中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2014年
7期
792-794
,共3页
神经元%端粒重复序列结合蛋白质2
神經元%耑粒重複序列結閤蛋白質2
신경원%단립중복서렬결합단백질2
Neurons%Telomeric repeat binding protein 2
目的 观察小鼠大脑皮层端粒重复序列结合因子2(telomeric repeat binding factor 2,TRF2)表达的增龄性改变,探讨TRF2基因转染对体外培养皮层神经元的保护作用及TRF2在神经元衰老中的作用. 方法 将C57BL/6J小鼠分为青年组(2月龄)和老年组(20月龄),分别采用蛋白质免疫印迹法(Western blot)和实时定量聚合酶链反应(Real-time PCR)检测小鼠大脑皮层TRF2表达;pcDNA-TRF2质粒转染孕18 d胎鼠皮层神经元细胞,喜树碱作用16h,四甲基偶氮唑蓝(MTT)比色法检测神经元细胞存活率. 结果 与2个月龄组小鼠比较,20个月龄组小鼠大脑皮层TRF2表达明显降低;喜树碱作用16h后,转染pcDNA-TRF2的神经元存活率为(75.4±2.6)％,明显高于与转染空载体质粒组(32.6±9.3)％(t=22.85,P＜0.05). 结论 TFR2表达降低参与神经元衰老,TRF2过表达可能是治疗神经退行性变的新靶点.
目的 觀察小鼠大腦皮層耑粒重複序列結閤因子2(telomeric repeat binding factor 2,TRF2)錶達的增齡性改變,探討TRF2基因轉染對體外培養皮層神經元的保護作用及TRF2在神經元衰老中的作用. 方法 將C57BL/6J小鼠分為青年組(2月齡)和老年組(20月齡),分彆採用蛋白質免疫印跡法(Western blot)和實時定量聚閤酶鏈反應(Real-time PCR)檢測小鼠大腦皮層TRF2錶達;pcDNA-TRF2質粒轉染孕18 d胎鼠皮層神經元細胞,喜樹堿作用16h,四甲基偶氮唑藍(MTT)比色法檢測神經元細胞存活率. 結果 與2箇月齡組小鼠比較,20箇月齡組小鼠大腦皮層TRF2錶達明顯降低;喜樹堿作用16h後,轉染pcDNA-TRF2的神經元存活率為(75.4±2.6)％,明顯高于與轉染空載體質粒組(32.6±9.3)％(t=22.85,P＜0.05). 結論 TFR2錶達降低參與神經元衰老,TRF2過錶達可能是治療神經退行性變的新靶點.
목적 관찰소서대뇌피층단립중복서렬결합인자2(telomeric repeat binding factor 2,TRF2)표체적증령성개변,탐토TRF2기인전염대체외배양피층신경원적보호작용급TRF2재신경원쇠로중적작용. 방법 장C57BL/6J소서분위청년조(2월령)화노년조(20월령),분별채용단백질면역인적법(Western blot)화실시정량취합매련반응(Real-time PCR)검측소서대뇌피층TRF2표체;pcDNA-TRF2질립전염잉18 d태서피층신경원세포,희수감작용16h,사갑기우담서람(MTT)비색법검측신경원세포존활솔. 결과 여2개월령조소서비교,20개월령조소서대뇌피층TRF2표체명현강저;희수감작용16h후,전염pcDNA-TRF2적신경원존활솔위(75.4±2.6)％,명현고우여전염공재체질립조(32.6±9.3)％(t=22.85,P＜0.05). 결론 TFR2표체강저삼여신경원쇠로,TRF2과표체가능시치료신경퇴행성변적신파점.
Objective To observe expression changes of the telomeric repeat binding factor 2 (TRF2) in mouse cortical neurons during aging and its biological significance.Methods TRF2 expression in cortical neurons of young (2 months) and old (20 months) C57BL/6J mice were tested by Western blot and real time PCR.pcDNA-TRF2 was transfected in embryonal cortical neurons.Neurons viability was determined by MTT after exposure to camptothecin for 16 h.Results TRF2 expression decreased significantly in cortical neurons in old mice than that in young mice.After exposure to camptothecin for 16 h,(75.4±2.6) ％ of pcDNA TRF2 transfected neurons were viable and the transfection rate was higher in pcDNA-TRF2 transfected neurons than in control transfected neurons [(32.6 ± 9.3) ％] (t =22.85,P ＜ 0.05).Conclusions TRF2 expression decreases significantly in aging mouse,downregulation of TRF2 may participate in neurons aging,and TRF2 overexpression may be a potential therapeutic target against neurodegeneration.
