中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2013年
1期
41-44
,共4页
郭锋%董利民%刘冬明%况九龙
郭鋒%董利民%劉鼕明%況九龍
곽봉%동이민%류동명%황구룡
基质金属蛋白酶类%多态性,限制性片段长度%肺疾病,慢性阻塞性
基質金屬蛋白酶類%多態性,限製性片段長度%肺疾病,慢性阻塞性
기질금속단백매류%다태성,한제성편단장도%폐질병,만성조새성
Matrix metalloproteinases%Polymorphism,restriction fragment length%Pulmonary disease,chronic obstructive
目的 探讨去整合素基质金属蛋白酶19(ADAM19)基因多态性与慢性阻塞性肺疾病(COPD)易感性的关系. 方法 收集116例COPD患者(COPD组)和82例健康者(对照组)外周血,提取全血细胞DNA,采用聚合酶链式反应-限制性片段长度多态性法检测ADAM19(rs1422795)位点基因多态性,对等位基因及基因型分布进行统计学分析. 结果 COPD组中ADAM19的AA、AG、GG基因型频率分别为73.3％、22.4％、4.3％,与对照组68.3％、28.0％、3.7％相比差异无统计学意义(x2=0.887,P=0.634)；COPD组中A、G等位基因频率分别为84.5％、15.5％,与对照组82.3％、17.7％相比差异无统计学意义(x2=0.582,P=0.446).ADAM19基因型分布在对照组和不同严重程度COPD患者中差异无统计学意义(x2＝3.325,P＝0.787). 结论 ADAM19(rs1422795)位点基因多态性与COPD无明显相关,ADAM19(rs1422795)可能不是COPD的易感基因.
目的 探討去整閤素基質金屬蛋白酶19(ADAM19)基因多態性與慢性阻塞性肺疾病(COPD)易感性的關繫. 方法 收集116例COPD患者(COPD組)和82例健康者(對照組)外週血,提取全血細胞DNA,採用聚閤酶鏈式反應-限製性片段長度多態性法檢測ADAM19(rs1422795)位點基因多態性,對等位基因及基因型分佈進行統計學分析. 結果 COPD組中ADAM19的AA、AG、GG基因型頻率分彆為73.3％、22.4％、4.3％,與對照組68.3％、28.0％、3.7％相比差異無統計學意義(x2=0.887,P=0.634)；COPD組中A、G等位基因頻率分彆為84.5％、15.5％,與對照組82.3％、17.7％相比差異無統計學意義(x2=0.582,P=0.446).ADAM19基因型分佈在對照組和不同嚴重程度COPD患者中差異無統計學意義(x2＝3.325,P＝0.787). 結論 ADAM19(rs1422795)位點基因多態性與COPD無明顯相關,ADAM19(rs1422795)可能不是COPD的易感基因.
목적 탐토거정합소기질금속단백매19(ADAM19)기인다태성여만성조새성폐질병(COPD)역감성적관계. 방법 수집116례COPD환자(COPD조)화82례건강자(대조조)외주혈,제취전혈세포DNA,채용취합매련식반응-한제성편단장도다태성법검측ADAM19(rs1422795)위점기인다태성,대등위기인급기인형분포진행통계학분석. 결과 COPD조중ADAM19적AA、AG、GG기인형빈솔분별위73.3％、22.4％、4.3％,여대조조68.3％、28.0％、3.7％상비차이무통계학의의(x2=0.887,P=0.634)；COPD조중A、G등위기인빈솔분별위84.5％、15.5％,여대조조82.3％、17.7％상비차이무통계학의의(x2=0.582,P=0.446).ADAM19기인형분포재대조조화불동엄중정도COPD환자중차이무통계학의의(x2＝3.325,P＝0.787). 결론 ADAM19(rs1422795)위점기인다태성여COPD무명현상관,ADAM19(rs1422795)가능불시COPD적역감기인.
Objective To explore the association between genetic polymorphism of a disintegrin and metalloprotease 19 (ADAM19) and chronic obstructive pulmonary disease (COPD).Methods Totally 116 patients with COPD (the COPD group) and 82 healthy volunteers (the control group) were enrolled in this study.Fasting peripheral blood was taken and whole blood corpuscle genomic DNA was extracted.The genetic polymorphism of ADAM19 (rs1422795) was determined by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).Results The difference in genotypes frequencies (GG,AG,AA) distribution of the ADAM19 between patients (73.3％,22.4％,and 4.3％) and controls (68.3％,28.0％,and 3.7％) showed no statistically significance (x2 =0.887,P=0.634).There were also no significant differences in the distribution of the different allele gene frequencies(A,G) between patients (84.5％,15.5％) and controls(82.3％,17.7％)(x2=0.582,P=0.446).No differences were found in the distribution of the genotypes between patients of different COPD severity classified according to FEV1 (％ pred),and healthy controls(x2=3.325,P=0.787).Conclusions ADAM19 (rs1422795) genetic polymorphism is not related to the development of COPD,which may not be the COPD-related gene.
