中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2014年
8期
887-890
,共4页
陈敏敏%郑松柏%肖立%俞彰%Dewan Sheilesh Kumar
陳敏敏%鄭鬆柏%肖立%俞彰%Dewan Sheilesh Kumar
진민민%정송백%초립%유창%Dewan Sheilesh Kumar
幽门窦%胃黏膜
幽門竇%胃黏膜
유문두%위점막
Pyloric antrum%Gastric mucosa
目的 探讨人体胃窦幽门腺组织学和超微结构的增龄变化特点. 方法 选择拥有相对正常胃组织的60例患者为研究对象,包括中青年组(20～59岁)20例,老年组(≥60岁)40例.老年组包括60～69岁组15例,70～79岁组13例,80～89岁组12例.在光学显微镜下测定各组胃窦固有膜厚度、幽门腺实质比,对比观察各组胃窦固有膜、幽门腺及腺细胞形态;在电子显微镜下对比观察各组幽门腺细胞的超微结构. 结果 胃窦固有膜厚度20～59岁组为(649.8±127.9)μm,60～69岁组为(634.6±121.4)μm,70～79岁组为(615.7±74.6)μm,随增龄呈降低趋势;但80～89岁组为(645.5±99.9)μm,与20～59岁组相当(t=0.080,P=0.937).幽门腺实质比从20～59岁组、60～69岁组、70～79岁组到80～89岁组呈明显随增龄降低趋势,依次为(37.6±6.5)％、(34.7±5.0)％、(31.5±6.4)％和(28.3±4.7)％(F=5.010,P=0.005);老年组幽门腺实质比例明显低于中青年组(t=3.181,P=0.003).光学显微镜下显示,老年各亚组(尤其是80～89岁)与中青年组比较,胃窦固有膜内幽门腺腺体排列较稀疏,间质结缔组织增生明显,幽门腺细胞核增大、深染、细胞边界模糊.电子显微镜下显示,各组幽门腺细胞及与分泌黏液相关的细胞器形态分布无明显差异;定量测定黏原颗粒面积分数显示呈明显随增龄降低趋势(F=19.831,P=0.000),老年组(25.68±4.62)％明显低于中青年组(34.66±4.34)％(t=-6.456,P=0.000). 结论 随增龄,人体胃窦固有膜厚度有降低趋势,幽门腺腺体密度逐渐降低,腺体间结缔组织增生逐渐增加,幽门腺细胞内与分泌黏液有关的黏原颗粒密度逐渐降低,提示老年人胃窦黏膜及其功能腺体在形态学上存在退化现象.
目的 探討人體胃竇幽門腺組織學和超微結構的增齡變化特點. 方法 選擇擁有相對正常胃組織的60例患者為研究對象,包括中青年組(20～59歲)20例,老年組(≥60歲)40例.老年組包括60～69歲組15例,70～79歲組13例,80～89歲組12例.在光學顯微鏡下測定各組胃竇固有膜厚度、幽門腺實質比,對比觀察各組胃竇固有膜、幽門腺及腺細胞形態;在電子顯微鏡下對比觀察各組幽門腺細胞的超微結構. 結果 胃竇固有膜厚度20～59歲組為(649.8±127.9)μm,60～69歲組為(634.6±121.4)μm,70～79歲組為(615.7±74.6)μm,隨增齡呈降低趨勢;但80～89歲組為(645.5±99.9)μm,與20～59歲組相噹(t=0.080,P=0.937).幽門腺實質比從20～59歲組、60～69歲組、70～79歲組到80～89歲組呈明顯隨增齡降低趨勢,依次為(37.6±6.5)％、(34.7±5.0)％、(31.5±6.4)％和(28.3±4.7)％(F=5.010,P=0.005);老年組幽門腺實質比例明顯低于中青年組(t=3.181,P=0.003).光學顯微鏡下顯示,老年各亞組(尤其是80～89歲)與中青年組比較,胃竇固有膜內幽門腺腺體排列較稀疏,間質結締組織增生明顯,幽門腺細胞覈增大、深染、細胞邊界模糊.電子顯微鏡下顯示,各組幽門腺細胞及與分泌黏液相關的細胞器形態分佈無明顯差異;定量測定黏原顆粒麵積分數顯示呈明顯隨增齡降低趨勢(F=19.831,P=0.000),老年組(25.68±4.62)％明顯低于中青年組(34.66±4.34)％(t=-6.456,P=0.000). 結論 隨增齡,人體胃竇固有膜厚度有降低趨勢,幽門腺腺體密度逐漸降低,腺體間結締組織增生逐漸增加,幽門腺細胞內與分泌黏液有關的黏原顆粒密度逐漸降低,提示老年人胃竇黏膜及其功能腺體在形態學上存在退化現象.
