中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2013年
2期
183-186
,共4页
陈占国%周武%戴美洁%武志刚%金嵘
陳佔國%週武%戴美潔%武誌剛%金嶸
진점국%주무%대미길%무지강%금영
膀胱癌%白介素10%多态性%吸烟
膀胱癌%白介素10%多態性%吸煙
방광암%백개소10%다태성%흡연
Bladder neoplasia%Interleukin 10%Polymorphism%Smoking
目的 探讨IL-10基因启动子区单核苷酸多态性和吸烟的交互作用与膀胱癌的关系.方法 以医院资料为基础,采用病例对照研究方法,应用AS-PCR法对400例膀胱癌病例和400例健康对照者进行IL-10基因启动子区单核苷酸多态性检测和分析,探讨膀胱癌的遗传和环境因素.结果 IL-10基因启动子区单核苷酸多态性与膀胱癌易感性相关,-1082、-819及-592位点的纯合突变型可能增加膀胱癌发病风险(OR值分别为2.058、1.979及1.979)；IL-10基因启动子区单核苷酸多态性在膀胱癌不同临床分期和病理分级间差异无统计学意义(均P＞0.05);IL-10基因启动子区-1082A/A、-819T/T和-592A/A与吸烟均存在正向交互作用(OR=2.264,γ=10.213；OR=2.438,γ=6.750; OR=2.438,γ=6.750).结论 IL-10基因启动子区-1082A/A、-819T/T和-592A/A与吸烟的交互作用可能增加膀胱癌的发病风险.
目的 探討IL-10基因啟動子區單覈苷痠多態性和吸煙的交互作用與膀胱癌的關繫.方法 以醫院資料為基礎,採用病例對照研究方法,應用AS-PCR法對400例膀胱癌病例和400例健康對照者進行IL-10基因啟動子區單覈苷痠多態性檢測和分析,探討膀胱癌的遺傳和環境因素.結果 IL-10基因啟動子區單覈苷痠多態性與膀胱癌易感性相關,-1082、-819及-592位點的純閤突變型可能增加膀胱癌髮病風險(OR值分彆為2.058、1.979及1.979)；IL-10基因啟動子區單覈苷痠多態性在膀胱癌不同臨床分期和病理分級間差異無統計學意義(均P＞0.05);IL-10基因啟動子區-1082A/A、-819T/T和-592A/A與吸煙均存在正嚮交互作用(OR=2.264,γ=10.213；OR=2.438,γ=6.750; OR=2.438,γ=6.750).結論 IL-10基因啟動子區-1082A/A、-819T/T和-592A/A與吸煙的交互作用可能增加膀胱癌的髮病風險.
목적 탐토IL-10기인계동자구단핵감산다태성화흡연적교호작용여방광암적관계.방법 이의원자료위기출,채용병례대조연구방법,응용AS-PCR법대400례방광암병례화400례건강대조자진행IL-10기인계동자구단핵감산다태성검측화분석,탐토방광암적유전화배경인소.결과 IL-10기인계동자구단핵감산다태성여방광암역감성상관,-1082、-819급-592위점적순합돌변형가능증가방광암발병풍험(OR치분별위2.058、1.979급1.979)；IL-10기인계동자구단핵감산다태성재방광암불동림상분기화병리분급간차이무통계학의의(균P＞0.05);IL-10기인계동자구-1082A/A、-819T/T화-592A/A여흡연균존재정향교호작용(OR=2.264,γ=10.213；OR=2.438,γ=6.750; OR=2.438,γ=6.750).결론 IL-10기인계동자구-1082A/A、-819T/T화-592A/A여흡연적교호작용가능증가방광암적발병풍험.
Objective To investigate the relationship between both polymorphisms of interleukin-10 (IL-10),smoking and the susceptibility to bladder cancer.Methods A case-control study was conducted to study the promoter polymorphisms of IL-10 gene by allele specific PCR amplification (AS-PCR) and to explore the possible genetic and environmental factors on bladder cancer,based on data from a hospital which included 400 patients with bladder cancer and another 400 healthy controls.Results The genotypes of IL-10 gene might be associated with the susceptibility to bladder cancer.Homozygous mutant of IL-10 gene at the point of 1082,819 and 592 could enhance the risk of bladder cancer (OR value is 2.058,1.979,1.979,respectively).No statistically significant correlation was found between the divergence of IL-10 genotype and the different clinical stages and pathological grade of bladder cancer (P＞0.05).Interactions were noticed between polymorphisms in IL-10 gene and their correlation with smoking on bladder cancer.The positive interaction of 1082 site homozygous variant (AA),819 site homozygous variant (TT),592 site homozygous variant (AA) and smoking were revealed in the occurrence rates of bladder cancer (OR=2.264,γ=l0.213;OR=2.438,γ=6.750; OR=2.438,y=6.750).Conclusion Our research findings showed that the significant interactions between IL-10 gene with homozygous mutant and smoking might increase the risk of bladder cancer.
