CAJ | 학술논문

目的探讨膀胱癌组织中N-钙黏附素蛋白表达与临床病理特征及预后的关系.方法采用免疫组化方法检测2003年1月至2012年4月145例膀胱尿路上皮癌组织及25例正常膀胱组织中N-钙黏附素蛋白的表达情况.145例膀胱尿路上皮癌患者中,男104例,女41例.年龄49～83岁,中位年龄70岁,其中≤65岁57例,＞65岁88例.肿瘤大小≤3 cm 79例,＞3 cm 66例.肿瘤单发86例,多发59例.病理分级G1级44例,G2～G3级101例;分期(AJCC2002标准)Ta～T1期94例,T2～T4期51例.分析N-钙黏附素蛋白表达与膀胱癌分级、分期、数目、大小、预后及患者年龄、性别的关系. 结果 145例膀胱癌组织中N-钙黏附素蛋白表达阳性率为54.2％(76/145),正常组织中N-钙黏附素蛋白表达阳性率为12.0％(3/25),两组间差异有统计学意义(P＜0.01).G1级和G2～ G3级别膀胱癌中N-钙黏附素蛋白表达阳性率分别为38.6％ (17/44)、58.4％(59/101),差异有统计学意义(P=0.028).非肌层浸润性癌和肌层浸润性癌组织中N-钙黏附素蛋白表达阳性率分别为45.7％(43/94)和64.7％(33/51),差异有统计学意义(P=0.029).N-钙黏附素蛋白表达与患者年龄、性别、肿瘤的大小及数目无明显相关性(P＞0.05).肌层浸润性癌患者随访4～103个月,平均42.5个月,N-钙黏附素蛋白阳性表达患者总生存率24.2％ (8/33),N-钙黏附素蛋白阴性表达患者总生存率66.7％(12/18),Kaplan-Meier生存分析显示,两组间总生存率差异有统计学意义(P=0.002 2).多因素Cox回归分析显示N-钙黏附素蛋白表达是膀胱癌预后的重要因素. 结论 N-钙黏附素蛋白表达在膀胱癌组织中明显高于正常组织,N-钙黏附素蛋白的检测有助于膀胱癌的诊断及预后评估.
목적 탐토방광암조직중N-개점부소단백표체여림상병리특정급예후적관계.방법 채용면역조화방법검측2003년1월지2012년4월145례방광뇨로상피암조직급25례정상방광조직중N-개점부소단백적표체정황.145례방광뇨로상피암환자중,남104례,녀41례.년령49～83세,중위년령70세,기중≤65세57례,＞65세88례.종류대소≤3 cm 79례,＞3 cm 66례.종류단발86례,다발59례.병리분급G1급44례,G2～G3급101례;분기(AJCC2002표준)Ta～T1기94례,T2～T4기51례.분석N-개점부소단백표체여방광암분급、분기、수목、대소、예후급환자년령、성별적관계. 결과 145례방광암조직중N-개점부소단백표체양성솔위54.2％(76/145),정상조직중N-개점부소단백표체양성솔위12.0％(3/25),량조간차이유통계학의의(P＜0.01).G1급화G2～ G3급별방광암중N-개점부소단백표체양성솔분별위38.6％ (17/44)、58.4％(59/101),차이유통계학의의(P=0.028).비기층침윤성암화기층침윤성암조직중N-개점부소단백표체양성솔분별위45.7％(43/94)화64.7％(33/51),차이유통계학의의(P=0.029).N-개점부소단백표체여환자년령、성별、종류적대소급수목무명현상관성(P＞0.05).기층침윤성암환자수방4～103개월,평균42.5개월,N-개점부소단백양성표체환자총생존솔24.2％ (8/33),N-개점부소단백음성표체환자총생존솔66.7％(12/18),Kaplan-Meier생존분석현시,량조간총생존솔차이유통계학의의(P=0.002 2).다인소Cox회귀분석현시N-개점부소단백표체시방광암예후적중요인소. 결론 N-개점부소단백표체재방광암조직중명현고우정상조직,N-개점부소단백적검측유조우방광암적진단급예후평고.
Objective To explore the expression and significance of N-cadherin in urothelial bladder cancer and analyse its relation to clinicpathologic and prognosis of bladder cancer.Methods The expression of N-cadherin in 145 urothelial bladder cancer and 25 normal bladder tissues was detected by immunuhistochemisty,and correlations between N-cadherin and clinicopathologic features were analysed.Results The positive rate of N-cadherin protein was significantly higher in bladder cancer than in normal bladder tissue (P＜0.01).The positive rate of N-cadherin protein was 38.6％ in G1 bladder cancer,58.4％ in G2-G3 bladder cancer,and its difference was significant (P =0.028).The expression was significantly lower in non-muscle-invasive bladder cancer than in muscle invasive bladder cancer (45.7％ vs 64.7％,P=0.029).The muscle-invasive bladder cancer patients were followed up 4-103 months.Among those,the overall survival with positive expression of N-cadherin protein was 24.2％ (8/33),and the overall survival with negative expression of N-cadherin protein was 66.7 ％ (12/18).Kaplan-Meier analysis showed the positive expression of N-cadherin was significantly associated with overall survival of patients with muscle-invasive-bladder cancer (P=0.002 2).Multivariate Cox analysis showed that N-cadherin expression was an important prognostic factor.Conclusions The expression of N-cadherin protein was high in bladder cancer.The detection of the expression of N-cadherin protein is associated with the diagnosis and prognosis of bladder cancer.