中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2014年
9期
1014-1017
,共4页
蔡雨汐%徐静%万燕杰%张帆
蔡雨汐%徐靜%萬燕傑%張帆
채우석%서정%만연걸%장범
肝切除术%阿尔茨海默病%小鼠,转基因%认知%海马
肝切除術%阿爾茨海默病%小鼠,轉基因%認知%海馬
간절제술%아이자해묵병%소서,전기인%인지%해마
Hepatectomy%Alzheimer's disease%Mice,transgenic%Cognition%Hippocampus
目的 探讨APP/PS1转基因阿尔茨海默病(AD)模型小鼠肝部分切除术后空间学习记忆能力及海马Drebrin和PSD95蛋白表达的变化. 方法 3月龄APP/PS1转基因小鼠随机分为3组:3月龄对照组(6只)、假手术组(24只)、手术组(24只);3月龄野生型同窝对照组和6月龄对照组各6只.假手术组和手术组各分4个亚组,分别在麻醉或手术后1d、3d、7d、14d用水迷宫测试小鼠的学习记忆能力,同时用蛋白免疫印迹方法观察海马内Drebrin和PSD95蛋白的表达. 结果 与假手术组同时间点比较,手术后3d、7d、14 d组逃避潜伏期分别为(62.9±6.9)s、(66.3±9.5)s、(67.1±7.5)s,跨越平台次数分别为(2.1±0.7)次、(1.83±1.5)次、(2.5±1.9)次,水迷宫成绩明显下降(P＜0.05);手术后14 d组与6月龄对照组水迷宫成绩相接近;3月龄对照组、3月龄野生型同窝对照组和假手术组之间差异无统计学意义;Western blot显示与手术组同时间点相比,手术组术后1 d(0.30±0.01)、3 d(0.17±0.01)、7 d(0.05±0.002)、14 d(0.21±0.002) Drebrin表达下降(P＜0.05),手术后14 d组较手术后7d组表达增高(t=46.32,P＜0.01);与3月龄对照组相比,假手术后7d组(1.33±0.05) Drebrin表达增高(t=8.59,P＜0.01);与假手术组同时间点相比,手术后3d(0.44±0.02)、7 d(0.53±0.02)、14 d(0.21±0.02)PSD95表达下降(P＜0.05);与3月龄对照组相比,假手术后7d组(2.04±0.12)PSD95表达增高(t=32.79,P＜0.01);3月龄对照组与3月龄野生型同窝对照组相比,Drebrin和PSD95表达水平差异无统计学意义. 结论 肝叶部分切除术后3月龄APP/PS1转基因AD模型小鼠空间学习记忆能力下降与海马Drebrin和PSD95蛋白表达降低密切相关.
目的 探討APP/PS1轉基因阿爾茨海默病(AD)模型小鼠肝部分切除術後空間學習記憶能力及海馬Drebrin和PSD95蛋白錶達的變化. 方法 3月齡APP/PS1轉基因小鼠隨機分為3組:3月齡對照組(6隻)、假手術組(24隻)、手術組(24隻);3月齡野生型同窩對照組和6月齡對照組各6隻.假手術組和手術組各分4箇亞組,分彆在痳醉或手術後1d、3d、7d、14d用水迷宮測試小鼠的學習記憶能力,同時用蛋白免疫印跡方法觀察海馬內Drebrin和PSD95蛋白的錶達. 結果 與假手術組同時間點比較,手術後3d、7d、14 d組逃避潛伏期分彆為(62.9±6.9)s、(66.3±9.5)s、(67.1±7.5)s,跨越平檯次數分彆為(2.1±0.7)次、(1.83±1.5)次、(2.5±1.9)次,水迷宮成績明顯下降(P＜0.05);手術後14 d組與6月齡對照組水迷宮成績相接近;3月齡對照組、3月齡野生型同窩對照組和假手術組之間差異無統計學意義;Western blot顯示與手術組同時間點相比,手術組術後1 d(0.30±0.01)、3 d(0.17±0.01)、7 d(0.05±0.002)、14 d(0.21±0.002) Drebrin錶達下降(P＜0.05),手術後14 d組較手術後7d組錶達增高(t=46.32,P＜0.01);與3月齡對照組相比,假手術後7d組(1.33±0.05) Drebrin錶達增高(t=8.59,P＜0.01);與假手術組同時間點相比,手術後3d(0.44±0.02)、7 d(0.53±0.02)、14 d(0.21±0.02)PSD95錶達下降(P＜0.05);與3月齡對照組相比,假手術後7d組(2.04±0.12)PSD95錶達增高(t=32.79,P＜0.01);3月齡對照組與3月齡野生型同窩對照組相比,Drebrin和PSD95錶達水平差異無統計學意義. 結論 肝葉部分切除術後3月齡APP/PS1轉基因AD模型小鼠空間學習記憶能力下降與海馬Drebrin和PSD95蛋白錶達降低密切相關.
