中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2014年
2期
197-200
,共4页
刘红梅%王翎%张光波%陈思文%潘旭东%毛燕青
劉紅梅%王翎%張光波%陳思文%潘旭東%毛燕青
류홍매%왕령%장광파%진사문%반욱동%모연청
抑制因子,免疫%抗原,CD%免疫耐受
抑製因子,免疫%抗原,CD%免疫耐受
억제인자,면역%항원,CD%면역내수
Suppressor factors,immunolagic%Antigens,CD%Immune tolerance
目的 探讨髓源性抑制细胞(MDSCs)负性共刺激分子(B7-H1)的表达及在增龄相关免疫抑制功能中的作用. 方法 健康C57BL/6老年(12月龄)及青年(4～8周龄)小鼠各18只.通过流式细胞分析法测定各组小鼠MDSCs上负性协同刺激因子的表达水平,并分别通过免疫磁珠分选法及小鼠淋巴结研磨方法,选得MDSCs及T细胞.T细胞增殖干预实验分5组进行:T细胞羟基荧光素二醋酸盐琥珀酰亚胺脂(CFSE)未标记组；T细胞CFSE标记组；T细胞标记CFSE+老年MDSCs组；T细胞标记CFSE+青年MDSCs组以及T细胞标记CFSE+老年MDSCs+ B7-H1阻断抗体组,观察B7-H1在MDSCs抑制T细胞增殖过程中的作用. 结果 与青年组比较,老年组MDSCs B7-H1的表达明显增高(t=3.27,P＜0.05).老年组MDSCs能明显抑制T细胞的增殖(t=5.42,P＜0.05),而青年组MDSCs对T细胞的干预作用不明显(t=0.64,P＞0.05),老年MDSCs加B7-H1阻断抗体组T细胞增殖被明显逆转,差异有统计学意义(t=8.28,P＜0.05). 结论 表达于MDSCs表面的B7-H1分子可能是增龄相关MDSCs发挥免疫抑制功能的重要调节因子.
目的 探討髓源性抑製細胞(MDSCs)負性共刺激分子(B7-H1)的錶達及在增齡相關免疫抑製功能中的作用. 方法 健康C57BL/6老年(12月齡)及青年(4～8週齡)小鼠各18隻.通過流式細胞分析法測定各組小鼠MDSCs上負性協同刺激因子的錶達水平,併分彆通過免疫磁珠分選法及小鼠淋巴結研磨方法,選得MDSCs及T細胞.T細胞增殖榦預實驗分5組進行:T細胞羥基熒光素二醋痠鹽琥珀酰亞胺脂(CFSE)未標記組；T細胞CFSE標記組；T細胞標記CFSE+老年MDSCs組；T細胞標記CFSE+青年MDSCs組以及T細胞標記CFSE+老年MDSCs+ B7-H1阻斷抗體組,觀察B7-H1在MDSCs抑製T細胞增殖過程中的作用. 結果 與青年組比較,老年組MDSCs B7-H1的錶達明顯增高(t=3.27,P＜0.05).老年組MDSCs能明顯抑製T細胞的增殖(t=5.42,P＜0.05),而青年組MDSCs對T細胞的榦預作用不明顯(t=0.64,P＞0.05),老年MDSCs加B7-H1阻斷抗體組T細胞增殖被明顯逆轉,差異有統計學意義(t=8.28,P＜0.05). 結論 錶達于MDSCs錶麵的B7-H1分子可能是增齡相關MDSCs髮揮免疫抑製功能的重要調節因子.
목적 탐토수원성억제세포(MDSCs)부성공자격분자(B7-H1)적표체급재증령상관면역억제공능중적작용. 방법 건강C57BL/6노년(12월령)급청년(4～8주령)소서각18지.통과류식세포분석법측정각조소서MDSCs상부성협동자격인자적표체수평,병분별통과면역자주분선법급소서림파결연마방법,선득MDSCs급T세포.T세포증식간예실험분5조진행:T세포간기형광소이작산염호박선아알지(CFSE)미표기조；T세포CFSE표기조；T세포표기CFSE+노년MDSCs조；T세포표기CFSE+청년MDSCs조이급T세포표기CFSE+노년MDSCs+ B7-H1조단항체조,관찰B7-H1재MDSCs억제T세포증식과정중적작용. 결과 여청년조비교,노년조MDSCs B7-H1적표체명현증고(t=3.27,P＜0.05).노년조MDSCs능명현억제T세포적증식(t=5.42,P＜0.05),이청년조MDSCs대T세포적간예작용불명현(t=0.64,P＞0.05),노년MDSCs가B7-H1조단항체조T세포증식피명현역전,차이유통계학의의(t=8.28,P＜0.05). 결론 표체우MDSCs표면적B7-H1분자가능시증령상관MDSCs발휘면역억제공능적중요조절인자.
Objective To explore the expression of negative costimulatory molecule B7-H1 of myeloid derived suppressor cells(MDSCs) and its roles in age-related immunosuppressive functions.Methods 36 C57BL/6 mice were divided into 2 groups:the elderly group(12-month-old,n=18) and the young group(4-8-week-old,n=18).The expressive level of negative costimulatory molecules of MDSCs in the two groups was measured by flow cytometry(FCM).Besides,MDSCs were sorted by using MDSC isolation kit and T cells were obtained by grinding lymph nodes.To explore the influence of B7-H1 on the MDSCs' immunosuppressive functions,T cell proliferation and intervention assay was conducted using the five following groups:T cells group,T cells + CFSE group [T cells with carboxyfluorescein diacetate succinimidyl ester(CFSE)],T cells +-CFSE cocultured with MDSCs from the young group,T cells + CFSE cocultured with MDSCs from the elderly group,and T cells + CFSE cocultured with MDSCs from elderly group and anti-B7-H1 blocking antibody.The effect of B7-H1 on T cell proliferation in MDSCs were observed.Results The expression level of B7-H1 of the MDSCs was significantly increased in the elderly group as compared with the young group(t=3.27,P＜0.05).T cell proliferation assay revealed that T cells were remarkably suppressed by MDSCs in elderly group as compared to the youth group(t=5.42,P＜0.05).The suppression of T cells by MDSCs was dramatically reversed by anti-B7-H1 blocking antibody in elderly group(t=8.28,P＜0.05).Conclusions B7-H1,a molecule expressed on the surface of MDSCs,plays a key role in the age-related immunosuppressive function of MDSCs.
