CAJ | 학술논문

目的观察电针对去卵巢大鼠模型肠黏膜维生素D受体(VDR) mRNA和蛋白表达的影响,探讨针灸治疗绝经后骨质疏松症可能机制. 方法 60只3月龄SD健康雌性大鼠,随机分为手术组和假手术组,3个月造模成功后将手术组随机分为:模型组、假电针组、电针组、药物组.干预治疗12周后,测定腰椎及股骨骨密度,然后处死大鼠,提取小肠黏膜,采用荧光实时定量PCR和Western blot检测VDR mRNA和蛋白表达. 结果成功复制绝经后骨质疏松症模型,经电针组和药物组干预治疗12周后,其大鼠骨密度与模型组相比均有明显提高,荧光实时定量PCR和Westernblot检测结果显示电针组和药物组大鼠小肠黏膜VDR mRNA和蛋白表达上调,但电针组上调VDRmRNA的水平不及药物组. 结论针灸治疗绝经后骨质疏松症机制之一可能与上调小肠黏膜VDR mRNA和蛋白表达量,增强了维生素D的生物活性有关.
목적 관찰전침대거란소대서모형장점막유생소D수체(VDR) mRNA화단백표체적영향,탐토침구치료절경후골질소송증가능궤제. 방법 60지3월령SD건강자성대서,수궤분위수술조화가수술조,3개월조모성공후장수술조수궤분위:모형조、가전침조、전침조、약물조.간예치료12주후,측정요추급고골골밀도,연후처사대서,제취소장점막,채용형광실시정량PCR화Western blot검측VDR mRNA화단백표체. 결과 성공복제절경후골질소송증모형,경전침조화약물조간예치료12주후,기대서골밀도여모형조상비균유명현제고,형광실시정량PCR화Westernblot검측결과현시전침조화약물조대서소장점막VDR mRNA화단백표체상조,단전침조상조VDRmRNA적수평불급약물조. 결론 침구치료절경후골질소송증궤제지일가능여상조소장점막VDR mRNA화단백표체량,증강료유생소D적생물활성유관.
Objective To observe the effect of electroacupuncture (EA) on intestinal vitamin D receptor (VDR) mRNA and protein expression in ovariectomized rat model,and to explore the possible mechanism of acupuncture treatment for postmenopausal osteoporosis.Methods Sixty 3-month-old female SD rats were randomly divided into operation group and sham group.3 months after osteoporosis model was successfully produced,rats in operation group were randomly divided into model group,EA sham group,EA group and drug group.12 weeks after intervention,bone mineral density (BMD) of lumbar spine and femur were detected,then rats were all sacrificed to extract the small intestinal mucosa.VDR mRNA and protein expressions were determined by real-time quantitative PCR and Western blotting.Results The model of postmenopausal osteoporosis was successfully established.The bone mineral density was significantly improved in EA group and drug group as compared with the model group at 12 weeks after intervention.Western blotting and realtime quantitative PCR showed that small intestinal VDR mRNA and protein expressions were increased in EA group and drug group,but the increment of VDR mRNA expression was less in EA group than in drug group.Conclusions One of the mechanisms of acupuncture treatment for postmenopausal osteoporosis may be related with increasing the intestinal VDR mRNA and protein expression levels and enhancing the bioactivity of vitamin D.