목적 탐토인체위두유문선조직학화초미결구적증령변화특점. 방법 선택옹유상대정상위조직적60례환자위연구대상,포괄중청년조(20～59세)20례,노년조(≥60세)40례.노년조포괄60～69세조15례,70～79세조13례,80～89세조12례.재광학현미경하측정각조위두고유막후도、유문선실질비,대비관찰각조위두고유막、유문선급선세포형태;재전자현미경하대비관찰각조유문선세포적초미결구. 결과 위두고유막후도20～59세조위(649.8±127.9)μm,60～69세조위(634.6±121.4)μm,70～79세조위(615.7±74.6)μm,수증령정강저추세;단80～89세조위(645.5±99.9)μm,여20～59세조상당(t=0.080,P=0.937).유문선실질비종20～59세조、60～69세조、70～79세조도80～89세조정명현수증령강저추세,의차위(37.6±6.5)％、(34.7±5.0)％、(31.5±6.4)％화(28.3±4.7)％(F=5.010,P=0.005);노년조유문선실질비례명현저우중청년조(t=3.181,P=0.003).광학현미경하현시,노년각아조(우기시80～89세)여중청년조비교,위두고유막내유문선선체배렬교희소,간질결체조직증생명현,유문선세포핵증대、심염、세포변계모호.전자현미경하현시,각조유문선세포급여분비점액상관적세포기형태분포무명현차이;정량측정점원과립면적분수현시정명현수증령강저추세(F=19.831,P=0.000),노년조(25.68±4.62)％명현저우중청년조(34.66±4.34)％(t=-6.456,P=0.000). 결론 수증령,인체위두고유막후도유강저추세,유문선선체밀도축점강저,선체간결체조직증생축점증가,유문선세포내여분비점액유관적점원과립밀도축점강저,제시노년인위두점막급기공능선체재형태학상존재퇴화현상.
Objective To investigate the aging changes in the histology and ultrastructure of pyloric glands.Methods Sixty subjects with relatively normal gastric tissues were enrolled,with 20 subjects in the younger group (20-59 years) and 40 subjects in the elderly group (≥60 years).The latter group included 15 subjects aged from 60 to 69 years,13 subjects aged from 70 to 79 years and 12 subjects aged from 80 to 89 years.Under light microscopy,the histology and morphology of pyloric glands were observed to determine the thickness of the lamina propria and the Ap:Al ratio (Ap:area of the parenchyma,Al:the area of gastric mucosa lamina proprias),and to compare the morphology of the lamina propria,pyloric gland and gland cells in each group.The ultrastructure of pyloric gland cells was examined under electron microscope.Results From 20-59 age group to 60-69 age group to the 70-79 age group,the thickness of the lamina propria showed a decreasing trend from [(649.8± 127.9) μm to (634.6±121.4) μm and to (615.7 ± 74.6) μm,respectively,F=0.270,P=0.765],but the thickness of the lamina propria in 80-89 age group was increased to (645.5± 99.9) μm,comparable to that in the 20-59 age group.From 20-59 to 60-69 to 70-79 to 80-89 age group,the Ap to Al ratio (％) showed a significant decreasing trend from [(37.6 ± 6.5) ％ to (34.7 ± 5.0) ％ to (31.5 ± 6.4) ％ to (28.3 ± 4.7) ％,F=5.010,P=0.005] ; the average Ap to Al ratio in the elderly group was significantly lower(31.6±5.9) ％ than in the younger group(37.6±6.5) ％ (t=3.181,P=0.003).Interstitial hyperplasia of the lamina propria,sparse arrangement of pyloric glands,pyloric glands cells nucleienlarged and deeply stained and fuzzy cell boundaries were more clearly seen in the elderly group (especially 80-89 age group) as compared with the younger group.There was no significant difference in the ultrastructure of pyloric gland cells among each group.The average Am:Ac ratio (Am:the area of mucous granule,Ac:the area of cytoplasm of pyloric gland cell) showed a decreasing trend with age (F=19.831,P=0.000).The average Am:Ac ratio in the elderly group [(25.68±4.62) ％] was significantly lower than that in the younger group [(34.66 ±4.34) ％] (t=-6.456,P =0.000).Conclusions Along with aging,the thickness of the lamina propria has a decreasing trend,the density of pyloric glands is gradually decreased,while the connective tissue between the glands is increased.The density of mucous granules in pyloric cells associated with mucus secretion is also decreased.Hence,in the elderly,gastric antral mucosa and its functional glands have deteriorated performance and morphology.
