中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2012年
11期
1167-1170
,共4页
梁庆福%陈求扬%林淑芳%林建%逄宇%赵永%魏淑贞%王玉锋%郑金凤%赵雁林
樑慶福%陳求颺%林淑芳%林建%逄宇%趙永%魏淑貞%王玉鋒%鄭金鳳%趙雁林
량경복%진구양%림숙방%림건%방우%조영%위숙정%왕옥봉%정금봉%조안림
结核分枝杆菌%多位点可变数目串联重复序列%基因分型
結覈分枝桿菌%多位點可變數目串聯重複序列%基因分型
결핵분지간균%다위점가변수목천련중복서렬%기인분형
Mycobacterium tuberculosis%Multiple loci VNTR analysis%Genotyping
目的 了解福建省结核分枝杆菌的多位点可变数目串联重复序列基因分型(MLVA)的特征.方法 选择15个可变数目串联重复位点(VNTR),检测福建省30个耐药监测点临床分离的结核菌株,结果使用BioNumerics (Version 4.5)软件进行聚类分析.结果 313株结核菌被分为9个基因群(Ⅰ～Ⅸ),分别包含220、9、48、2、1、3、10、10、10株菌,以Ⅰ群为主(70.3％,220/313);Ⅰ群菌株异烟肼、链霉素、乙胺丁醇和耐多药的耐药率与其他基因群的差异无统计学意义(P＞0.05),但利福平(RFP)耐药率为33.2％(73/220),明显高于其他群菌株RFP的耐药率20.4％(19/93),差异有统计学意义(P＜0.05).结论 福建省结核分枝杆菌菌株存在明显的基因多态性,以Ⅰ群菌株为主,并与RFP耐药性具有相关性,应加强此类菌株流行的监测.
目的 瞭解福建省結覈分枝桿菌的多位點可變數目串聯重複序列基因分型(MLVA)的特徵.方法 選擇15箇可變數目串聯重複位點(VNTR),檢測福建省30箇耐藥鑑測點臨床分離的結覈菌株,結果使用BioNumerics (Version 4.5)軟件進行聚類分析.結果 313株結覈菌被分為9箇基因群(Ⅰ～Ⅸ),分彆包含220、9、48、2、1、3、10、10、10株菌,以Ⅰ群為主(70.3％,220/313);Ⅰ群菌株異煙肼、鏈黴素、乙胺丁醇和耐多藥的耐藥率與其他基因群的差異無統計學意義(P＞0.05),但利福平(RFP)耐藥率為33.2％(73/220),明顯高于其他群菌株RFP的耐藥率20.4％(19/93),差異有統計學意義(P＜0.05).結論 福建省結覈分枝桿菌菌株存在明顯的基因多態性,以Ⅰ群菌株為主,併與RFP耐藥性具有相關性,應加彊此類菌株流行的鑑測.
목적 료해복건성결핵분지간균적다위점가변수목천련중복서렬기인분형(MLVA)적특정.방법 선택15개가변수목천련중복위점(VNTR),검측복건성30개내약감측점림상분리적결핵균주,결과사용BioNumerics (Version 4.5)연건진행취류분석.결과 313주결핵균피분위9개기인군(Ⅰ～Ⅸ),분별포함220、9、48、2、1、3、10、10、10주균,이Ⅰ군위주(70.3％,220/313);Ⅰ군균주이연정、련매소、을알정순화내다약적내약솔여기타기인군적차이무통계학의의(P＞0.05),단리복평(RFP)내약솔위33.2％(73/220),명현고우기타군균주RFP적내약솔20.4％(19/93),차이유통계학의의(P＜0.05).결론 복건성결핵분지간균균주존재명현적기인다태성,이Ⅰ군균주위주,병여RFP내약성구유상관성,응가강차류균주류행적감측.
Objective To preliminarily understand the genotyping characteristics regarding the variable number tandem repeats (VNTR) of Mycobacterium tuberculosis clinical isolates so as to provide evidence for the development of tuberculosis control and prevention programs in Fujian province.Methods Fifteen VNTR locus sets were used to detect the clinical isolates from the fifth surveillance project on tuberculosis resistance,in Fujian province.BioNumerics version 4.5 were used to analyze the cluster from the results generated by genotyping.Results 313 Mycobacterium tuberculosis isolates were divided into 9 clusters,including Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ,Ⅶ,Ⅷ and Ⅸ,with the number of 220,9,48,2,1,3,10,10,10 isolates,respectively.Cluster Ⅰ was the major lineage,accounting for 70.3％ (220/313) of the total.Resistance rates of cluster Ⅰ isolates to isoniazid,streptomycin,ethambutol and multi-drug-resistaut were not statistically different from other clusters (P＞0.05).However,resistance rate to rifampicin (RFP) was significantly higher than that of other isolates of the clusters,33.2％ (73/220) vs.20.4％ (19/93) (P＜0.05).Conclusion The strains isolated from Fujian province showed significant polymorphism on genotyping.Cluster Ⅰ seemed to be the dominant,calling for the close monitoring program on cluster Ⅰ strains.Results from our initial studies demonstrated the existence of significant correlation between cluster Ⅰ strains and drug resistance to RFP.
