中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2014年
5期
363-367
,共5页
李小四%叶建中%马传玲%吴庆%张亚培%凌志远%张威%曹建明%周铁丽
李小四%葉建中%馬傳玲%吳慶%張亞培%凌誌遠%張威%曹建明%週鐵麗
리소사%협건중%마전령%오경%장아배%릉지원%장위%조건명%주철려
不育症%泌尿生殖道感染%金黄色葡萄球菌%葡萄球菌染色体mec基因盒%毒力
不育癥%泌尿生殖道感染%金黃色葡萄毬菌%葡萄毬菌染色體mec基因盒%毒力
불육증%비뇨생식도감염%금황색포도구균%포도구균염색체mec기인합%독력
Infertility%Urogenital tract infection%Staphylococcus aureus%Staphylococcal Cassette Chromosome mec%Virulence
目的 探讨泌尿生殖道感染的不育症患者精液中分离出的金黄色葡萄球菌对精子活力的影响及其携带的毒力基因谱特征. 方法 收集2009年12月至2011年1月收治的不育症患者精液中分离出的金黄色葡萄球菌89株,以金黄色葡萄球菌ATCC29213为阴性对照菌,采用计算机辅助生殖分析系统Sperm Class Analyzer V.3.2.0,按照WHO(2010版)推荐的精液分析方法,观察89株金黄色葡萄球菌对体检合格的育龄男子正常精子活力的影响,进行精子活动力评级;琼脂稀释法检测喹诺酮类、磺胺类等抗菌药物对实验菌株的最低抑菌浓度(minimum inhibitory concentration,MIC);采用头孢西丁纸片扩散法和mecA基因扩增法确定其中的耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA);采用多重PCR法对MRSA菌株进行mec耐药基因盒(staphylococcal cassette chromosome mec,SCCmec)分型;采用普通PCR法检测金黄色葡萄球菌的毒力基因. 结果 本组89株金黄色葡萄球菌与ATCC29213阴性对照菌比较,22株能引起精子活力明显下降(P＜0.05);67株对精子活力的影响与ATCC29213阴性对照菌比较差异无统计学意义(P＞0.05).89株金黄色葡萄球菌对利奈唑胺、万古霉素和呋喃妥因全部敏感,对氟喹诺酮类(环丙沙星、左氧氟沙星)、红霉素、庆大霉素、四环素表现为较高的耐药性.本组89株金黄色葡萄球菌中,51株确定为MRSA,其中SCCmec Ⅰ型2株,SCCmecⅢ型12株,SCCmecⅣ型2株,SCCmecⅤ型25株,10株未能分型.PCR法检测金黄色葡萄球菌的14个毒力基因,发现22株携带hlg毒力基因、3株携带see毒力基因、1株携带sea毒力基因、1株携带seg毒力基因、1株携带sei毒力基因,1株同时携带hlg和sea基因,1株同时携带seg和sei基因. 结论 泌尿生殖道感染的不育症患者精液中分离的金黄色葡萄球菌能明显降低正常精子活力;本组金黄色葡萄球菌的主要毒力基因是γ-溶血素编码基因hlg,还有散在分布的肠毒素编码基因sea、see、seg和sei.
目的 探討泌尿生殖道感染的不育癥患者精液中分離齣的金黃色葡萄毬菌對精子活力的影響及其攜帶的毒力基因譜特徵. 方法 收集2009年12月至2011年1月收治的不育癥患者精液中分離齣的金黃色葡萄毬菌89株,以金黃色葡萄毬菌ATCC29213為陰性對照菌,採用計算機輔助生殖分析繫統Sperm Class Analyzer V.3.2.0,按照WHO(2010版)推薦的精液分析方法,觀察89株金黃色葡萄毬菌對體檢閤格的育齡男子正常精子活力的影響,進行精子活動力評級;瓊脂稀釋法檢測喹諾酮類、磺胺類等抗菌藥物對實驗菌株的最低抑菌濃度(minimum inhibitory concentration,MIC);採用頭孢西丁紙片擴散法和mecA基因擴增法確定其中的耐甲氧西林金黃色葡萄毬菌(methicillin-resistant Staphylococcus aureus,MRSA);採用多重PCR法對MRSA菌株進行mec耐藥基因盒(staphylococcal cassette chromosome mec,SCCmec)分型;採用普通PCR法檢測金黃色葡萄毬菌的毒力基因. 結果 本組89株金黃色葡萄毬菌與ATCC29213陰性對照菌比較,22株能引起精子活力明顯下降(P＜0.05);67株對精子活力的影響與ATCC29213陰性對照菌比較差異無統計學意義(P＞0.05).89株金黃色葡萄毬菌對利奈唑胺、萬古黴素和呋喃妥因全部敏感,對氟喹諾酮類(環丙沙星、左氧氟沙星)、紅黴素、慶大黴素、四環素錶現為較高的耐藥性.本組89株金黃色葡萄毬菌中,51株確定為MRSA,其中SCCmec Ⅰ型2株,SCCmecⅢ型12株,SCCmecⅣ型2株,SCCmecⅤ型25株,10株未能分型.PCR法檢測金黃色葡萄毬菌的14箇毒力基因,髮現22株攜帶hlg毒力基因、3株攜帶see毒力基因、1株攜帶sea毒力基因、1株攜帶seg毒力基因、1株攜帶sei毒力基因,1株同時攜帶hlg和sea基因,1株同時攜帶seg和sei基因. 結論 泌尿生殖道感染的不育癥患者精液中分離的金黃色葡萄毬菌能明顯降低正常精子活力;本組金黃色葡萄毬菌的主要毒力基因是γ-溶血素編碼基因hlg,還有散在分佈的腸毒素編碼基因sea、see、seg和sei.
