CAJ | 학술논문

目的探讨江苏地区高危人群IL-10基因多态性与丙型肝炎病毒(HCV)感染转归的关系.方法运用Taqman-MGB技术检测264例HCV自限感染者、371例HCV持续感染者和920例对照者的IL-10-819T/C、-592A/C、- 1082A/G位点基因多态性.结果调整性别、年龄和高危人群种类混杂因素后,logistic回归分析显示3个位点的基因多态性分别与HCV感染转归无显著关联(P值均＞0.05).进一步分层分析显示,-819T/C位点中,TC基因型使中年人、女性和有偿献血人群HCV自限感染的机会增加[调整OR值及其95％CI分别为2.160( 1.163 ～ 4.011)、1.693( 1.066～ 2.688)和4.084( 1.743～9.570)],在有偿献血人群中使个体进展为持续感染的风险降低(调整OR=0.312,95％CI:0.130 ～ 0.747)；CC基因型使血液透析人群HCV自限感染的机会增加(调整OR=2.120,95％CI:1071～4.197),同时在有偿献血人群中使个体进展为持续感染的风险降低(调整OR=0.156,95％CI:0.043 ～ 0.566).-592A/C位点中,AC基因型使中年人、女性和有偿献血人群HCV自限感染的机会显著增加[调整OR值及其95％CI分别为2.176(1.173 ～4.037)、1.659( 1.055 ～ 2.607)和3.704( 1.625 ～ 8.443)],在女性中增加了HCV持续感染的风险(调整OR=1.525,95％CI:1.017～ 2.286),在吸毒人群中使个体进展为持续感染的风险增加(调整OR=1.845和95％CI:1.122～3.034),而在有偿献血人群中使个体进展为持续感染的风险降低(调整OR=0.361,95％CI:0.155 ～ 0.841).CC基因型增加了有偿献血人群HCV自限感染的机会,同时也使个体进展为持续感染的风险降低[调整OR值及其95％CI分别为3.125(1.016 ～ 9.605)和0.218( 0.063～0.748)].-1082A/G位点中,突变基因型AG/GG能增加有偿献血人群HCV自限感染的机会(调整OR=3.780,95％CI:1.620 ～ 8.82 0).结论 IL-10-819T/C、-592A/C、-1082A/G三个位点的基因多态性在不同高危人群中与HCV感染转归可能有一定关联.
목적 탐토강소지구고위인군IL-10기인다태성여병형간염병독(HCV)감염전귀적관계.방법 운용Taqman-MGB기술검측264례HCV자한감염자、371례HCV지속감염자화920례대조자적IL-10-819T/C、-592A/C、- 1082A/G위점기인다태성.결과 조정성별、년령화고위인군충류혼잡인소후,logistic회귀분석현시3개위점적기인다태성분별여HCV감염전귀무현저관련(P치균＞0.05).진일보분층분석현시,-819T/C위점중,TC기인형사중년인、녀성화유상헌혈인군HCV자한감염적궤회증가[조정OR치급기95％CI분별위2.160( 1.163 ～ 4.011)、1.693( 1.066～ 2.688)화4.084( 1.743～9.570)],재유상헌혈인군중사개체진전위지속감염적풍험강저(조정OR=0.312,95％CI:0.130 ～ 0.747)；CC기인형사혈액투석인군HCV자한감염적궤회증가(조정OR=2.120,95％CI:1071～4.197),동시재유상헌혈인군중사개체진전위지속감염적풍험강저(조정OR=0.156,95％CI:0.043 ～ 0.566).-592A/C위점중,AC기인형사중년인、녀성화유상헌혈인군HCV자한감염적궤회현저증가[조정OR치급기95％CI분별위2.176(1.173 ～4.037)、1.659( 1.055 ～ 2.607)화3.704( 1.625 ～ 8.443)],재녀성중증가료HCV지속감염적풍험(조정OR=1.525,95％CI:1.017～ 2.286),재흡독인군중사개체진전위지속감염적풍험증가(조정OR=1.845화95％CI:1.122～3.034),이재유상헌혈인군중사개체진전위지속감염적풍험강저(조정OR=0.361,95％CI:0.155 ～ 0.841).CC기인형증가료유상헌혈인군HCV자한감염적궤회,동시야사개체진전위지속감염적풍험강저[조정OR치급기95％CI분별위3.125(1.016 ～ 9.605)화0.218( 0.063～0.748)].-1082A/G위점중,돌변기인형AG/GG능증가유상헌혈인군HCV자한감염적궤회(조정OR=3.780,95％CI:1.620 ～ 8.82 0).결론 IL-10-819T/C、-592A/C、-1082A/G삼개위점적기인다태성재불동고위인군중여HCV감염전귀가능유일정관련.
Objective To explore the relationship between interleukin (IL)-10 gene polymorphisms and the susceptibility or the outcomes of HCV infection among high-risk populations in Jiangsu province.Methods IL-10 gene SNPs were detected in 1555 subjects including 264 self-limited HCV infections.371 persistent HCV infections and 920 healthy controls were selected through Taqman-MGB.Results After adjusted for cofounders as sex,age and high-risk population,data from logistic regression analysis showed that the distribution of IL-10 genotypes among the controls,spontaneous clearances and those with persistent infections did not show much differences.Results from further stratified analysis showed that,at the position of-819T/C,when compared with TT genotype,TC genotype had a significantly increasing chance of self-limited HCV infection among middle-aged,females and paid blood doners (adjusted OR values and 95％ CI were:2.160,1.163 4.011 ；1.693,1.066-2.688 and 4.084,1.743-9.570).It also had a lower risk of progressing to persistent HCV infection among those paid blood doners (the adjusted OR values and 95％CI were:0.312,0.130-0.747 ).CC genotype had a higher chance of self-limited HCV infection among people underwent blood dialysis (the adjusted OR values and 95％CI were:2.120,1.071 -4.197).Results also showed a decreased risk of progressing to persistent infection among paid blood doners (the adjusted OR values and 95％CI were:0.156,0.043-0.566).At the position of -592A/C,when compared to AA genotypc,the AC genotype had a significantly increasing chance of self-limited HCV infection among middle-aged,femalcs and paid blood doners (the adjusted OR values and 95％ CI were:2.176,1.173-4.037；1.659,1.055-2.607;3.704,1.625-8.443) but had an increased risk of persistent HCV infection among females (the adjusted OR values and 95％CI were:1.525,1.017-2.286).AC genotype showed an increased opportunity to progress to HCV persistent infection among drug users (the adjusted OR values and 95％CI were:1.845,1.122-3.034) but had a reduced risk of progressing to HCV persistent infection among paid blood doners (the adjusted OR values and 95％CI were:0.361,0.155-0.841 ).CC genotype had an increased opportunity to self-linited HCV infection as well as having a dccreased risk of progressing to persistent infection among paid blood donets (the adjusted OR values and 95％CI were:3.125,1.016-9.605；0.218,0.063-0.748).At the position of-1082A/G,AG/GG genotypcs had an increased chance of self-limited infection among blood doners (the adjusted OR values and 95％CI were:3.780,1.620-8.820).Conclusion IL-10-819T/C,-592A/C,-1082A/G SNPs might be related with the susceptibility and the outcomes of HCV infection among populations at high risk.