목적 탐토APP/PS1전기인아이자해묵병(AD)모형소서간부분절제술후공간학습기억능력급해마Drebrin화PSD95단백표체적변화. 방법 3월령APP/PS1전기인소서수궤분위3조:3월령대조조(6지)、가수술조(24지)、수술조(24지);3월령야생형동와대조조화6월령대조조각6지.가수술조화수술조각분4개아조,분별재마취혹수술후1d、3d、7d、14d용수미궁측시소서적학습기억능력,동시용단백면역인적방법관찰해마내Drebrin화PSD95단백적표체. 결과 여가수술조동시간점비교,수술후3d、7d、14 d조도피잠복기분별위(62.9±6.9)s、(66.3±9.5)s、(67.1±7.5)s,과월평태차수분별위(2.1±0.7)차、(1.83±1.5)차、(2.5±1.9)차,수미궁성적명현하강(P＜0.05);수술후14 d조여6월령대조조수미궁성적상접근;3월령대조조、3월령야생형동와대조조화가수술조지간차이무통계학의의;Western blot현시여수술조동시간점상비,수술조술후1 d(0.30±0.01)、3 d(0.17±0.01)、7 d(0.05±0.002)、14 d(0.21±0.002) Drebrin표체하강(P＜0.05),수술후14 d조교수술후7d조표체증고(t=46.32,P＜0.01);여3월령대조조상비,가수술후7d조(1.33±0.05) Drebrin표체증고(t=8.59,P＜0.01);여가수술조동시간점상비,수술후3d(0.44±0.02)、7 d(0.53±0.02)、14 d(0.21±0.02)PSD95표체하강(P＜0.05);여3월령대조조상비,가수술후7d조(2.04±0.12)PSD95표체증고(t=32.79,P＜0.01);3월령대조조여3월령야생형동와대조조상비,Drebrin화PSD95표체수평차이무통계학의의. 결론 간협부분절제술후3월령APP/PS1전기인AD모형소서공간학습기억능력하강여해마Drebrin화PSD95단백표체강저밀절상관.
Objective To study the influences of hepatolobectomy on space learning capability and the expressions of Drebrin and PSD95 in the hippocampus in APP/PS1 transgenic mouse model of Alzheimer's disease.Methods A total of fifty-four 3-month-old APP/PS1 transgenic mouse were randomly divided into 3 groups:3-month-old control group,sham surgery group,hepatolobectomy group,3-month-old littermates control group and 6-month-old control group.Morris water maze test was used to observe space learning capability on the 1st,3rd,7th,14th day after surgery,meanwhile the expressions of Drebrin and PSD95 in hippocampus were measured by Western blotting.Results Compared with the sham surgery group,the results of the Morris water maze test were decreased in hepatolobectomy group at day 3,7,14 after surgery [(62.9±6.9) s vs.(35.7±12.2) s,(66.3± 9.5) s vs.(39.3±8.3) s,(67.1±7.5) s vs.(32.6±14.1) s],and 6-month-old control group [(75.9±12.1) s] (all P＜0.05).The escape latency were (62.9±6.9)s,(66.3±9.5)s,(67.1± 7.5)s and (75.9±12.1)s,the probe trials were (2.1±0.7) times,(1.83±1.5) times,(2.5±1.9) times and (1.8±0.8) times respectively in hepatolobectomy group at day 3,7,14 after surgery and 6-month old control group.No significant differences in the results of the Morris water maze test were found among 3-month-old control group,3-month-old littermates control group,and sham surgery group.Compared with 3-month-old control group and sham surgery group atday 1,3,7,14 after surgery the expressions of Drebrin were decreased in 6-month-old control group and the hepatolobectomy group at the same time points.Meanwhile,the expression of Drebrin in hepatolobectomy group was increasedat day 14 versus day 7 after surgery.Compared with 3 month-old control group,the expression of Drebrin was increased at day 7 after sham surgery.Compared with 3-month-old control group and sham surgery group at day 3,7 and 14,the expressions of PSD95 were decreased in hepatolobectomy group at the same time points.Compared with 3-month-old control group,the expression of PSD95 was increased in sham surgery group at 7th day (P＜0.05).Between 3 month-old control group and 3-month-old littermates control group,the expressions of Drebrin and PSD95 had no significant differences.Concltsions Hepatolobectomy can impair the capabilities of space learning and memory in 3-month-old APP/PS1 transgenic mice,which may be associated with the decreased expressions of Drebrin and PSD95 in hippocampus.
