中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2014年
8期
911-915
,共5页
陈次滨%张永霞%张利洪%黎鹏飞
陳次濱%張永霞%張利洪%黎鵬飛
진차빈%장영하%장리홍%려붕비
丹皮甙%肌,平滑,血管%主动脉,腹%增殖细胞抗原
丹皮甙%肌,平滑,血管%主動脈,腹%增殖細胞抗原
단피대%기,평활,혈관%주동맥,복%증식세포항원
Paeonoside%Muscle,smooth,vascular%Aorta,abdominal%Proliferating cell nuclear antigen
目的 探讨丹皮酚对兔血管成形术后再狭窄的作用及其机制. 方法 选用清洁级雄性新西兰大白兔,采用腹主动脉球囊损伤和高脂饲料喂养方法建立腹主动脉粥样硬化模型,对模型兔腹主动脉狭窄段行2次球囊扩张后分为4组,分别为对照组、低剂量丹皮酚组、高剂量丹皮酚组、雷帕霉素组,分别给予生理盐水2 ml· kg-1·d-1、低剂量丹皮酚75 mg· kg-1·d-1、高剂量丹皮酚150mg·kg-1·d-1、雷帕霉素1.25 mg· kg-1·d-1灌胃,每天灌胃1次,连续6周给药.6周后取出腹主动脉,使用苏木精-伊红(HE)染色和Masson染色观察狭窄动脉的大体形态学及内膜增生厚度.免疫组化检测增殖细胞抗原(PCNA)表达水平,免疫印迹法(Western-blot)检测P27kip1蛋白表达水平.结果 使用丹皮酚、雷帕霉素后内膜增生程度降低.对照组、低剂量丹皮酚组、高剂量丹皮酚组和雷帕霉素组新生内膜面积分别为0.16±0.03、0.09±0.01、0.05±0.01、0.04±0.01;新生内膜面积/中膜面积分别为90.62±3.43、42.30±2.94、33.72±3.19、28.78±6.67;内膜面积增生率分别为(33.3±3.1)％、(23.2±2.2)％、(17.5±1.5)％、(16.2±1.7)％,差异有统计学意义(F值分别为47.98、257.25、74.60,均P＜0.05).对照组、低剂量丹皮酚组、高剂量丹皮酚组和雷帕霉素组PCNA的表达水平分别为0.20±0.04、0.06±0.02、0.05±0.02、0.08±0.02,差异均有统计学意义(F=37.22,P＜0.01);P27kip1表达水平分别为0.47±0.09、0.78±0.20、1.29±0.12、1.83±0.13,差异有统计学意义(F=107.69,P＜0.01). 结论 丹皮酚可能通过抑制PCNA表达,在细胞周期中上调P27kip1蛋白的表达,起到抗血管平滑肌细胞增殖的作用.
目的 探討丹皮酚對兔血管成形術後再狹窄的作用及其機製. 方法 選用清潔級雄性新西蘭大白兔,採用腹主動脈毬囊損傷和高脂飼料餵養方法建立腹主動脈粥樣硬化模型,對模型兔腹主動脈狹窄段行2次毬囊擴張後分為4組,分彆為對照組、低劑量丹皮酚組、高劑量丹皮酚組、雷帕黴素組,分彆給予生理鹽水2 ml· kg-1·d-1、低劑量丹皮酚75 mg· kg-1·d-1、高劑量丹皮酚150mg·kg-1·d-1、雷帕黴素1.25 mg· kg-1·d-1灌胃,每天灌胃1次,連續6週給藥.6週後取齣腹主動脈,使用囌木精-伊紅(HE)染色和Masson染色觀察狹窄動脈的大體形態學及內膜增生厚度.免疫組化檢測增殖細胞抗原(PCNA)錶達水平,免疫印跡法(Western-blot)檢測P27kip1蛋白錶達水平.結果 使用丹皮酚、雷帕黴素後內膜增生程度降低.對照組、低劑量丹皮酚組、高劑量丹皮酚組和雷帕黴素組新生內膜麵積分彆為0.16±0.03、0.09±0.01、0.05±0.01、0.04±0.01;新生內膜麵積/中膜麵積分彆為90.62±3.43、42.30±2.94、33.72±3.19、28.78±6.67;內膜麵積增生率分彆為(33.3±3.1)％、(23.2±2.2)％、(17.5±1.5)％、(16.2±1.7)％,差異有統計學意義(F值分彆為47.98、257.25、74.60,均P＜0.05).對照組、低劑量丹皮酚組、高劑量丹皮酚組和雷帕黴素組PCNA的錶達水平分彆為0.20±0.04、0.06±0.02、0.05±0.02、0.08±0.02,差異均有統計學意義(F=37.22,P＜0.01);P27kip1錶達水平分彆為0.47±0.09、0.78±0.20、1.29±0.12、1.83±0.13,差異有統計學意義(F=107.69,P＜0.01). 結論 丹皮酚可能通過抑製PCNA錶達,在細胞週期中上調P27kip1蛋白的錶達,起到抗血管平滑肌細胞增殖的作用.
