CAJ | 학술논문

目的探讨微小核糖核酸-34a(miR-34a)在膀胱癌组织中表达的临床意义. 方法膀胱癌手术切除标本42例,按照UICC 2002 TNM临床分期系统,非肌层浸润性癌17例,肌层浸润性癌25例；按照WHO 1973分级系统,低级别18例,高级别24例.癌旁5 cm正常膀胱黏膜标本42例作为对照组.采用荧光实时定量PCR法检测组织中miR-34a的基因表达水平.应用化学方法合成成熟miR-34a瞬时转染膀胱癌T24细胞,流式细胞术检测miR-34a对T24细胞凋亡和生长增殖的影响. 结果 42例膀胱癌组织标本中miR-34a显著低表达26例(61.9％),不表达11例(26.5％),高表达5例(11.6％),且miR-34a的低表达与肿瘤大小及恶性程度相关,肿瘤越大,恶性程度越高,miR-34a表达量越低.转染miR-34a模拟物后T24细胞生长增殖明显受抑,细胞凋亡率为(9.11±0.41)％,与阴性对照组(3.05±0.29)％和空白对照组(3.73±0.55)％相比差异有统计学意义(P＜0.01). 结论低表达miR-34a可能是膀胱癌组织重要的生物学特征,并参与膀胱癌的生物学进程.
목적 탐토미소핵당핵산-34a(miR-34a)재방광암조직중표체적림상의의. 방법 방광암수술절제표본42례,안조UICC 2002 TNM림상분기계통,비기층침윤성암17례,기층침윤성암25례；안조WHO 1973분급계통,저급별18례,고급별24례.암방5 cm정상방광점막표본42례작위대조조.채용형광실시정량PCR법검측조직중miR-34a적기인표체수평.응용화학방법합성성숙miR-34a순시전염방광암T24세포,류식세포술검측miR-34a대T24세포조망화생장증식적영향. 결과 42례방광암조직표본중miR-34a현저저표체26례(61.9％),불표체11례(26.5％),고표체5례(11.6％),차miR-34a적저표체여종류대소급악성정도상관,종류월대,악성정도월고,miR-34a표체량월저.전염miR-34a모의물후T24세포생장증식명현수억,세포조망솔위(9.11±0.41)％,여음성대조조(3.05±0.29)％화공백대조조(3.73±0.55)％상비차이유통계학의의(P＜0.01). 결론 저표체miR-34a가능시방광암조직중요적생물학특정,병삼여방광암적생물학진정.
Objective To investigate the expression and biological function of microRNA-34a (miR-34a) in bladder cancer.Methods Forty-two cases of bladder cancer specimen,including 17 noninvasive carcinoma and 25 muscle invasive carcinoma classified by UICC 2002 TNM,or 18 low-grade and 24 high-grade classified by WHO 1973.Meanwhile,mucosa adjacent to the carcinoma was selected as normal control.The gene expression of miR-34a was determined using real-time quantitative polymerase chain reaction in 42 cases of bladder carcinoma samples.Mature mimics of miR-34a were chemically synthesized and transiently transfected-intoT24 bladder cancer cells.The effects of miR-34a on apoptosis and proliferation in T24 cells were evaluatedby flow cytometry and MTS respectively.Results 61.9％ of carcinoma samples showed low expression of miR-34a,which was correlated with the malignancy and tumor size of bladder carcinoma.26.5％ (11 cases) was negative and 11.6％ (5 cases) showed high expression.Furthermore,upregulation of miR-34a in T24 cells contributed to cell growth and apoptosis.The apoptosis rate of T24 cells was (9.11 ± 0.41)％,which had significant difference compared with NC mimics and blank control group respectively (P ＜ 0.01).Conclusion The relative low expression of miRNA-34a may be involved in the tumorigenesis and development of bladder carcinoma.