中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2014年
3期
257-260
,共4页
前列腺癌%受体,糖皮质激素%热休克蛋白%白细胞介素6%雄激素
前列腺癌%受體,糖皮質激素%熱休剋蛋白%白細胞介素6%雄激素
전렬선암%수체,당피질격소%열휴극단백%백세포개소6%웅격소
Prostate cancer%Receptors,glucocorticoid%Heat shock protein%Interleukin-6%Androgens
目的 探讨糖皮质激素受体(GR)信号通路及下游炎症因子白细胞介素(IL-6)等在前列腺癌雄激素非依赖生长中的作用. 方法 体外培养人雄激素依赖性前列腺癌(ADPC)细胞株LNCaP和雄激素非依赖性前列腺癌(AIPC)细胞株DU145,免疫细胞化学检测雄激素受体(AR)、GR、热休克蛋白(HSP90)和IL-6表达的差异,观察GR阻断剂RU486对LNCaP和DU145生长的影响,GR阻断后DU145细胞HSP90、IL-6 mRNA及蛋白表达的变化. 结果 LNCaP细胞AR+,GR,而DU145细胞GR+,AR,DU145细胞HSP90、IL-6表达明显强于LNCaP细胞(P＜0.01);RU486对LNCaP细胞生长的影响很小,而对DU145细胞呈现出明显的浓度和时间依赖性抑制作用;RU486作用后DU145细胞中HSP90、IL-6 mRNA及蛋白的表达明显降低. 结论 GR信号通路是DU145细胞的主要成活途径,GR信号通路的异常活化并促进炎症因子HSP90、IL-6等的高表达是前列腺癌雄激素非依赖进展的重要原因.
目的 探討糖皮質激素受體(GR)信號通路及下遊炎癥因子白細胞介素(IL-6)等在前列腺癌雄激素非依賴生長中的作用. 方法 體外培養人雄激素依賴性前列腺癌(ADPC)細胞株LNCaP和雄激素非依賴性前列腺癌(AIPC)細胞株DU145,免疫細胞化學檢測雄激素受體(AR)、GR、熱休剋蛋白(HSP90)和IL-6錶達的差異,觀察GR阻斷劑RU486對LNCaP和DU145生長的影響,GR阻斷後DU145細胞HSP90、IL-6 mRNA及蛋白錶達的變化. 結果 LNCaP細胞AR+,GR,而DU145細胞GR+,AR,DU145細胞HSP90、IL-6錶達明顯彊于LNCaP細胞(P＜0.01);RU486對LNCaP細胞生長的影響很小,而對DU145細胞呈現齣明顯的濃度和時間依賴性抑製作用;RU486作用後DU145細胞中HSP90、IL-6 mRNA及蛋白的錶達明顯降低. 結論 GR信號通路是DU145細胞的主要成活途徑,GR信號通路的異常活化併促進炎癥因子HSP90、IL-6等的高錶達是前列腺癌雄激素非依賴進展的重要原因.
목적 탐토당피질격소수체(GR)신호통로급하유염증인자백세포개소(IL-6)등재전렬선암웅격소비의뢰생장중적작용. 방법 체외배양인웅격소의뢰성전렬선암(ADPC)세포주LNCaP화웅격소비의뢰성전렬선암(AIPC)세포주DU145,면역세포화학검측웅격소수체(AR)、GR、열휴극단백(HSP90)화IL-6표체적차이,관찰GR조단제RU486대LNCaP화DU145생장적영향,GR조단후DU145세포HSP90、IL-6 mRNA급단백표체적변화. 결과 LNCaP세포AR+,GR,이DU145세포GR+,AR,DU145세포HSP90、IL-6표체명현강우LNCaP세포(P＜0.01);RU486대LNCaP세포생장적영향흔소,이대DU145세포정현출명현적농도화시간의뢰성억제작용;RU486작용후DU145세포중HSP90、IL-6 mRNA급단백적표체명현강저. 결론 GR신호통로시DU145세포적주요성활도경,GR신호통로적이상활화병촉진염증인자HSP90、IL-6등적고표체시전렬선암웅격소비의뢰진전적중요원인.
Objective To study the effects of glucocorticoid receptor (GR) signal pathway and downstream cytokineson androgen-independent growth of prostate cancer (PC) cells.Methods The human androgen-dependent PC (ADPC) cell line LNCaP and androgen-independent PC (AIPC) cell line DU145 were cultured in vitro.Immunocytochemistry was used to examine the expressions of the androgen receptor (AR),GR,HSPg0 and interleukin-6 (IL-6).The GR antagonist RU486 was used to treat cultured cells,and the effects of RU486 on the proliferation of both cell lines were analyzed by MTT assay.Expressions of HSP90 and IL-6 mRNA and protein were assessed by RT-PCR and Western blotting respectively.Results LNCaP cells were AR-positive and GR-negative,whereas DU145 cells were GR-positive and AR-negative.The expressions of HSP90 and IL-6 were significantly stronger in DU145 cells than in LNCaP cells (P＜0.01).RU486 had no obviously effects on the growth of LNCaP cells,but exerted a significant time-and dose-dependent growth inhibition on DU145 cells.RU486 treatment in DU145 cells also resulted in a dose-dependent decrease in the expressions of HSP90 and IL-6 mRNA and protein.Conclusions GR signal pathway may be the main survival pathway for DU145 cells.Abnormal hyperactivation of GR signal pathway and its promoting the expressions of HSP90 and IL-6 may contribute to the progression of ADPC to AIPC after androgen ablation.
