CAJ | 학술논문

目的研究可转运紫杉醇的多聚体纳米粒对膀胱癌的细胞毒作用. 方法应用微乳化方法制备载紫杉醇的聚乳酸-羟基乙酸共聚物(PLGA)纳米粒,以聚乙烯醇(PVA)为表面稳定剂,并以转铁蛋白(Tf)表面修饰,分析纳米粒的大小、表面电荷、载药浓度、药物释放曲线以及空白纳米粒的安全性,观察紫杉醇溶液及载紫杉醇纳米粒对膀胱癌细胞系J-82的细胞毒作用. 结果 PVA纳米粒直径约200 nm,Z电势约-24 mV,载药浓度约6.5％ (w/w),药物释放曲线为双相性.Tf表面修饰的纳米粒直径比PVA纳米粒稍大,Z电势、载药浓度、药物释放曲线与PVA纳米粒相似.两种空白纳米粒不具有细胞毒性.紫杉醇溶液在50 ng/ml时J-82细胞存活率为82.0％,100 ng/ml时为36.0％.PVA纳米粒在50 ng/ml和100 ng/ml时细胞存活率分别为40.2％和15.8％,而Tf纳米粒分别为32.9％和7.2％,两种载药纳米粒抑制J-82细胞的作用明显强于紫杉醇溶液. 结论 PLGA纳米粒是安全有效的载药工具,可以明显增强紫杉醇对膀胱癌的细胞毒作用.
목적 연구가전운자삼순적다취체납미립대방광암적세포독작용. 방법 응용미유화방법제비재자삼순적취유산-간기을산공취물(PLGA)납미립,이취을희순(PVA)위표면은정제,병이전철단백(Tf)표면수식,분석납미립적대소、표면전하、재약농도、약물석방곡선이급공백납미립적안전성,관찰자삼순용액급재자삼순납미립대방광암세포계J-82적세포독작용. 결과 PVA납미립직경약200 nm,Z전세약-24 mV,재약농도약6.5％ (w/w),약물석방곡선위쌍상성.Tf표면수식적납미립직경비PVA납미립초대,Z전세、재약농도、약물석방곡선여PVA납미립상사.량충공백납미립불구유세포독성.자삼순용액재50 ng/ml시J-82세포존활솔위82.0％,100 ng/ml시위36.0％.PVA납미립재50 ng/ml화100 ng/ml시세포존활솔분별위40.2％화15.8％,이Tf납미립분별위32.9％화7.2％,량충재약납미립억제J-82세포적작용명현강우자삼순용액. 결론 PLGA납미립시안전유효적재약공구,가이명현증강자삼순대방광암적세포독작용.
Objective To formulate paclitaxel loaded polymer nanoparticle and evaluate it's application in treatment of bladder.Methods Paclitaxel loaded Poly (lactide-co-glycolide) (PLGA) nanoparticles were formulated with microemulsion method,Polyvinyl alcohol(PVA) was used as surfactant.Transferrin (Tf) was used to modify the nanoparticles.The size,Z-potential,drug loading,drug release,cytotoxicity of bland nanoparticles and paclitaxel-loaded nanoparticles on bladder cancer cell line J-82 were measured.Results The size of nanoparticles was about 200 nm,Z-potential was-24 mV,drug loading was about 6.5％ (w/w),cumulative drug release showed two phase curve.The size of Tf modified nanoparticles was a little bigger than no modified nanoparticles.The Z-potential,drug loading,drug release was similar.Two kinds of blank nanoparticles showed no cytotoxicity on bladder cancer cell line J-82.However,both paclitaxel-loaded nanoparticles had significantly higher cytotoxicity on J-82 compared to paclitaxel solution.Conclusions PLGA nanoparticle is a promising drug delivery vehicle,which could significantly improve the anticancer effect of paclitaxel on bladder cancer.