CAJ | 학술논문

目的探讨轻度认知功能障碍(MCI)患者脑脊液和血清中血红素氧化酶-1(HO-1)的含量变化,及其与简易精神状态量表(MMSE)和长沙版蒙特利尔评估量表(MoCA)的相关性,为早期诊断MCI提供依据. 方法选择2011年6月至2013年6月我院住院患者,分为MCI组45例和健康对照组85例,收集两组患者的基本资料并进行MMSE和长沙版MoCA量表评估;采用双抗体夹心酶联免疫吸附法(ELISA)检测并比较两组脑脊液和血清中HO-1的含量. 结果 MCI组35例平均血清HO-1含量(612.52±111.48)ng/L,健康对照组67例(384.16±56.86)ng/L,差异有统计学意义(t=2.03,P=0.045);MCI组26例平均脑脊液HO-1含量(631.38±32.17)ng/L,健康对照组66例(480.75±17.98)ng/L,差异有统计学意义(t=4.09,P=0.000).而MCI组、健康对照组血清HO-1含量和脑脊液HO-1含量比较差异均无统计学意义(t=0.14、1.61,P=0.887、0.110).MCI组患者脑脊液和血清中HO-1含量均与MMSE评分呈正相关(r=0.398、0.363 P=0.044、0.032),与MoCA得分相关性无统计学意义(均P＞0.05);对照组脑脊液和血清中HO-1含量变化均与MMSE评分呈负相关(r=-0.572、-0.285,P=0.000、0.019),脑脊液中HO-1含量与MoCA得分呈负相关(r=-0.440,P=0.000),血清中HO-1含量与MoCA得分相关性无统计学意义(P＞0.05).两组血清和脑脊液中HO-1含量与年龄的相关性均无统计学意义(均P＞0.05). 结论 MCI患者脑脊液和血清HO-1含量均明显高于非MCI者,且HO-1含量变化与MMSE评分成正相关,HO-1升高可提示MCI及认知功能下降,有望成为早期诊断MCI的新的标志物.
목적 탐토경도인지공능장애(MCI)환자뇌척액화혈청중혈홍소양화매-1(HO-1)적함량변화,급기여간역정신상태량표(MMSE)화장사판몽특리이평고량표(MoCA)적상관성,위조기진단MCI제공의거. 방법 선택2011년6월지2013년6월아원주원환자,분위MCI조45례화건강대조조85례,수집량조환자적기본자료병진행MMSE화장사판MoCA량표평고;채용쌍항체협심매련면역흡부법(ELISA)검측병비교량조뇌척액화혈청중HO-1적함량. 결과 MCI조35례평균혈청HO-1함량(612.52±111.48)ng/L,건강대조조67례(384.16±56.86)ng/L,차이유통계학의의(t=2.03,P=0.045);MCI조26례평균뇌척액HO-1함량(631.38±32.17)ng/L,건강대조조66례(480.75±17.98)ng/L,차이유통계학의의(t=4.09,P=0.000).이MCI조、건강대조조혈청HO-1함량화뇌척액HO-1함량비교차이균무통계학의의(t=0.14、1.61,P=0.887、0.110).MCI조환자뇌척액화혈청중HO-1함량균여MMSE평분정정상관(r=0.398、0.363 P=0.044、0.032),여MoCA득분상관성무통계학의의(균P＞0.05);대조조뇌척액화혈청중HO-1함량변화균여MMSE평분정부상관(r=-0.572、-0.285,P=0.000、0.019),뇌척액중HO-1함량여MoCA득분정부상관(r=-0.440,P=0.000),혈청중HO-1함량여MoCA득분상관성무통계학의의(P＞0.05).량조혈청화뇌척액중HO-1함량여년령적상관성균무통계학의의(균P＞0.05). 결론 MCI환자뇌척액화혈청HO-1함량균명현고우비MCI자,차HO-1함량변화여MMSE평분성정상관,HO-1승고가제시MCI급인지공능하강,유망성위조기진단MCI적신적표지물.
Objective To explore the change in heme oxygenase-1 (HO-1) level in cerebrospinal fluid(CSF)and serum in patients with mild cognitive impairment(MCI),and the correlation between HO-1 and the rating scale,to provide a new marker for the diagnosis of MCI.Methods The HO-1 levels in CSF and serum in 45 MCI patients (MCI group) and 85 normal cases (control group) were analyzed with sensitive enzyme linked immunosorbent assays (ELISA).MMSE and MoCA scores were evaluated.Results The level of HO-1 was higher in MCI group than in control group both in CSF [(631.38±32.17)vs(480.75±17.98)ng/ L,P＜0.05],and in serum [(612.52±111.48)vs.(384.16±56.86)ng/ L,P＜0.05].The MCI and normal people HO-1 level had no significant difference between CSF group and serum group (P＞0.05).In MCI group,the levels of serum and CSF HO-1 had a positive correlation with MMSE scores (P＜0.05),but had no obvious correlation with MoCA scores (P＞0.05).In the normal group,the level of HO-1 was negatively related with MMSE scores in serum and CSF,and with MoCA scores in CSF (P＜0.05),but no obvious correlation in serum (P＞0.05).The levels of serum and CSF HO-1 had no obvious correlation with age in both groups (P＞0.05).Conclusions HO-1 concentration in both CSF and serum is significantly higher in MCI group than in normal group,and positively related with MMSE score.Thus the increase of HO-1concentration in both CSF and/or serum might be a new marker for the diagnosis of MCI.