CAJ | 학술논문

目的通过PulseNet China数据库对安徽省2012年4起霍乱疫情进行溯源分析.方法应用PCR扩增霍乱弧菌CT和TCP毒力基因,并利用脉冲场凝胶电泳(PFGE)对其进行分型,使用BioNumericsV4.0软件对电泳结果进行聚类分析,并与PulseNet China数据库进行比对.结果 16株菌经PCR扩增,CT和TCP基因均出现目的条带.菌株经Not Ⅰ和SfiⅠ酶切,PFGE聚类分析显示,13株菌的同源性＞98％.其中2株菌与PulseNet China数据库中湖南、四川、浙江、上海、湖北5省(市)同年霍乱疫情菌株同源性达100％.结论安徽省2012年4起霍乱疫情均源自食用霍乱弧菌感染甲鱼.PulseNet China数据库能有效、快速地对霍乱疫情溯源和预警.
목적 통과PulseNet China수거고대안휘성2012년4기곽란역정진행소원분석.방법 응용PCR확증곽란호균CT화TCP독력기인,병이용맥충장응효전영(PFGE)대기진행분형,사용BioNumericsV4.0연건대전영결과진행취류분석,병여PulseNet China수거고진행비대.결과 16주균경PCR확증,CT화TCP기인균출현목적조대.균주경Not Ⅰ화SfiⅠ매절,PFGE취류분석현시,13주균적동원성＞98％.기중2주균여PulseNet China수거고중호남、사천、절강、상해、호북5성(시)동년곽란역정균주동원성체100％.결론 안휘성2012년4기곽란역정균원자식용곽란호균감염갑어.PulseNet China수거고능유효、쾌속지대곽란역정소원화예경.
Objective To track the source of infection regarding 4 Cholerae outbreaks in Anhui province in 2012 through the applicaton of PulseNet China Database (PNCD).Methods Cholerae virulence genes were amplified by PCR and typed by pulse field gel electrophoresis(PFGE).Results from electrophoresis were cluster-analyzed by BioNumericsV4.0 software and compared with PNCD.Results Virulence gene CT and TCP of the tested vibrio cholera showed both positive.Homology of the strains from four cholera outbreaks was more than 98％,based on the homologious and cluster analysis through enzyme digested PFGE electrophoresis.Those strains were highly homologous with the cholera epidemic strains identified in Hunan,Sichuan,Zhejiang,Shanghai and Hubei by PNCD,with the homology as 100％.Conclusion Four chorela outbreaks in Anhui province,2012 were highly correlated with the outbreaks occurring in Hunan and Sichuan during the same time period,indicating that PNCD could effectively and quickly tracking down the source of infection on the cholera outbreaks and providing early warning of the situation.