中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2012年
9期
883-887
,共5页
陈敏%姚仕堂%马艳玲%何翔%王继宝%程春林%杨锦%苏莹珍%李艳玲%段松%陈会超%付丽茹%贾曼红%陆林
陳敏%姚仕堂%馬豔玲%何翔%王繼寶%程春林%楊錦%囌瑩珍%李豔玲%段鬆%陳會超%付麗茹%賈曼紅%陸林
진민%요사당%마염령%하상%왕계보%정춘림%양금%소형진%리염령%단송%진회초%부려여%가만홍%륙림
人类免疫缺陷病毒-1%亚型%分布特征
人類免疫缺陷病毒-1%亞型%分佈特徵
인류면역결함병독-1%아형%분포특정
HIV-1%Subtype%Distribution characteristics
目的 研究云南省德宏州HIV-1亚型的流行特点.方法 收集2011年1-5月德宏州HIV-1抗体确认阳性血浆样品300份,提取RNA后用巢式PCR对gag基因和env基因的部分片段进行扩增.扩增产物经基因序列测定,所得序列通过构建系统进化树确定HIV-1的分子亚型.结果结合2个基因片段,共有222份样品获得分型结果,主要为C亚型(43 2％,96/222),其他依次为独特重组形式(URF;27.0％,60/222)、CRF01_AE(212％,47/222)、CRF08 BC(5.0％,11/222)、B'(2 3％,5/222)和CRF07 BC( 1.4％,3/222).C亚型是性传播人群和注射吸毒人群中的首要亚型,但不同传播途径人群中亚型分布具有不同的模式.异性传播人群包括该地区检测到6种基因型,主要为C亚型(40 7％,70/172)、CRF 01AE(25.0％,43/172)和多种URF(25.0％,43/172),呈现亚型分布的多样化.注射吸毒人群中包括除B '和CRF07 BC以外的3种亚型及多种URF,主要为C亚型(54.8％,23/42)和URF(38.1％,16/42),呈现亚型相对集中的趋势.URF比例的显著上升成为该地区的另一个流行特点,包括BC重组(41 7％,25/60)和与CRF01 AE相关的重组(58 3％,35/60),这两种URF在性传播和注射吸毒人群中分布的差异无统计学意义.结论 德宏州HIV-1流行株较为复杂,存在5种亚型和多种URF,其中C亚型为主要流行株,不同传播途径亚型分布模式存在差异.
目的 研究雲南省德宏州HIV-1亞型的流行特點.方法 收集2011年1-5月德宏州HIV-1抗體確認暘性血漿樣品300份,提取RNA後用巢式PCR對gag基因和env基因的部分片段進行擴增.擴增產物經基因序列測定,所得序列通過構建繫統進化樹確定HIV-1的分子亞型.結果結閤2箇基因片段,共有222份樣品穫得分型結果,主要為C亞型(43 2％,96/222),其他依次為獨特重組形式(URF;27.0％,60/222)、CRF01_AE(212％,47/222)、CRF08 BC(5.0％,11/222)、B'(2 3％,5/222)和CRF07 BC( 1.4％,3/222).C亞型是性傳播人群和註射吸毒人群中的首要亞型,但不同傳播途徑人群中亞型分佈具有不同的模式.異性傳播人群包括該地區檢測到6種基因型,主要為C亞型(40 7％,70/172)、CRF 01AE(25.0％,43/172)和多種URF(25.0％,43/172),呈現亞型分佈的多樣化.註射吸毒人群中包括除B '和CRF07 BC以外的3種亞型及多種URF,主要為C亞型(54.8％,23/42)和URF(38.1％,16/42),呈現亞型相對集中的趨勢.URF比例的顯著上升成為該地區的另一箇流行特點,包括BC重組(41 7％,25/60)和與CRF01 AE相關的重組(58 3％,35/60),這兩種URF在性傳播和註射吸毒人群中分佈的差異無統計學意義.結論 德宏州HIV-1流行株較為複雜,存在5種亞型和多種URF,其中C亞型為主要流行株,不同傳播途徑亞型分佈模式存在差異.
목적 연구운남성덕굉주HIV-1아형적류행특점.방법 수집2011년1-5월덕굉주HIV-1항체학인양성혈장양품300빈,제취RNA후용소식PCR대gag기인화env기인적부분편단진행확증.확증산물경기인서렬측정,소득서렬통과구건계통진화수학정HIV-1적분자아형.결과결합2개기인편단,공유222빈양품획득분형결과,주요위C아형(43 2％,96/222),기타의차위독특중조형식(URF;27.0％,60/222)、CRF01_AE(212％,47/222)、CRF08 BC(5.0％,11/222)、B'(2 3％,5/222)화CRF07 BC( 1.4％,3/222).C아형시성전파인군화주사흡독인군중적수요아형,단불동전파도경인군중아형분포구유불동적모식.이성전파인군포괄해지구검측도6충기인형,주요위C아형(40 7％,70/172)、CRF 01AE(25.0％,43/172)화다충URF(25.0％,43/172),정현아형분포적다양화.주사흡독인군중포괄제B '화CRF07 BC이외적3충아형급다충URF,주요위C아형(54.8％,23/42)화URF(38.1％,16/42),정현아형상대집중적추세.URF비례적현저상승성위해지구적령일개류행특점,포괄BC중조(41 7％,25/60)화여CRF01 AE상관적중조(58 3％,35/60),저량충URF재성전파화주사흡독인군중분포적차이무통계학의의.결론 덕굉주HIV-1류행주교위복잡,존재5충아형화다충URF,기중C아형위주요류행주,불동전파도경아형분포모식존재차이.
Objective To investigate the distribution of HIV-1 subtypes in Dehong prefecture,Ymnan province,in 2011.Methods 300 HIV-1 positive plasma samples were collected from Jan.2011 to May 2011 in Dehong prefecture.HIV-1 gag genes and env genes were amplified by nestedpolymerase chain reaction (PCR) from viral RNA,After sequencing,the HIV-1 subtypes were determined by phylogenetic analysis.Results Based on the phylogenetic trees of gag gene and env gene fragments,a total of 222 samples were genotyped.Subtype C was the predominant strain in Dehong (43.2％,96/222),followed by unique recombinant forms (URFs,27.0％,60/222),CRF01 _AE ( 21.2％,47/222 ),C R F08_BC ( 5.0％,11/222 ),B,( 2.3％,5/222 ) and CRF07 BC ( 1.4％,3/222).Subtype C strains were predominant in both heterosexually transmitted population and intravenous drug users (IDUs),but different subtype distribution patterns were found in these two populations.All 6 genotypes including subtype C (40.7％,70/172),CRF01_AE (25.0％,43/172),and URFs (25.0％,43/172 ) found in this area among hcterosexually transmitted population,which showed the diversity of genotypes in this population.Except subtype B' and CRF07_BC,the other 3 subtypes and URFs were detected among IDUs,mainly including subtype C (54.8％,23/42) and U R Fs (38.1％,16/42),vhich shoved the concentration trend of genotypes distribution among IDUs.The proportion of URFs increased significantly in this area,including the new BC recombinants (41.7％,25/60) and CRF01_AE relative URFs (58.3％,35/60).However,the distributions of these two URFs among heterosexually transmitted population and IDUs showed no statistical significance.Conclusion The distribution of HIV-1 strains prevailing in Dehong prefecture was diversity,including 5 subtypes and a variety of URFs,of which subtype C was the predominant strain.The distribution patterns of subtype were different among different populations.
