CAJ | 학술논문

目的探讨女性压力性尿失禁尿道组织的病理学变化和机制. 方法雌性大鼠分娩后立即行阴道扩张4h,1周后行卵巢摘除构建压力性尿失禁大鼠模型.卵巢切除1个月后分别行清醒下和麻醉下尿流动力学检查.尿道组织取材后进一步行组织化学和Western blot检查. 结果 (1)模型组有85％的大鼠出现压力性尿失禁.(2)模型组尿道组织肌肉含量减少,同时横纹肌出现碎裂,肌纤维排列紊乱.(3)假手术组大鼠横纹肌层弹力纤维与肌纤维连接紧密,纤维连续,而尿失禁大鼠弹力纤维与肌纤维分离,出现碎裂.(4)模型组尿道组织的血管内皮生长因子含量和血管数目下降. 结论雌性压力性尿失禁大鼠尿道的肌肉和弹力纤维出现破坏,VEGF可能是尿道组织形态学改变的重要原因.
목적 탐토녀성압력성뇨실금뇨도조직적병이학변화화궤제. 방법 자성대서분면후립즉행음도확장4h,1주후행란소적제구건압력성뇨실금대서모형.란소절제1개월후분별행청성하화마취하뇨류동역학검사.뇨도조직취재후진일보행조직화학화Western blot검사. 결과 (1)모형조유85％적대서출현압력성뇨실금.(2)모형조뇨도조직기육함량감소,동시횡문기출현쇄렬,기섬유배렬문란.(3)가수술조대서횡문기층탄력섬유여기섬유련접긴밀,섬유련속,이뇨실금대서탄력섬유여기섬유분리,출현쇄렬.(4)모형조뇨도조직적혈관내피생장인자함량화혈관수목하강. 결론 자성압력성뇨실금대서뇨도적기육화탄력섬유출현파배,VEGF가능시뇨도조직형태학개변적중요원인.
Objective To investigate the pathological changes and mechanism in the urethra by parturition-induced stress urinary incontinence.Methods Sprague Dawley female rats underwent vaginal balloon dilation for 4 hours immediately after delivery.One week later,the rats were anesthetized and both ovaries were excised.Then a rat model of stress urinary incontinence (SUI) was successfully established.One month after ovariectomy,conscious cystometry and Leak-Point Pressure (LPP) were measured by MP150.Histological examination and Western blotting were performed after functional assays.Results (1) 85％ of rats presented astress urinary incontinence in the model group.(2) The urethras in SUI rats had decreased muscles,and striated muscles showed fragmentized and disorganized.(3) Elastic fibers were long,well organized and tightly connected to the muscle bundles in sham group,while elastic fibers showed fragmentation and disorganization in the model group.(4) The protein expression of vascular endothelial growth factor (VEGF) and blood vessels were reduced in SUI rats as compared with the sham rats.Conclusions Muscle and elastic fibers in the urethra are disrupted in SUI rat.VEGF may play an important role in regulation of pathological changes in urethra.