中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2014年
5期
368-372
,共5页
氯胺酮%大鼠%泌尿系损害%组胺受体H1
氯胺酮%大鼠%泌尿繫損害%組胺受體H1
록알동%대서%비뇨계손해%조알수체H1
Ketamine%Rats%Urinary system dysfunction%Histamine receptor H1
目的 探讨长期腹腔注射氯胺酮后大鼠肾脏和膀胱组织中组胺受体H1的表达及其临床意义. 方法 2012年5-12月选取SPF级雄性SD大鼠60只,2月龄,体质量(200± 10)g.随机数字表法分为A、B两组,每组30只.A组以氯胺酮100 mg/kg腹腔注射,隔日1次;B组以相应量生理盐水腹腔注射,隔日1次.每周称体质量1次,校正用药量.分别于用药2、4、6个月后,两组各随机抽取10只,记录2h排尿次数,然后放入代谢笼24h,收集尿液,检测尿液中钠、钾含量.心尖采血,检测血肌酐水平,然后处死.切取大鼠肾脏、膀胱,应用免疫组化S-P法,标记大鼠肾脏及膀胱组织中组胺受体H1,用Imagepro-plus6.0图像分析软件计算各组中H1受体的平均吸光度A值,作为H1受体的表达水平.比较两组大鼠的尿量,2h排尿次数,24h钠、钾排泄量,血肌酐浓度及H1受体平均吸光度A值的差异. 结果 用药6个月后,观察大鼠膀胱组织病理变化证实建模成功率为90％(9/10).A、B组24h平均尿量分别为(15.9± 1.3)、(10.1±0.8) ml;2h平均排尿次数分别为(6.9±1.4)、(3.0±0.5)次,差异均有统计学意义(P＜0.05).A、B组24 h尿钠含量分别为(1.7±0.1)、(1.0±0.1)mmol,尿钾含量分别为(1.1±0.1)、(2.6±0.1)mmol,组间比较差异均有统计学意义(P＜0.05).A、B组血肌酐分别为(60.5±6.8)、(58.1 ±3.9) μmol/L,差异无统计学意义(P＞0.05).A组用药2、4、6个月后,肾脏H1受体的表达水平分别为0.008±0.001、0.016±0.001、0.023±0.004;B组分别为0.003±0.001、0.004±0.002、0.003±0.001,两组比较差异均有统计学意义(P＜0.05).A组用药2、4、6个月后,膀胱组织H1的表达水平分别为0.017±0.006、0.031±0.012、0.036±0.007;B组分别为0.015±0.007、0.016±0.005、0.016±0.004,两组比较差异均有统计学意义(P＜0.05). 结论 长期腹腔注射氯胺酮可引起大鼠的膀胱和肾脏损害,其机制与H1受体过表达有关.大鼠血肌酐尚未出现异常时,每日尿钠、钾含量可能成为早期衡量氯胺酮损害肾脏的敏感指标.
目的 探討長期腹腔註射氯胺酮後大鼠腎髒和膀胱組織中組胺受體H1的錶達及其臨床意義. 方法 2012年5-12月選取SPF級雄性SD大鼠60隻,2月齡,體質量(200± 10)g.隨機數字錶法分為A、B兩組,每組30隻.A組以氯胺酮100 mg/kg腹腔註射,隔日1次;B組以相應量生理鹽水腹腔註射,隔日1次.每週稱體質量1次,校正用藥量.分彆于用藥2、4、6箇月後,兩組各隨機抽取10隻,記錄2h排尿次數,然後放入代謝籠24h,收集尿液,檢測尿液中鈉、鉀含量.心尖採血,檢測血肌酐水平,然後處死.切取大鼠腎髒、膀胱,應用免疫組化S-P法,標記大鼠腎髒及膀胱組織中組胺受體H1,用Imagepro-plus6.0圖像分析軟件計算各組中H1受體的平均吸光度A值,作為H1受體的錶達水平.比較兩組大鼠的尿量,2h排尿次數,24h鈉、鉀排洩量,血肌酐濃度及H1受體平均吸光度A值的差異. 結果 用藥6箇月後,觀察大鼠膀胱組織病理變化證實建模成功率為90％(9/10).A、B組24h平均尿量分彆為(15.9± 1.3)、(10.1±0.8) ml;2h平均排尿次數分彆為(6.9±1.4)、(3.0±0.5)次,差異均有統計學意義(P＜0.05).A、B組24 h尿鈉含量分彆為(1.7±0.1)、(1.0±0.1)mmol,尿鉀含量分彆為(1.1±0.1)、(2.6±0.1)mmol,組間比較差異均有統計學意義(P＜0.05).A、B組血肌酐分彆為(60.5±6.8)、(58.1 ±3.9) μmol/L,差異無統計學意義(P＞0.05).A組用藥2、4、6箇月後,腎髒H1受體的錶達水平分彆為0.008±0.001、0.016±0.001、0.023±0.004;B組分彆為0.003±0.001、0.004±0.002、0.003±0.001,兩組比較差異均有統計學意義(P＜0.05).A組用藥2、4、6箇月後,膀胱組織H1的錶達水平分彆為0.017±0.006、0.031±0.012、0.036±0.007;B組分彆為0.015±0.007、0.016±0.005、0.016±0.004,兩組比較差異均有統計學意義(P＜0.05). 結論 長期腹腔註射氯胺酮可引起大鼠的膀胱和腎髒損害,其機製與H1受體過錶達有關.大鼠血肌酐尚未齣現異常時,每日尿鈉、鉀含量可能成為早期衡量氯胺酮損害腎髒的敏感指標.