목적 탐토단피분대토혈관성형술후재협착적작용급기궤제. 방법 선용청길급웅성신서란대백토,채용복주동맥구낭손상화고지사료위양방법건립복주동맥죽양경화모형,대모형토복주동맥협착단행2차구낭확장후분위4조,분별위대조조、저제량단피분조、고제량단피분조、뢰파매소조,분별급여생리염수2 ml· kg-1·d-1、저제량단피분75 mg· kg-1·d-1、고제량단피분150mg·kg-1·d-1、뢰파매소1.25 mg· kg-1·d-1관위,매천관위1차,련속6주급약.6주후취출복주동맥,사용소목정-이홍(HE)염색화Masson염색관찰협착동맥적대체형태학급내막증생후도.면역조화검측증식세포항원(PCNA)표체수평,면역인적법(Western-blot)검측P27kip1단백표체수평.결과 사용단피분、뢰파매소후내막증생정도강저.대조조、저제량단피분조、고제량단피분조화뢰파매소조신생내막면적분별위0.16±0.03、0.09±0.01、0.05±0.01、0.04±0.01;신생내막면적/중막면적분별위90.62±3.43、42.30±2.94、33.72±3.19、28.78±6.67;내막면적증생솔분별위(33.3±3.1)％、(23.2±2.2)％、(17.5±1.5)％、(16.2±1.7)％,차이유통계학의의(F치분별위47.98、257.25、74.60,균P＜0.05).대조조、저제량단피분조、고제량단피분조화뢰파매소조PCNA적표체수평분별위0.20±0.04、0.06±0.02、0.05±0.02、0.08±0.02,차이균유통계학의의(F=37.22,P＜0.01);P27kip1표체수평분별위0.47±0.09、0.78±0.20、1.29±0.12、1.83±0.13,차이유통계학의의(F=107.69,P＜0.01). 결론 단피분가능통과억제PCNA표체,재세포주기중상조P27kip1단백적표체,기도항혈관평활기세포증식적작용.
Objective To study the preventive effect of paeonol on restenosis after angioplasty in rabbit balloon injury model and its mechanism.Methods Male New Zealand white rabbits with clean grade were selected.Balloon injury rabbit abdominal aorta combined with high cholesterol diet were used to establish rabbit atherosclerosis model.We used balloon to dilate the narrow abdominal aorta.The animals were randomly divided into four groups (control group,low concentration paeonol group,high concentration paeonol group and rapamycin group).Animals in different group were orally given normal saline 2 ml · kg-1 · d-1,low concentration of paeonol 75 mg · kg-1 · d-1,high concentration of paeonol 150 mg · kg-1 · d-1,rapamycin administrated with 1.25 mg · kg-1 · d-1 for six weeks respectively.After 6 weeks,the animals were killed by air embolism,and immediately dissected to remove the abdominal aorta.We used hematoxylin and eosin(HE) staining and Masson staining to detect the thickness of the arterial intimal hyperplasia and the gross morphological changes of the narrow artery.Immunohistochemical staining was used to detect changes of proliferating cell nuclear antigen (PCNA) expression level.Western blotting was used to discover P27kip1 protein expression in the narrow artery.Results Paeonol and rapamycin reduced the degree of intimal hyperplasia.The neointima areas in the control group,low concentration paeonol group,high concentration paeonol group and rapamycin group were (0.16±0.03),(0.09±0.01),(0.05±0.01) and (0.04±0.01) respectively; the ratio of neointimal area to media area was (90.62 ±3.43),(42.30 ±2.94) (33.72±3.19) and (28.78±6.67) respectively; the intimal area proliferation rate was (33.3 ± 3.1) ％,(23.2 ± 2.2) ％,(17.5 ± 1.5) ％ and (16.2 ± 1.7) ％ respectively.There were significant differences in the neointima area,ratio of neointimal area to media area,and the intimal area proliferation rate among the four groups (F=47.98,257.25,74.60,all P＜0.05).The expression level of PCNA and P27kip1 protein were (0.20±0.04),(0.06±0.02),(0.05±0.02) and (0.08± 0.02),and (0.47±0.09),(0.78±0.20),(1.29±0.12) and (1.83±0.13) in the control group,low concentration paeonol group,high concentration paeonol group and rapamycin group respectively,and the differences were statistically significant between the four groups (F=37.22 and 107.69,both P＜0.01).Conclusions Paeonol plays a role in anti-vascular smooth muscle cell proliferation.This effect may be through inhibiting the expression of PCNA,and increasing the expression of P27kip1 in the cell cycle.
