CAJ | 학술논문

目的分析郑州市男男性行为者(MSM)中HIV感染者的毒株变异特点及其与当地有偿供血者中流行毒株的关系.方法以郑州市2010年确证的31例MSM的HIV感染者和41例既往有偿供血HIV感染者为研究对象,采集血标本并收集流行病学信息.RT-PCR法扩增全部研究对象的HIV-1 gag、pol全长基因(分别为1584 bp和3147 bp)及env基因的C2V3片段(558 bp)并测序,使用LosAlamosHIV Database在线工具确定病毒基因亚型、绘制系统进化树并分析可能的传播关系.结果在72例研究对象中,获得全长gag序列53条、pol序列38条、env(C2V3)序列48条；31例MSM的HIV感染者中,CRF01 AE亚型14例、CRF07_BC亚型5例、B亚型12例(B'亚型11例、B亚型1例),41例既往有偿供血的HIV感染者均为B'亚型.MSM人群的CRF01 AE亚型毒株与河北、辽宁省的毒株关系密切、CRF07 BC毒株与河北省石家庄和北京市的毒株关系密切.在MSM人群中的12条HIV-1 B亚型中,有8条聚集成簇并与1例有偿供血感染者的序列距离最近、2条形成可靠的进化簇且与02HNseq4的序列距离最近,提示MSM人群中的B'毒株可能来自有偿供血员,且在MSM人群中存在传播关系.结论郑州市MSM人群中流行的HIV-1毒株遗传背景复杂、有多个来源.首次发现目前MSM人群中流行的HIV-1 B亚型毒株主要是B'亚型,可能来自当地的有偿供血人群.
목적 분석정주시남남성행위자(MSM)중HIV감염자적독주변이특점급기여당지유상공혈자중류행독주적관계.방법 이정주시2010년학증적31례MSM적HIV감염자화41례기왕유상공혈HIV감염자위연구대상,채집혈표본병수집류행병학신식.RT-PCR법확증전부연구대상적HIV-1 gag、pol전장기인(분별위1584 bp화3147 bp)급env기인적C2V3편단(558 bp)병측서,사용LosAlamosHIV Database재선공구학정병독기인아형、회제계통진화수병분석가능적전파관계.결과 재72례연구대상중,획득전장gag서렬53조、pol서렬38조、env(C2V3)서렬48조；31례MSM적HIV감염자중,CRF01 AE아형14례、CRF07_BC아형5례、B아형12례(B'아형11례、B아형1례),41례기왕유상공혈적HIV감염자균위B'아형.MSM인군적CRF01 AE아형독주여하북、요녕성적독주관계밀절、CRF07 BC독주여하북성석가장화북경시적독주관계밀절.재MSM인군중적12조HIV-1 B아형중,유8조취집성족병여1례유상공혈감염자적서렬거리최근、2조형성가고적진화족차여02HNseq4적서렬거리최근,제시MSM인군중적B'독주가능래자유상공혈원,차재MSM인군중존재전파관계.결론 정주시MSM인군중류행적HIV-1독주유전배경복잡、유다개래원.수차발현목전MSM인군중류행적HIV-1 B아형독주주요시B'아형,가능래자당지적유상공혈인군.
Objective To clarify the genetic characteristics of human immunodeficiency virus (HIV) circulated in the population of men who have sex with men (MSM) in Zhengzhou,Henan and to analyze its relationship with HIV-1 prevailing in the paid blood donors (PBDs).Methods Thirty-one MSM who were confirmed as HIV positive individuals in 2010 together with 41 HIV-positive former PBDs were enrolled in the study.Information on related epidemiological characteristics and their plasma were collected.RT-PCR was used to amplify HIV-1 full length gag (1584 bp),pol (3147 bp) genes and partial env gene (C2V3 segment,558 bp) followed by sequencing on those subjects.Online software available at LosAlamos HIV Database was used to identify the HIV subtypes based on the findings of the sequences.Phylogenetic tree was used to identify thc possible relationship of transmission.Results Fifty-three full length gag,38 full length pol and 48 partial env (C2V3) genes were collected from 72 participants.Among the 31 HIV ( + )MSM individuals,14 CRF01_AE strains,5 CRF07BC_ strains and 12 subiype B ( 1 subtype B and 11B' ) strains were identified respectively.All of the 41 strains identified from former PBDs were infected by B' strains.The CRF01_AE strains identified in MSM showed a close relationship to those identified from both Hebei and Liaoning provinces.The CRF07 BC strains showed a close relationship with those from Shijiazhuang and Beijing cities.Among the 12 subtype B strains,8 sequences grouped into 1 cluster with 1 sequence from the former PBDs.Two sequences grouped with 02HNseq4 suggested that B' had been prevailed in the MSM population might come from the former PBDs and were closely related to the strains identified in the MSM population.Conclusion Complicated genetic background and multiple introductions of HIV in the MS population in Zhengzhou,were found.This was also the first report which noticed that the subtype B epidemic among Zhengzhou MSM was mainly originated from the B' among the former PBDs.