목적 탐토비뇨생식도감염적불육증환자정액중분리출적금황색포도구균대정자활력적영향급기휴대적독력기인보특정. 방법 수집2009년12월지2011년1월수치적불육증환자정액중분리출적금황색포도구균89주,이금황색포도구균ATCC29213위음성대조균,채용계산궤보조생식분석계통Sperm Class Analyzer V.3.2.0,안조WHO(2010판)추천적정액분석방법,관찰89주금황색포도구균대체검합격적육령남자정상정자활력적영향,진행정자활동력평급;경지희석법검측규낙동류、광알류등항균약물대실험균주적최저억균농도(minimum inhibitory concentration,MIC);채용두포서정지편확산법화mecA기인확증법학정기중적내갑양서림금황색포도구균(methicillin-resistant Staphylococcus aureus,MRSA);채용다중PCR법대MRSA균주진행mec내약기인합(staphylococcal cassette chromosome mec,SCCmec)분형;채용보통PCR법검측금황색포도구균적독력기인. 결과 본조89주금황색포도구균여ATCC29213음성대조균비교,22주능인기정자활력명현하강(P＜0.05);67주대정자활력적영향여ATCC29213음성대조균비교차이무통계학의의(P＞0.05).89주금황색포도구균대리내서알、만고매소화부남타인전부민감,대불규낙동류(배병사성、좌양불사성)、홍매소、경대매소、사배소표현위교고적내약성.본조89주금황색포도구균중,51주학정위MRSA,기중SCCmec Ⅰ형2주,SCCmecⅢ형12주,SCCmecⅣ형2주,SCCmecⅤ형25주,10주미능분형.PCR법검측금황색포도구균적14개독력기인,발현22주휴대hlg독력기인、3주휴대see독력기인、1주휴대sea독력기인、1주휴대seg독력기인、1주휴대sei독력기인,1주동시휴대hlg화sea기인,1주동시휴대seg화sei기인. 결론 비뇨생식도감염적불육증환자정액중분리적금황색포도구균능명현강저정상정자활력;본조금황색포도구균적주요독력기인시γ-용혈소편마기인hlg,환유산재분포적장독소편마기인sea、see、seg화sei.
Objective To study the effect of Staphylococcus aureus from sperm of infertile male patients with genital urinary infection on spermatozoa,as well as the virulence genes spectrum of these Staphylococcus aureus.Methods A total of 89 strains of Staphylococcus aureus were isolated from infertility males'sperm between December 2009 and January 2011.The ATCC29213 strain of Staphylococcus aureus were considered as negative control.Computer assisted system:Sperm Class Analyzer V.3.2.0 was used to screen the influence of all strain on the robust semen according to the semen analysis method in the WHO (2010) guideline.The antimicrobial susceptibility test of all isolated strains was performed by agar dilution method including the common antibiotics,quinolones,sulfonamides and so on.The methicillin-resistant Staphylococcus aureus (MRSA) were determined by Cefoxitin disk diffusion and Polymerase Chain Reaction (PCR) for mecA gene.SCCmec genotypes were conformed via Multiplex-PCR and DNA sequencing,the virulence genes were also detected by PCR method.Results Twenty-two strains of Staphylococcus aureus could significiantly weaken the mobility of spermatozoahe than the ability in the negative control Staphylococcus aureus ATCC29213 (P＜0.05).The remainning 67 isolates could not inhibit the activity of sperm (P＞ 0.05).All the isolates were sensitive to the vancomycin,linezolid and furadantin.Instead,they displayed high-level antibiotics resistance to fluoroquinolones (Ciprofloxacin,levofloxacin),erythromycin,gentamicin and tetracycline.Among the 51 MRSA isolates,the SCCmec genotypes were shown as follows:SCCmec Ⅰ was indentified in 2 isolates,SCCmec Ⅲ in 12 isolates,SCCmec Ⅳ in 2 isolates,SCCmec Ⅴ in 25 isolates,the remaining ten were undefined genotyping.The putative 14 virulence determinants in the 89 isolates were detected by PCR,results were shown as follows:22 isolates of S.aureus had gene for hlg,3 isolates for see,1 isolate for sea,1 isolate for seg,1 isolate for sei,only 1 isolate had genes for hlg and sea simultaneously and 1 for seg and sei simultaneously.Conclusions The Staphylococcus aureus isolated from urogenital tract of infertile male patients with genitourinary infection could inhibit significantly the mobility of spermatozoa; In our research,the primary virulence determinate was hlg encoding γ-hemolysin,and the gene sea,see,seg and sei,which encoded enterotoxins were also scatteredly distributed in the Staphylococcus aureus.
