CAJ | 학술논문

目的分析2009年浙江省随访艾滋病病毒感染者/艾滋病患者(HIV/AIDS)分子流行病学特征.方法以浙江省各地市为单位随机抽样确定303例研究对象,收集个案信息、采集样本并提取基因组DNA,用巢式PCR扩增gag基因片段,测序并进行生物信息学分析.结果序列成功获得率为74.3％(225/303).存在7种亚型,分别为CRF01_ AE (58.7％)、CRF07_ BC(13.8％)、CRF08_BC(9.8％)、B'(15.1％)、C(1.8％)、G(0.4％)和未定义BC重组(新型独特重组型,0.4％).HIV BLAST分析显示,毒株同源性最高的报告地涉及国内辽宁、广西、云南、河南等10个省(区)和泰国、越南、印度、南非和利比亚5个国家.CRF01 AE系统进化树分为4簇,同性传播感染者序列聚集于簇l,但与异性传播感染者混合并存.结论浙江省HIV毒株的多样性和复杂性进一步增加.以流行重组型占显著优势,存在新型独特重组型和新型别,同性传播与异性传播感染者呈交叉渗透趋势.
목적 분석2009년절강성수방애자병병독감염자/애자병환자(HIV/AIDS)분자류행병학특정.방법 이절강성각지시위단위수궤추양학정303례연구대상,수집개안신식、채집양본병제취기인조DNA,용소식PCR확증gag기인편단,측서병진행생물신식학분석.결과 서렬성공획득솔위74.3％(225/303).존재7충아형,분별위CRF01_ AE (58.7％)、CRF07_ BC(13.8％)、CRF08_BC(9.8％)、B'(15.1％)、C(1.8％)、G(0.4％)화미정의BC중조(신형독특중조형,0.4％).HIV BLAST분석현시,독주동원성최고적보고지섭급국내료녕、엄서、운남、하남등10개성(구)화태국、월남、인도、남비화리비아5개국가.CRF01 AE계통진화수분위4족,동성전파감염자서렬취집우족l,단여이성전파감염자혼합병존.결론 절강성HIV독주적다양성화복잡성진일보증가.이류행중조형점현저우세,존재신형독특중조형화신형별,동성전파여이성전파감염자정교차삼투추세.
Objective To analyze the molecular epidemiological characteristics on HIV infectors/AIDS patients (HIV/AIDS) under a follow-up program in Zhejiang province in 2009.Methods 303 cases were randomly sampled.Information on the cases was collected and followed by genomic DNA extraction.Gag gene fragments were amplified by nested PCR,followed by sequencing and bio-informatic analysis.Results The rate of success for sequence acquisition was 74.3％ (225/303).Distributions of HIV subtypes were as follows:CRF01_AE (58.7％),CRF07_BC (13.8％),CRF08_BC (9.8％),B' (15.1％),C (1.8％),G (0.4％) and unassigned BC (unique recombinant form 0.4％).Results from the HIV BLAST analysis showed that the sources of strains with the highest homology involved in 10 provinces/municipalities (Liaoning,Guangxi,Yunnan,Henan,etc.) and five other countries (Thailand,Vietnam,India,South Africa and Libya).The CRF01 _AE phylogenetic tree was divided into four clusters.The sequences of HIV/AIDS with homosexual transmission showed a gathering in cluster 1,and mixing with those infected through heterosexual contact.Conclusion Circulating recombinant forms of HIV seemed to play a dominant role in Zhejiang province.Unique recombinant form and new subtype of HIV were found.People living with HIV under homosexual transmission and heterosexual transmission had a trend of interwoven with each other.Increase of both the diversity and complexity of HIV strains were also noticed in Zhejiang province.