and 3 downregulated.Conclusions There are marked differences in the serum protein profiles between people with successful aging living in Bama versus in Nanning.Most of the proteins are downregulated among people aged over 80 years in Bama.