CAJ | 학술논문

目的了解2010年北京市手足 (口)重症病例肠道病毒(EV)检出情况及EV71病原学特征.方法荧光定量RT-PCR检测EV71和柯萨奇病毒A组16型(CoxA16).采用RD细胞对检测阳性的咽拭子进行病毒分离培养和鉴定,对EV71分离株的VP1基因序列进行种系发生分析.结果2010年北京市手足口重症病例442例荧光定量RT-PCR检测阳性253例,阳性检出率为57.24％,其中EV71为54.55％ (138/253),CoxA16为5.93％(15/253),其他肠道病毒为39.53％( 100/253).VP1基因序列分析表明2010年北京市手足口重症病例的12株EV71分离株均属于C4a基因型,核苷酸同源性为97.2％～ 100.0％；与2007-2010年北京EV71分离株、2007年山东手足重症病例、2008年安徽阜阳及2008年广东手足口重症及死亡病例EV71分离株VP1基因序列核苷酸同源性为94.0％～ 99.9％.结论北京市2010年手足口重症病例由C4a基因型EV71引起,其中至少有4个病毒链在重症病例发病中起作用.分离的12株EV71与安徽阜阳地区2008年手足口重症病例分离株亲缘关系较近.
목적 료해2010년북경시수족 (구)중증병례장도병독(EV)검출정황급EV71병원학특정.방법 형광정량RT-PCR검측EV71화가살기병독A조16형(CoxA16).채용RD세포대검측양성적인식자진행병독분리배양화감정,대EV71분리주적VP1기인서렬진행충계발생분석.결과2010년북경시수족구중증병례442례형광정량RT-PCR검측양성253례,양성검출솔위57.24％,기중EV71위54.55％ (138/253),CoxA16위5.93％(15/253),기타장도병독위39.53％( 100/253).VP1기인서렬분석표명2010년북경시수족구중증병례적12주EV71분리주균속우C4a기인형,핵감산동원성위97.2％～ 100.0％；여2007-2010년북경EV71분리주、2007년산동수족중증병례、2008년안휘부양급2008년엄동수족구중증급사망병례EV71분리주VP1기인서렬핵감산동원성위94.0％～ 99.9％.결론 북경시2010년수족구중증병례유C4a기인형EV71인기,기중지소유4개병독련재중증병례발병중기작용.분리적12주EV71여안휘부양지구2008년수족구중증병례분리주친연관계교근.
Objective To study the etiological detection on samples from severe hand-footmouth disease (HFMD) cases and the genetic characteristics of enterovirus type 71 (EV71) isolates lrom severe patients in Beijing,2010.Methods Real-time RT-PCR was used to detect EV71 and Coxsackievirus A16 (CoxA16) and RD cells were used to separate virus strains from samples.Homogeneity of EV71 isolated strains were also analyzed. Results Four hundred and fourty-two severe cases were detected and 253 were positive,taking up 57.24％ of the total (253/442).The overall positive detection rate on EV71 was 54.55％ (138/253),with CoxA16 as 5.93％(15/253),and with other enterovirus group was 39.53％(100/253).The nucleotide homogencity of VP1 within these 12 strains was 97.2％ 100.0％,and with Beijing strains in 2007-2010,Shandong strains in 2007 and Anhui Fuyang strains in 2008 and the Guangdong strains in 2008 as 94.0％-99.9％.Conclusion Severe HFMD cases were most oftenly caused by EV71 but less caused by CoxA16 or other cnterovirus.The HFMD in 2010 in Beijing was mainly caused by EV71 subgenotype C4a with 4 transmission chains.Twclve isolated EV71 strains had high homogeneity with strains isolated from severe cases in Anhui Fuyang in 2007.