CAJ | 학술논문

目的利用＜l岁临床百日咳疑似病例实验室检测结果,分析比较不同检测方法.方法采集2011年12月至2012年12月西安市儿童医院＜1岁百日咳临床疑似病例的鼻咽拭子及血清标本,采用分离培养、IS481 PCR及百日咳毒素(PT)IgG定量酶联免疫吸附试验(PT-IgG)检测百日咳鲍特菌感染,并应用SPSS 16.0软件分析结果.结果 148例临床疑似病例中实验室诊断病例100例,其中3例百日咳鲍特菌分离培养阳性,88例IS481 PCR阳性,34例PT-IgG阳性,IS481 PCR和PT-IgG均阳性22例.IS481 PCR及PT-IgG检测结果与患儿发病天数的差异有统计学意义(P＜0.01).结论百日咳鲍特菌分离培养方法敏感度较低,而采用IS481 PCR和PT-IgG两种方法还应结合发病天数加以判断.
목적 이용＜l세림상백일해의사병례실험실검측결과,분석비교불동검측방법.방법 채집2011년12월지2012년12월서안시인동의원＜1세백일해림상의사병례적비인식자급혈청표본,채용분리배양、IS481 PCR급백일해독소(PT)IgG정량매련면역흡부시험(PT-IgG)검측백일해포특균감염,병응용SPSS 16.0연건분석결과.결과 148례림상의사병례중실험실진단병례100례,기중3례백일해포특균분리배양양성,88례IS481 PCR양성,34례PT-IgG양성,IS481 PCR화PT-IgG균양성22례.IS481 PCR급PT-IgG검측결과여환인발병천수적차이유통계학의의(P＜0.01).결론 백일해포특균분리배양방법민감도교저,이채용IS481 PCR화PT-IgG량충방법환응결합발병천수가이판단.
Objective To confirm the clinically suspected pertussis cases (＜ 1 years old) through laboratory methods.Methods From December,2011 to December,2012,patients with clinically suspected pertussis from Xi'an Children's Hospital were sampled,with their nasopharyngeal swabs collected,blood samples cultured and pertussis toxin IgG detected by PCR.Results were analyzed,using SPSS 16.0 software.Results 100 out of the 148 cases were laboratorially confirmed.3,88 and 34 cases were positive,through culture,PCR or pertussis toxin IgG respectively.22 cases were both PCR and pertussis toxin IgG positive.There were significant differences between the results of IS481 PCR,days from the onset of symptoms (P＜0.01) and results of PT-IgG with the days from onset of symptoms (P＜0.01).Conclusion Since the sensitivity of culture on pertussis was low,diagnosis on the disease should be linked to the results from PCR,PT-IgG and the days from onset of symptoms.