CAJ | 학술논문

目的调查云南中缅边境一起输入性登革热流行状况及其流行病毒株的分子流行病学特点.方法采集医院就诊和口岸入境人员中登革热、疑似登革热和不明原因发热患者血清标本并进行流行病学调查,采用ELISA检测登革病毒IgM抗体,RT-PCR检测登革病毒核酸,核酸阳性标本进行登革病毒PrM-C和NS5区的基因核苷酸序列测定和分析.结果 2008年7-11月在云南省瑞丽市共采集急性期患者血清标本103份,经登革病毒IgM抗体和核酸检测,49例确诊为登革热.其中除1例为当地感染病例,其余48例均为输入性病例,其中18例来自缅甸木姐市居民,30例为中国居民到缅甸经商或务工返回后发病者.从缅甸输入病例血清中获得2株病毒(RLB61和RLC31)的PrM-C和NS5区基因核苷酸序列,同源性和系统进化分析表明,RLB61株为登革1型病毒,RLC31株为登革3型病毒,与东南亚登革病毒流行株具有较近的亲缘关系.结论经血清学和分子流行病学证实瑞丽市边境地区发生输入性登革热暴发,并间接证实缅甸木姐市2008年存在登革1和3型病毒流行.
목적 조사운남중면변경일기수입성등혁열류행상황급기류행병독주적분자류행병학특점.방법 채집의원취진화구안입경인원중등혁열、의사등혁열화불명원인발열환자혈청표본병진행류행병학조사,채용ELISA검측등혁병독IgM항체,RT-PCR검측등혁병독핵산,핵산양성표본진행등혁병독PrM-C화NS5구적기인핵감산서렬측정화분석.결과 2008년7-11월재운남성서려시공채집급성기환자혈청표본103빈,경등혁병독IgM항체화핵산검측,49례학진위등혁열.기중제1례위당지감염병례,기여48례균위수입성병례,기중18례래자면전목저시거민,30례위중국거민도면전경상혹무공반회후발병자.종면전수입병례혈청중획득2주병독(RLB61화RLC31)적PrM-C화NS5구기인핵감산서렬,동원성화계통진화분석표명,RLB61주위등혁1형병독,RLC31주위등혁3형병독,여동남아등혁병독류행주구유교근적친연관계.결론 경혈청학화분자류행병학증실서려시변경지구발생수입성등혁열폭발,병간접증실면전목저시2008년존재등혁1화3형병독류행.
Objective To understand the epidemiologic characteristics of dengue fever,imported from Myanmar to the border of Yunnan province,China.Viral molecular epidemiologic features were also studied.Methods Questionnaires were used on each diagnosed,suspected dengue fever,case or unknown cases with fever when coming from Myanmar entering the port and hospitals in Ruili city of Yunnan province.Serum samples of these patients were collected to detect IgM antibody against dengue virus and RT-PCR assay.Homology and phylogenetic tree based on the whole nucleotide sequence of PrM-C and NS5 gene of dengue virus were further analyzed.Results A total of 103 sera were collected from patients at acute stage in Ruili city in July to November 2008.Among them,49 cases were confirmed for dengue fever according to IgM and nucleic acid testings.Except one,other 48 cases were all imported into Ruili,from Myanmar.Of those,18 patients were residents from Mujie city of Myanmar and hospitalized in Ruili and the rest 30 patients were Chinese citizens who had finished business and returned from Myanmar.Two isolates of serum samples from the imported cases were identified and both homology and phylogenetic analysis were performed,using the nucleotide sequences of PrM and NS5 genes.They were divided into dengue type 1 (RLB61) and dengue type 3 (RLC31) and were closer to the dengue virus strains isolated from Southeast Asia countries.Conclusion It is confirmed that an epidemic of dengue fever which was imported from Myanmar to Ruili city of Yunnan province,China.Evidence also showed that both type Ⅰ and Ⅲ epidemic strains of dengue virus did exist in Mujie city of Myanmar in 2008.