목적 탐토장기복강주사록알동후대서신장화방광조직중조알수체H1적표체급기림상의의. 방법 2012년5-12월선취SPF급웅성SD대서60지,2월령,체질량(200± 10)g.수궤수자표법분위A、B량조,매조30지.A조이록알동100 mg/kg복강주사,격일1차;B조이상응량생리염수복강주사,격일1차.매주칭체질량1차,교정용약량.분별우용약2、4、6개월후,량조각수궤추취10지,기록2h배뇨차수,연후방입대사롱24h,수집뇨액,검측뇨액중납、갑함량.심첨채혈,검측혈기항수평,연후처사.절취대서신장、방광,응용면역조화S-P법,표기대서신장급방광조직중조알수체H1,용Imagepro-plus6.0도상분석연건계산각조중H1수체적평균흡광도A치,작위H1수체적표체수평.비교량조대서적뇨량,2h배뇨차수,24h납、갑배설량,혈기항농도급H1수체평균흡광도A치적차이. 결과 용약6개월후,관찰대서방광조직병리변화증실건모성공솔위90％(9/10).A、B조24h평균뇨량분별위(15.9± 1.3)、(10.1±0.8) ml;2h평균배뇨차수분별위(6.9±1.4)、(3.0±0.5)차,차이균유통계학의의(P＜0.05).A、B조24 h뇨납함량분별위(1.7±0.1)、(1.0±0.1)mmol,뇨갑함량분별위(1.1±0.1)、(2.6±0.1)mmol,조간비교차이균유통계학의의(P＜0.05).A、B조혈기항분별위(60.5±6.8)、(58.1 ±3.9) μmol/L,차이무통계학의의(P＞0.05).A조용약2、4、6개월후,신장H1수체적표체수평분별위0.008±0.001、0.016±0.001、0.023±0.004;B조분별위0.003±0.001、0.004±0.002、0.003±0.001,량조비교차이균유통계학의의(P＜0.05).A조용약2、4、6개월후,방광조직H1적표체수평분별위0.017±0.006、0.031±0.012、0.036±0.007;B조분별위0.015±0.007、0.016±0.005、0.016±0.004,량조비교차이균유통계학의의(P＜0.05). 결론 장기복강주사록알동가인기대서적방광화신장손해,기궤제여H1수체과표체유관.대서혈기항상미출현이상시,매일뇨납、갑함량가능성위조기형량록알동손해신장적민감지표.
Objective To investigate the expression and clinical significance of H1 receptor in kidney and bladder tissue of rats after long term ketamine intraperitoneal injection.Methods This study was conducted from May 2012 to December 2012.Sixty male 2-month-old SD rats,weighted (200±10) g,were randomly divided into Group A and Group B.Each group concluded 30 rats.In the Group A,Ketamine (100 mg/kg) was given as intraperitoneal injection every other day,while normal saline (100 mg/kg) was given in Group B.The dosage was adjusted every week according to the weight of rats.After 2,4 and 6 months,10 rats from each group were randomly chosen.First,the micturition number during 2 h was recorded.Then,urine samples over a 24 h period were collected and the content of Na+ and K+ were determined.Finally,the blood samples were obtained from the apex of heart for the creatinine determination.The kidneys and bladders were harvested after the rats were sacrificed.HE staining was conducted on all the tissues.Immunohistochemical S-P method was used to detect the expression of H1 receptor in the bladder and kidney tissues from Group A and Group B.The average optical density (A Value) in each group was separately calculated by Imagepro-plus 6.0 software.All the parameters,mentioned above,were carefully compared.Results The successive rate of establishing rats model was 90％ (9/10),according to the pathological result after 6 months injection.The urine volume of 24 h in group A and B were (15.9±1.3) and (10.1±0.8) ml,respectively.Micturition frequency during 2 hours in group A and B were (6.9±1.4) and (3.0±0.5) times.The urine volume of 24 h and micturition frequency during 2 hours were significantly increased in group A (P＜ 0.05).The urine sodium within 24 h in group A was (1.7±0.1) mmol,which is increased significantly than that in group B (1.0±0.1 mmol).While the urine potassium was less in group A (1.1±0.1 mmol/d) than in group B (2.6±0.1 mmol/d) (P＜0.05).But the serum creatinine level were (60.5±6.8) and (58.1± 3.9) μmol/L in group A and B,which had no difference between the two groups (P＜0.05).The expression of H1 receptor in kidneys and bladder in group A was significantly raised compared with group B (P＜0.05).In the group A,the expression of H1 receptor level in kidney was 0.008±0.001,0.016±0.001,0.023±0.004 after 2,4 and 6 months drug used.The expression level in group A were significantly difference than that in group B (0.003±0.001,0.004±0.002,0.003±0.001) (P＜0.05) and goes up with prolonging the drug using.While in the bladder tissue,the level of H1 receptor expression was 0.017±0.006,0.031±0.012,0.036±0.007 in group A and 0.015±0.007,0.016±0.005,0.016±0.004 in group B,which could be noticed a significantly increasing in group A (P＜0.05).In 4 and 6 months,the H1 receptor expression level significantly raised than that in 2 months (P＜0.05).Conclusions Long term ketamine addiction exerts toxicity not only on the bladder but also on the kidney.The increased expression of H1 receptor in rats' kidney and bladder tissues of group A indicates that H1 receptor may be related to the ketamine-associated urinary system dysfunction.The urine sodium and potassium within 24 h may be a sensitive index for the assessment of degree of kidney damage in the early stage of ketamine-induced dysfunction than serum creatinine.
